Abstract Bone cells namely osteoblasts and osteocytes are assumed responsible for loading-induced osteogenesis. Osteocytes lie within the bone and connect to each other via cell process passing through canalicular spaces which forms a canalicular network. Fluid motion across this network acts as a medium of communication with neighbouring cells. Mechanical loading-induced pressure gradients in lacunar canalicular space (LCS) causes canalicular fluid to flow. However, it remains unclear how canalicular fluid motion…