Evaluation of loop-mediated isothermal amplification method for efficient detection of the periodontopathic bacteria Porphyromonas gingivalis Article Swipe
Meenaz N. Sangolli
,
Manohar S. Kugaji
,
Suman Kumar Ray
,
Kishore Bhat
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.4103/jisp.jisp_260_23
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.4103/jisp.jisp_260_23
Background: Periodontitis is a multifactorial, polymicrobial oral inflammatory illness brought on by oral pathogens. Porphyromonas gingivalis is a Gram-negative, obligatory anaerobic, black-pigmented coccobacillus and is regarded as a primary etiological factor in the progression of periodontitis. Rapid, highly senstitive and specific detection methods are emerging. The present study aimed to evaluate the loop-mediated isothermal amplification (LAMP) technique for efficiently detecting P . gingivalis from subgingival plaque samples of chronic periodontitis patients. Materials and Methods: This study included 50 subgingival plaque samples from patients suffering from chronic periodontitis. The DNA (Deoxyribonucleic acid) was extracted by the “modified proteinase K” method. A set of six primers, targeting the pepO gene of P . gingivalis , was used for conducting LAMP. The amplification was visualized by naked-eye detection and agarose electrophoresis. Conventional polymerase chain reaction (PCR) and real-time qantitative PCR (qPCR) were carried out by targeting the 16SrRNA (16S ribosomal ribonucleic acid) gene of P . gingivalis . Results: The results showed that LAMP detected P . gingivalis in 40 out of 50 samples (80%). Whereas, qPCR and conventional PCR technique detected P. gingivalis in 38 (76%) and 33 (66%) samples respectively. The sensitivity and specificity of the LAMP method were 94.87% and 90.90%, respectively. With qPCR, the sensitivity and specificity were found to be 92.30% and 81.81%, respectively, whereas, with conventional PCR, it was found to be 76.92% and 72.72%, respectively. Conclusion: LAMP is an efficient technique for quick, accurate, and reliable identification of P . gingivalis from subgingival plaque samples. The technique needs to be validated analytically, and further studies can be conducted by taking saliva and/or gingival crevicular fluid samples from periodontitis patients.
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- Type
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- Language
- en
- Landing Page
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- OA Status
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- References
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- OpenAlex ID
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Raw OpenAlex JSON
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https://openalex.org/W4399341540Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.4103/jisp.jisp_260_23Digital Object Identifier
- Title
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Evaluation of loop-mediated isothermal amplification method for efficient detection of the periodontopathic bacteria Porphyromonas gingivalisWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2024Year of publication
- Publication date
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2024-01-01Full publication date if available
- Authors
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Meenaz N. Sangolli, Manohar S. Kugaji, Suman Kumar Ray, Kishore BhatList of authors in order
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https://doi.org/10.4103/jisp.jisp_260_23Publisher landing page
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YesWhether a free full text is available
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diamondOpen access status per OpenAlex
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https://doi.org/10.4103/jisp.jisp_260_23Direct OA link when available
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Porphyromonas gingivalis, Loop-mediated isothermal amplification, Bacteria, Microbiology, Loop (graph theory), Chemistry, Biology, Mathematics, Genetics, Combinatorics, Biochemistry, DNATop concepts (fields/topics) attached by OpenAlex
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0Total citation count in OpenAlex
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10Other works algorithmically related by OpenAlex
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| referenced_works | https://openalex.org/W2000749448, https://openalex.org/W2279029296, https://openalex.org/W2550762460, https://openalex.org/W4200387359, https://openalex.org/W2801235907, https://openalex.org/W1783598356, https://openalex.org/W1969752885, https://openalex.org/W2953728550, https://openalex.org/W91057871, https://openalex.org/W3126277197, https://openalex.org/W2026302152, https://openalex.org/W2160057022, https://openalex.org/W1997824772, https://openalex.org/W2095691138, https://openalex.org/W2009050027, https://openalex.org/W2163702408, https://openalex.org/W2069994724, https://openalex.org/W2903347362, https://openalex.org/W2889819256, https://openalex.org/W1976329656, https://openalex.org/W2142585314, https://openalex.org/W2103456695, https://openalex.org/W2141633648, https://openalex.org/W2105275554, https://openalex.org/W3184781691, https://openalex.org/W3000172394, https://openalex.org/W2770752141, https://openalex.org/W4384037730, https://openalex.org/W3135168675, https://openalex.org/W6850902116, https://openalex.org/W4205270223, https://openalex.org/W2174784305, https://openalex.org/W2759773098, https://openalex.org/W2055854276, https://openalex.org/W2108060898, https://openalex.org/W1895298047, https://openalex.org/W1997925861, https://openalex.org/W2973051408, https://openalex.org/W2169732730, https://openalex.org/W1967702035, https://openalex.org/W2051904811, https://openalex.org/W6784448538, https://openalex.org/W2884219040, https://openalex.org/W4321097807, https://openalex.org/W1984966664, https://openalex.org/W1975801865, https://openalex.org/W2066385972, https://openalex.org/W1690462967, https://openalex.org/W2066563672, https://openalex.org/W4384202127, https://openalex.org/W4387616642, https://openalex.org/W3173358714, https://openalex.org/W4303628118, https://openalex.org/W2105472444, https://openalex.org/W2044545884, https://openalex.org/W1969369067, https://openalex.org/W2137591193, https://openalex.org/W2129109142, https://openalex.org/W6775622204, https://openalex.org/W3014867362, https://openalex.org/W4361296751, https://openalex.org/W3093886123 |
| referenced_works_count | 62 |
| abstract_inverted_index., | 113 |
| abstract_inverted_index.. | 62, 111, 153, 155, 164, 244 |
| abstract_inverted_index.A | 100 |
| abstract_inverted_index.P | 61, 110, 152, 163, 243 |
| abstract_inverted_index.a | 4, 18, 28 |
| abstract_inverted_index.33 | 186 |
| abstract_inverted_index.38 | 183 |
| abstract_inverted_index.40 | 167 |
| abstract_inverted_index.50 | 78, 170 |
| abstract_inverted_index.P. | 180 |
| abstract_inverted_index.an | 233 |
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| abstract_inverted_index.be | 212, 225, 254, 261 |
| abstract_inverted_index.by | 12, 94, 123, 142, 263 |
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| abstract_inverted_index.it | 221 |
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| abstract_inverted_index.PCR | 137, 177 |
| abstract_inverted_index.The | 46, 88, 119, 157, 190, 250 |
| abstract_inverted_index.and | 24, 40, 73, 126, 134, 175, 185, 192, 200, 207, 214, 227, 239, 257 |
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| abstract_inverted_index.K” | 98 |
| abstract_inverted_index.LAMP | 161, 196, 231 |
| abstract_inverted_index.PCR, | 220 |
| abstract_inverted_index.This | 75 |
| abstract_inverted_index.With | 203 |
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| abstract_inverted_index.pepO | 107 |
| abstract_inverted_index.qPCR | 174 |
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| abstract_inverted_index.were | 139, 198, 209 |
| abstract_inverted_index.with | 218 |
| abstract_inverted_index.(66%) | 187 |
| abstract_inverted_index.(76%) | 184 |
| abstract_inverted_index.(PCR) | 133 |
| abstract_inverted_index.LAMP. | 118 |
| abstract_inverted_index.acid) | 91, 149 |
| abstract_inverted_index.aimed | 49 |
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| abstract_inverted_index.qPCR, | 204 |
| abstract_inverted_index.study | 48, 76 |
| abstract_inverted_index.(80%). | 172 |
| abstract_inverted_index.(LAMP) | 56 |
| abstract_inverted_index.(qPCR) | 138 |
| abstract_inverted_index.76.92% | 226 |
| abstract_inverted_index.92.30% | 213 |
| abstract_inverted_index.94.87% | 199 |
| abstract_inverted_index.Rapid, | 37 |
| abstract_inverted_index.and/or | 266 |
| abstract_inverted_index.factor | 31 |
| abstract_inverted_index.highly | 38 |
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| abstract_inverted_index.saliva | 265 |
| abstract_inverted_index.showed | 159 |
| abstract_inverted_index.taking | 264 |
| abstract_inverted_index.16SrRNA | 145 |
| abstract_inverted_index.72.72%, | 228 |
| abstract_inverted_index.81.81%, | 215 |
| abstract_inverted_index.90.90%, | 201 |
| abstract_inverted_index.agarose | 127 |
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| abstract_inverted_index.chronic | 69, 86 |
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| abstract_inverted_index.studies | 259 |
| abstract_inverted_index.Methods: | 74 |
| abstract_inverted_index.Results: | 156 |
| abstract_inverted_index.Whereas, | 173 |
| abstract_inverted_index.detected | 162, 179 |
| abstract_inverted_index.evaluate | 51 |
| abstract_inverted_index.gingival | 267 |
| abstract_inverted_index.included | 77 |
| abstract_inverted_index.patients | 83 |
| abstract_inverted_index.primers, | 104 |
| abstract_inverted_index.reaction | 132 |
| abstract_inverted_index.regarded | 26 |
| abstract_inverted_index.reliable | 240 |
| abstract_inverted_index.samples. | 249 |
| abstract_inverted_index.specific | 41 |
| abstract_inverted_index.whereas, | 217 |
| abstract_inverted_index.Abstract: | 0 |
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| abstract_inverted_index.accurate, | 238 |
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| abstract_inverted_index.detecting | 60 |
| abstract_inverted_index.detection | 42, 125 |
| abstract_inverted_index.efficient | 234 |
| abstract_inverted_index.emerging. | 45 |
| abstract_inverted_index.extracted | 93 |
| abstract_inverted_index.naked-eye | 124 |
| abstract_inverted_index.patients. | 71, 273 |
| abstract_inverted_index.real-time | 135 |
| abstract_inverted_index.ribosomal | 147 |
| abstract_inverted_index.suffering | 84 |
| abstract_inverted_index.targeting | 105, 143 |
| abstract_inverted_index.technique | 57, 178, 235, 251 |
| abstract_inverted_index.validated | 255 |
| abstract_inverted_index.anaerobic, | 21 |
| abstract_inverted_index.conducting | 117 |
| abstract_inverted_index.crevicular | 268 |
| abstract_inverted_index.gingivalis | 16, 63, 112, 154, 165, 181, 245 |
| abstract_inverted_index.isothermal | 54 |
| abstract_inverted_index.obligatory | 20 |
| abstract_inverted_index.pathogens. | 14 |
| abstract_inverted_index.polymerase | 130 |
| abstract_inverted_index.proteinase | 97 |
| abstract_inverted_index.senstitive | 39 |
| abstract_inverted_index.visualized | 122 |
| abstract_inverted_index.Background: | 1 |
| abstract_inverted_index.Conclusion: | 230 |
| abstract_inverted_index.efficiently | 59 |
| abstract_inverted_index.etiological | 30 |
| abstract_inverted_index.progression | 34 |
| abstract_inverted_index.qantitative | 136 |
| abstract_inverted_index.ribonucleic | 148 |
| abstract_inverted_index.sensitivity | 191, 206 |
| abstract_inverted_index.specificity | 193, 208 |
| abstract_inverted_index.subgingival | 65, 79, 247 |
| abstract_inverted_index.“modified | 96 |
| abstract_inverted_index.Conventional | 129 |
| abstract_inverted_index.conventional | 176, 219 |
| abstract_inverted_index.inflammatory | 8 |
| abstract_inverted_index.Periodontitis | 2 |
| abstract_inverted_index.Porphyromonas | 15 |
| abstract_inverted_index.amplification | 55, 120 |
| abstract_inverted_index.analytically, | 256 |
| abstract_inverted_index.coccobacillus | 23 |
| abstract_inverted_index.loop-mediated | 53 |
| abstract_inverted_index.periodontitis | 70, 272 |
| abstract_inverted_index.polymicrobial | 6 |
| abstract_inverted_index.respectively, | 216 |
| abstract_inverted_index.respectively. | 189, 202, 229 |
| abstract_inverted_index.Gram-negative, | 19 |
| abstract_inverted_index.identification | 241 |
| abstract_inverted_index.periodontitis. | 36, 87 |
| abstract_inverted_index.black-pigmented | 22 |
| abstract_inverted_index.multifactorial, | 5 |
| abstract_inverted_index.electrophoresis. | 128 |
| abstract_inverted_index.(Deoxyribonucleic | 90 |
| cited_by_percentile_year | |
| countries_distinct_count | 1 |
| institutions_distinct_count | 4 |
| sustainable_development_goals[0].id | https://metadata.un.org/sdg/3 |
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| citation_normalized_percentile.is_in_top_10_percent | False |