Evaluation of recombinant monoclonal antibody SVmab1 binding to NaV1.7 target sequences and block of human NaV1.7 currents Article Swipe
Dong Liu
,
Mandy Tseng
,
Linda F. Epstein
,
Lydia Green
,
Brian Chan
,
Brian D. Soriano
,
Désirée Lim
,
Oscar Pan
,
Christopher M. Murawsky
,
Chadwick King
,
Bryan D. Moyer
·
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.12688/f1000research.9918.1
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.12688/f1000research.9918.1
Identification of small and large molecule pain therapeutics that target the genetically validated voltage-gated sodium channel NaV1.7 is a challenging endeavor under vigorous pursuit. The monoclonal antibody SVmab1 was recently published to bind the NaV1.7 DII voltage sensor domain and block human NaV1.7 sodium currents in heterologous cells. We produced purified SVmab1 protein based on publically available sequence information, and evaluated its activity in a battery of binding and functional assays. Herein, we report that our recombinant SVmAb1 does not bind peptide immunogen or purified NaV1.7 DII voltage sensor domain via ELISA, and does not bind NaV1.7 in live HEK293, U-2 OS, and CHO-K1 cells via FACS. Whole cell manual patch clamp electrophysiology protocols interrogating diverse NaV1.7 gating states in HEK293 cells, revealed that recombinant SVmab1 does not block NaV1.7 currents to an extent greater than observed with an isotype matched control antibody. Collectively, our results show that recombinant SVmab1 monoclonal antibody does not bind NaV1.7 target sequences or specifically inhibit NaV1.7 current.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.12688/f1000research.9918.1
- https://f1000research.com/articles/5-2764/v1/pdf
- OA Status
- gold
- Cited By
- 32
- References
- 17
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2549842750
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W2549842750Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.12688/f1000research.9918.1Digital Object Identifier
- Title
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Evaluation of recombinant monoclonal antibody SVmab1 binding to NaV1.7 target sequences and block of human NaV1.7 currentsWork title
- Type
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preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
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2016Year of publication
- Publication date
-
2016-11-25Full publication date if available
- Authors
-
Dong Liu, Mandy Tseng, Linda F. Epstein, Lydia Green, Brian Chan, Brian D. Soriano, Désirée Lim, Oscar Pan, Christopher M. Murawsky, Chadwick King, Bryan D. MoyerList of authors in order
- Landing page
-
https://doi.org/10.12688/f1000research.9918.1Publisher landing page
- PDF URL
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https://f1000research.com/articles/5-2764/v1/pdfDirect link to full text PDF
- Open access
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YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://f1000research.com/articles/5-2764/v1/pdfDirect OA link when available
- Concepts
-
Biology, Recombinant DNA, Monoclonal antibody, Antibody, Biochemistry, Genetics, GeneTop concepts (fields/topics) attached by OpenAlex
- Cited by
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32Total citation count in OpenAlex
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2023: 2, 2021: 4, 2020: 5, 2019: 5, 2018: 9Per-year citation counts (last 5 years)
- References (count)
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17Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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