Arteriosclerosis Thrombosis and Vascular Biology • Vol 43 • No 1
Ex Vivo Modeling of the PC (Protein C) Pathway Using Endothelial Cells and Plasma: A Personalized Approach
November 2022 • Nadine Schwarz, Jens Müller, Hamideh Yadegari, Hannah L. McRae, Sara Reda, Nasim Shahidi Hamedani, Johannes Oldenburg, Bernd Pötzsch, Heiko Rühl
Background: The endothelial cell–dependent PC (protein C) pathway is critically involved in the regulation of coagulation, anti-inflammatory, and cytoprotective signaling. Its reactivity shows high interindividual variability, and it contributes to prothrombotic disorders, such as the FVL (factor V Leiden) mutation. Methods: Endothelial colony–forming cells (ECFCs) were isolated from heparinized peripheral blood from healthy individuals and FVL carriers. Confluent monolayers of ECFCs were overlaid with plasma, and…