Expanding the DNA editing toolbox: Novel lambda integrase variants targeting microalgal and human genome sequences Article Swipe
Jia Wei Siau
,
Asim Azhar Siddiqui
,
Sze Yi Lau
,
Srinivasaraghavan Kannan
,
Sabrina Peter
,
Yingying Zeng
,
Chandra Verma
,
Peter Dröge
,
Farid J. Ghadessy
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1371/journal.pone.0292479
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1371/journal.pone.0292479
Recombinase enzymes are extremely efficient at integrating very large DNA fragments into target genomes. However, intrinsic sequence specificities curtail their use to DNA sequences with sufficient homology to endogenous target motifs. Extensive engineering is therefore required to broaden applicability and robustness. Here, we describe the directed evolution of novel lambda integrase variants capable of editing exogenous target sequences identified in the diatom Phaeodactylum tricornutum and the algae Nannochloropsis oceanica . These microorganisms hold great promise as conduits for green biomanufacturing and carbon sequestration. The evolved enzyme variants show >1000-fold switch in specificity towards the non-natural target sites when assayed in vitro . A single-copy target motif in the human genome with homology to the Nannochloropsis oceanica site can also be efficiently targeted using an engineered integrase, both in vitro and in human cells. The developed integrase variants represent useful additions to the DNA editing toolbox, with particular application for targeted genomic insertion of large DNA cargos.
Related Topics
Concepts
Integrase
Biology
Phaeodactylum tricornutum
Genome
Computational biology
DNA
Recombinase
Genome engineering
Synthetic biology
Genetics
Genome editing
Gene
Diatom
Botany
Recombination
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1371/journal.pone.0292479
- https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0292479&type=printable
- OA Status
- gold
- Cited By
- 1
- References
- 40
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4391773730
All OpenAlex metadata
Raw OpenAlex JSON
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https://openalex.org/W4391773730Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1371/journal.pone.0292479Digital Object Identifier
- Title
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Expanding the DNA editing toolbox: Novel lambda integrase variants targeting microalgal and human genome sequencesWork title
- Type
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articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
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2024Year of publication
- Publication date
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2024-02-13Full publication date if available
- Authors
-
Jia Wei Siau, Asim Azhar Siddiqui, Sze Yi Lau, Srinivasaraghavan Kannan, Sabrina Peter, Yingying Zeng, Chandra Verma, Peter Dröge, Farid J. GhadessyList of authors in order
- Landing page
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https://doi.org/10.1371/journal.pone.0292479Publisher landing page
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https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0292479&type=printableDirect link to full text PDF
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YesWhether a free full text is available
- OA status
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goldOpen access status per OpenAlex
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https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0292479&type=printableDirect OA link when available
- Concepts
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Integrase, Biology, Phaeodactylum tricornutum, Genome, Computational biology, DNA, Recombinase, Genome engineering, Synthetic biology, Genetics, Genome editing, Gene, Diatom, Botany, RecombinationTop concepts (fields/topics) attached by OpenAlex
- Cited by
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1Total citation count in OpenAlex
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2024: 1Per-year citation counts (last 5 years)
- References (count)
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40Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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