Exploration of microbiome diversity of stacked fermented grains by flow cytometry and cell sorting Article Swipe
Ziyang Zhang
,
Yanwei Wei
,
Zehao Peng
,
Peng Du
,
Xinyong Du
,
Guo‐Ying Zuo
,
Chaoqing Wang
,
Piwu Li
,
Junqing Wang
,
Ruiming Wang
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.3389/fmicb.2023.1160552
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.3389/fmicb.2023.1160552
Sauce-flavor baijiu is one of the twelve flavor types of Chinese distilled fermented product. Microbial composition plays a key role in the stacked fermentation of Baijiu, which uses grains as raw materials and produces flavor compounds, however, the active microbial community and its relationship remain unclear. Here, we investigated the total and active microbial communities of stacked fermented grains of sauce-flavored Baijiu using flow cytometry and high-throughput sequencing technology, respectively. By using traditional high-throughput sequencing technology, a total of 24 bacterial and 14 fungal genera were identified as the core microbiota, the core bacteria were Lactobacillus (0.08–39.05%), Acetobacter (0.25–81.92%), Weissella (0.03–29.61%), etc. The core fungi were Issatchenkia (23.11–98.21%), Monascus (0.02–26.36%), Pichia (0.33–37.56%), etc. In contrast, using flow cytometry combined with high-throughput sequencing, the active dominant bacterial genera after cell sorting were found to be Herbaspirillum , Chitinophaga , Ralstonia , Phenylobacterium , Mucilaginibacter , and Bradyrhizobium , etc., whereas the active dominant fungal genera detected were Aspergillus , Pichia , Exophiala , Candelabrochaete , Italiomyces , and Papiliotrema , etc. These results indicate that although the abundance of Acetobacter , Monascus , and Issatchenkia was high after stacked fermentation, they may have little biological activity. Flow cytometry and cell sorting techniques have been used in the study of beer and wine, but exploring the microbiome in such a complex environment as Chinese baijiu has not been reported. The results also reveal that flow cytometry and cell sorting are convenient methods for rapidly monitoring complex microbial flora and can assist in exploring complex environmental samples.
Related Topics
Concepts
Weissella
Biology
Food science
Monascus
Acetobacter
Fermentation
Flow cytometry
Lactobacillus
Wine
Yeast
Microbiology
Biochemistry
Leuconostoc
Genetics
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3389/fmicb.2023.1160552
- https://www.frontiersin.org/articles/10.3389/fmicb.2023.1160552/pdf
- OA Status
- gold
- Cited By
- 7
- References
- 39
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4360985269
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4360985269Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3389/fmicb.2023.1160552Digital Object Identifier
- Title
-
Exploration of microbiome diversity of stacked fermented grains by flow cytometry and cell sortingWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2023Year of publication
- Publication date
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2023-03-27Full publication date if available
- Authors
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Ziyang Zhang, Yanwei Wei, Zehao Peng, Peng Du, Xinyong Du, Guo‐Ying Zuo, Chaoqing Wang, Piwu Li, Junqing Wang, Ruiming WangList of authors in order
- Landing page
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https://doi.org/10.3389/fmicb.2023.1160552Publisher landing page
- PDF URL
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https://www.frontiersin.org/articles/10.3389/fmicb.2023.1160552/pdfDirect link to full text PDF
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YesWhether a free full text is available
- OA status
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goldOpen access status per OpenAlex
- OA URL
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https://www.frontiersin.org/articles/10.3389/fmicb.2023.1160552/pdfDirect OA link when available
- Concepts
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Weissella, Biology, Food science, Monascus, Acetobacter, Fermentation, Flow cytometry, Lactobacillus, Wine, Yeast, Microbiology, Biochemistry, Leuconostoc, GeneticsTop concepts (fields/topics) attached by OpenAlex
- Cited by
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7Total citation count in OpenAlex
- Citations by year (recent)
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2025: 2, 2024: 2, 2023: 3Per-year citation counts (last 5 years)
- References (count)
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39Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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