Expression of an immunocomplex consisting of Fc fragment fused with a consensus dengue envelope domain III in Saccharomyces cerevisiae Article Swipe
Kum-Kang So
,
Jeesun Chun
,
Nguyễn Ngọc Lương
,
Heewon Seo
,
Dae‐Hyuk Kim
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1007/s10529-021-03161-7
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1007/s10529-021-03161-7
Objectives To explore Saccharomyces cerevisiae as an expression platform for dengue oral immune complex vaccine development. Results Molecular engineering was applied to create a fusion gene construct (scEDIII-PIGS) consisting of a yeast codon optimized sequence encoding for a synthetic consensus dengue envelope domain III (scEDIII) followed by a modified IgG Fc domain (PIGS). Northern blot showed transcription of the target gene, with a temporal expression pattern similar to those from previous work. Western blot showed assembly of various immune complexes from monomer to hexamer. Partial purification of scEDIII-PIGS was also attempted to demonstrate the feasibility of yeast system for immune complex vaccine development. Approximately 1 mg of scEDIII-PIGS can be produced from 1 l culture. Conclusion This work demonstrated for the first time that various immunocomplex structures of our target protein could be efficiently produced in S. cerevisiae for future application in developing oral and injectable vaccines against various pathogens.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1007/s10529-021-03161-7
- https://link.springer.com/content/pdf/10.1007/s10529-021-03161-7.pdf
- OA Status
- hybrid
- Cited By
- 5
- References
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- Related Works
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- OpenAlex ID
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Raw OpenAlex JSON
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- DOI
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https://doi.org/10.1007/s10529-021-03161-7Digital Object Identifier
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Expression of an immunocomplex consisting of Fc fragment fused with a consensus dengue envelope domain III in Saccharomyces cerevisiaeWork title
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articleOpenAlex work type
- Language
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enPrimary language
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2021Year of publication
- Publication date
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2021-07-10Full publication date if available
- Authors
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Kum-Kang So, Jeesun Chun, Nguyễn Ngọc Lương, Heewon Seo, Dae‐Hyuk KimList of authors in order
- Landing page
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https://doi.org/10.1007/s10529-021-03161-7Publisher landing page
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https://link.springer.com/content/pdf/10.1007/s10529-021-03161-7.pdfDirect link to full text PDF
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YesWhether a free full text is available
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hybridOpen access status per OpenAlex
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https://link.springer.com/content/pdf/10.1007/s10529-021-03161-7.pdfDirect OA link when available
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Saccharomyces cerevisiae, Biology, Western blot, Random hexamer, Gene, Immune system, Yeast, Molecular biology, Virology, Biochemistry, GeneticsTop concepts (fields/topics) attached by OpenAlex
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5Total citation count in OpenAlex
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2025: 1, 2024: 3, 2023: 1Per-year citation counts (last 5 years)
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33Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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