Expression of matrix factors in the process of neovascularization of intervertebral disc Article Swipe
Pedro Henrique Isoldi Pohl
,
Thais Cuperman
,
Thomas P. Lozito
,
Takashi Yurube
,
Rocky S. Tuan
,
James D. Kang
,
Nam Vo
,
Luciano Miller Reis Rodrigues
·
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.6084/m9.figshare.20013542
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.6084/m9.figshare.20013542
OBJECTIVE: To investigate the effects of proteins products of endothelial cells (ECs) on the annulus fibrosus (AF) cell metabolism in an in vitro culture.METHODS:Human AF cells were expanded in monolayer cultures and treated with proteins from the medium of cell line HMEC-1 (Human Microvascular Endothelial Cells) (125µg/ml). After 72h of treatment RNA was isolated from AF cells for analysis of gene expression and the culture medium was collected for protein expression analysis.RESULTS: The qRT-PCR analysis demonstrated increased gene expression of matrix metalloproteinases (MMPs) in AF cells treated with protein products of endothelial cells compared with cells from control group of AF cells: MMP-1 243.10 times (p<0.05), MMP-2 1.37 time (p<0.05), MMP-3 39.83 times (p<0.05) and MMP-13 5.70 times (p<0.05). In contrast, tissue inhibitors of metalloproteinases (TIMPs) were suppressed; TIMP-2 (0.55 time) (p<0.05) and TIMP-3 (0.60 time) (p<0.05) in the exposed groups. The expression of aggrecan gene (0.83 time) (p<0.05), an important extracellular matrix component, was also reduced. MMP-1 and MMP-3 detection was performed, confirming the results of PCR by Western Blot technique.CONCLUSIONS: In this study, we observed that the proteins produced by ECs induced the MMPs expression and suppressed the TIMPs as well as the aggrecan in primary cells of the human intervertebral disc, targeting the development of potential treatments for intervertebral disc degeneration and associated discogenic pain.
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https://openalex.org/W4394237876Canonical identifier for this work in OpenAlex
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https://doi.org/10.6084/m9.figshare.20013542Digital Object Identifier
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Expression of matrix factors in the process of neovascularization of intervertebral discWork title
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datasetOpenAlex work type
- Language
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enPrimary language
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2022Year of publication
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2022-01-01Full publication date if available
- Authors
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Pedro Henrique Isoldi Pohl, Thais Cuperman, Thomas P. Lozito, Takashi Yurube, Rocky S. Tuan, James D. Kang, Nam Vo, Luciano Miller Reis RodriguesList of authors in order
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https://doi.org/10.6084/m9.figshare.20013542Publisher landing page
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YesWhether a free full text is available
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goldOpen access status per OpenAlex
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https://doi.org/10.6084/m9.figshare.20013542Direct OA link when available
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Intervertebral disc, Process (computing), Expression (computer science), Matrix (chemical analysis), Computer science, Materials science, Anatomy, Medicine, Composite material, Operating system, Programming languageTop concepts (fields/topics) attached by OpenAlex
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