Extracellular matrix flow guides emergent in-vitro morphogenesis Article Swipe

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Abdel Rahman Abdel Fattah , Francesca Sgualidino , Suresh Poovathingal , Kristofer Davie , Pieter Baatsen , Katlijn Vints , Natalia V. Gounko , Giulio Superti‐Furga , Lorenzo Mattolini , Myriam Roussigné , Bertrand Bénazéraf , Adrian Ranga ·

YOU? · · 2023 · Open Access · · DOI: https://doi.org/10.21203/rs.3.rs-2957065/v1

Morphogenesis of vertebrate embryos requires extracellular matrix (ECM) in multiple developmental contexts¹. Symmetry-breaking events in initially homogenous tissues trigger collective cellular dynamics which follow fluid-like behaviors, and the ECM, which is in constant engagement with morphing tissues, also undergoes fluid-like motion^2–4. Cell and ECM deformations require coordination, however the interplay between them at cellular and tissue-scale remains largely unexplored. Here, we reveal a novel mechanism coupling morphogenetic events and epithelia-driven ECM flow. We show that exposure to ECM components triggers periodic morphogenesis of dome-shaped structures in human pluripotent stem cell monolayers, driven by directional ECM flow towards the sites of morphogenesis. We show that this flow is initiated by local symmetry-breaking events, is driven by microvilli and requires unperturbed flow conditions and cytoskeletal contractility. A theoretical model shows that a reaction-diffusion-like mechanism is responsible for organizing local morphogenesis into global tissue-wide events. The cell patterning landscape predicted by the model is experimentally recapitulated during mesoderm differentiation. These results uncover a new role for the ECM: in addition to being a site of cell adhesion, structural support and mechanical force generation⁵, as is currently established, we show that directional transport of ECM, termed ECM flow, is a major contributor in sustaining epithelial morphogenesis and differentiation.

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Morphogenesis Extracellular matrix Cell biology Biology Cytoskeleton Matrix (chemical analysis) Cell Chemistry Genetics Gene Chromatography

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Type: preprint
Language: en
Landing Page: https://doi.org/10.21203/rs.3.rs-2957065/v1
PDF: https://www.researchsquare.com/article/rs-2957065/latest.pdf
OA Status: gold
References: 49
Related Works: 10
OpenAlex ID: https://openalex.org/W4379987386

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DOI: https://doi.org/10.21203/rs.3.rs-2957065/v1

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Title: Extracellular matrix flow guides emergent in-vitro morphogenesis

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Type: preprint

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Language: en

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Publication year: 2023

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Publication date: 2023-06-09

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Authors: Abdel Rahman Abdel Fattah, Francesca Sgualidino, Suresh Poovathingal, Kristofer Davie, Pieter Baatsen, Katlijn Vints, Natalia V. Gounko, Giulio Superti‐Furga, Lorenzo Mattolini, Myriam Roussigné, Bertrand Bénazéraf, Adrian Ranga

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Landing page: https://doi.org/10.21203/rs.3.rs-2957065/v1

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PDF URL: https://www.researchsquare.com/article/rs-2957065/latest.pdf

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OA URL: https://www.researchsquare.com/article/rs-2957065/latest.pdf

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Concepts: Morphogenesis, Extracellular matrix, Cell biology, Biology, Cytoskeleton, Matrix (chemical analysis), Cell, Chemistry, Genetics, Gene, Chromatography

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abstract_inverted_index.unperturbed	119
abstract_inverted_index.cytoskeletal	123
abstract_inverted_index.deformations	45
abstract_inverted_index.established,	184
abstract_inverted_index.tissue-scale	56
abstract_inverted_index.Morphogenesis	1
abstract_inverted_index.coordination,	47
abstract_inverted_index.developmental	11
abstract_inverted_index.extracellular	6
abstract_inverted_index.morphogenesis	82, 138, 202
abstract_inverted_index.morphogenetic	67
abstract_inverted_index.recapitulated	153
abstract_inverted_index.contractility.	124
abstract_inverted_index.experimentally	152
abstract_inverted_index.morphogenesis.	101
abstract_inverted_index.differentiation.	156, 204
abstract_inverted_index.epithelia-driven	70
abstract_inverted_index.Symmetry-breaking	13
abstract_inverted_index.symmetry-breaking	111
abstract_inverted_index.contexts<sup>1</sup>.	12
abstract_inverted_index.<title>Abstract</title>	0
abstract_inverted_index.generation<sup>5</sup>,	180
abstract_inverted_index.motion<sup>2–4</sup>.	41
abstract_inverted_index.reaction-diffusion-like	131
cited_by_percentile_year
countries_distinct_count	3
institutions_distinct_count	12
sustainable_development_goals[0].id	https://metadata.un.org/sdg/15
sustainable_development_goals[0].score	0.49000000953674316
sustainable_development_goals[0].display_name	Life in Land
citation_normalized_percentile.value	0.25333895
citation_normalized_percentile.is_in_top_1_percent	False
citation_normalized_percentile.is_in_top_10_percent	False