Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents Article Swipe
Miguel P. Batista
,
Naiara Fernández
,
Frédéric Bustos Gaspar
,
Maria Rosário Bronze
,
Ana Rita C. Duarte
·
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.3389/fchem.2022.937036
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.3389/fchem.2022.937036
The disposal of large amounts of skin waste resulting from the blue shark fishing industry presents several industrial and environmental waste management concerns. In addition, these marine subproducts are interesting sources of collagen, a fibrous protein that shows high social and economic interest in a broad range of biomedical, pharmaceutical, and cosmetic applications. However, blue shark wasted skins are a poorly explored matrix for this purpose, and conventional collagen recovery methodologies involve several pre-treatment steps, long extraction times and low temperatures. This work presents a new green and sustainable collagen extraction approach using a natural deep eutectic solvent composed of citric acid:xylitol:water at a 1:1:10 molar ratio, and the chemical characterization of the extracted collagen by discontinuous electrophoresis, thermogravimetric analysis, Fourier transformed infrared spectroscopy and circular dichroism. The extracted material was a pure type I collagen, and the novel approach presented an extraction yield 2.5 times higher than the conventional one, without pre-treatment of raw material and reducing the procedure time from 96 to 1 h. Furthermore, the in vitro cytotoxicity evaluation, performed with a mouse fibroblasts cell line, has proven the biocompatibility of the extracted material. Overall, the obtained results demonstrate a simple, quick, cheap and environmentally sustainable process to obtain marine collagen with promising properties for biomedical and cosmetic applications.
Related Topics
Concepts
Biocompatibility
Extraction (chemistry)
Materials science
Fourier transform infrared spectroscopy
Raw material
Thermogravimetric analysis
Chemical engineering
Pulp and paper industry
Deep eutectic solvent
Eutectic system
Chemistry
Nanotechnology
Chromatography
Organic chemistry
Composite material
Metallurgy
Engineering
Alloy
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3389/fchem.2022.937036
- OA Status
- gold
- Cited By
- 47
- References
- 53
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4283010799
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4283010799Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3389/fchem.2022.937036Digital Object Identifier
- Title
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Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic SolventsWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2022Year of publication
- Publication date
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2022-06-16Full publication date if available
- Authors
-
Miguel P. Batista, Naiara Fernández, Frédéric Bustos Gaspar, Maria Rosário Bronze, Ana Rita C. DuarteList of authors in order
- Landing page
-
https://doi.org/10.3389/fchem.2022.937036Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://doi.org/10.3389/fchem.2022.937036Direct OA link when available
- Concepts
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Biocompatibility, Extraction (chemistry), Materials science, Fourier transform infrared spectroscopy, Raw material, Thermogravimetric analysis, Chemical engineering, Pulp and paper industry, Deep eutectic solvent, Eutectic system, Chemistry, Nanotechnology, Chromatography, Organic chemistry, Composite material, Metallurgy, Engineering, AlloyTop concepts (fields/topics) attached by OpenAlex
- Cited by
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47Total citation count in OpenAlex
- Citations by year (recent)
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2025: 24, 2024: 17, 2023: 6Per-year citation counts (last 5 years)
- References (count)
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53Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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