Factors Affecting the Output Signal of the Electrochemical Immunosensor Based on Chicken Egg Yolk Antibody for Detecting Newcastle Disease Virus Article Swipe
Luyen Thi Tran
,
Huynh Dang Chinh
,
Tran Quang Thinh
,
Mai Anh Tuan
·
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.25073/2588-1140/vnunst.5005
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.25073/2588-1140/vnunst.5005
In this study, the electrochemical immunosensor for detecting Newcastle disease virus using the chicken egg yolk antibodies (IgY) was developed. The developed immunosensor was built into a three-electrode system consisting of two gold electrodes (a working electrode and a counter electrode) and a quasi-reference Ag/AgCl electrode. The IgY antibodies against Newcastle disease virus were immobilized on the working electrode (immunosensor) and the effect of the antibody concentration and virus incubation time on the output signal of the immunosensors was investigated. The investigation results show that when the antibody concentration was 60 µg/mL and the virus incubation time was 1 hour, the output signal of the immunosensors reached its maximum value (∆Ipeak = 0.1602). The detection limit of the sensors was 102 EID50/mL (EID50: 50% Empryo Infective Dose) at 25 oC. There was a good linear relationship between the ∆Ipeak and the logarithm of the virus Newcastle concentration in the range from 102 to 106 EID50/mL. The linear equation is ∆Ipeak = 0.0280logN – 0.00368 with the correlation coefficient of R2 = 0.9972.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.25073/2588-1140/vnunst.5005
- https://js.vnu.edu.vn/NST/article/download/5005/4481
- OA Status
- diamond
- References
- 3
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3117502216
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https://openalex.org/W3117502216Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.25073/2588-1140/vnunst.5005Digital Object Identifier
- Title
-
Factors Affecting the Output Signal of the Electrochemical Immunosensor Based on Chicken Egg Yolk Antibody for Detecting Newcastle Disease VirusWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2020Year of publication
- Publication date
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2020-12-22Full publication date if available
- Authors
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Luyen Thi Tran, Huynh Dang Chinh, Tran Quang Thinh, Mai Anh TuanList of authors in order
- Landing page
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https://doi.org/10.25073/2588-1140/vnunst.5005Publisher landing page
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https://js.vnu.edu.vn/NST/article/download/5005/4481Direct link to full text PDF
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diamondOpen access status per OpenAlex
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https://js.vnu.edu.vn/NST/article/download/5005/4481Direct OA link when available
- Concepts
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Newcastle disease, Yolk, Electrode, Virus, Linear range, Incubation, Antibody, Detection limit, Chromatography, Electrochemistry, Linear relationship, Working electrode, Chemistry, Materials science, Virology, Biology, Biochemistry, Immunology, Mathematics, Food science, Physical chemistry, StatisticsTop concepts (fields/topics) attached by OpenAlex
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0Total citation count in OpenAlex
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10Other works algorithmically related by OpenAlex
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