Fastandlong-term super-resolution imaging of ER nano-structural dynamics in living cells using a neural network Article Swipe
Johanna V. Rahm
,
Ashwin Balakrishnan
,
Maren H. Wehrheim
,
Alexandra Kaminer
,
Marius Glogger
,
Laurell F. Kessler
,
Matthias Kaschube
,
Hans‐Dieter Barth
,
Mike Heilemann
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1101/2024.07.30.605742
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1101/2024.07.30.605742
Stimulated emission depletion (STED) microscopy is a super-resolution technique that surpasses the diffraction limit and has contributed to the study of dynamic processes in living cells. However, high laser intensities induce fluorophore photobleaching and sample phototoxicity, limiting the number of fluorescence images obtainable from a living cell. Here, we address these challenges by using ultra-low irradiation intensities and a neural network for image restoration, enabling extensive imaging of single living cells. The endoplasmic reticulum (ER) was chosen as the target structure due to its dynamic nature over short and long timescales. The reduced irradiation intensity combined with denoising permitted continuous ER dynamics observation in living cells for up to 7 hours with a temporal resolution of seconds. This allowed for quantitative analysis of ER structural features over short (seconds) and long (hours) timescales within the same cell, and enabled fast 3D live-cell STED microscopy. Overall, the combination of ultra-low irradiation with image restoration enables comprehensive analysis of organelle dynamics over extended periods in living cells.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2024.07.30.605742
- https://www.biorxiv.org/content/biorxiv/early/2024/07/30/2024.07.30.605742.full.pdf
- OA Status
- green
- References
- 43
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4401111938
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4401111938Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1101/2024.07.30.605742Digital Object Identifier
- Title
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Fastandlong-term super-resolution imaging of ER nano-structural dynamics in living cells using a neural networkWork title
- Type
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preprintOpenAlex work type
- Language
-
enPrimary language
- Publication year
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2024Year of publication
- Publication date
-
2024-07-30Full publication date if available
- Authors
-
Johanna V. Rahm, Ashwin Balakrishnan, Maren H. Wehrheim, Alexandra Kaminer, Marius Glogger, Laurell F. Kessler, Matthias Kaschube, Hans‐Dieter Barth, Mike HeilemannList of authors in order
- Landing page
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https://doi.org/10.1101/2024.07.30.605742Publisher landing page
- PDF URL
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https://www.biorxiv.org/content/biorxiv/early/2024/07/30/2024.07.30.605742.full.pdfDirect link to full text PDF
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YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
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https://www.biorxiv.org/content/biorxiv/early/2024/07/30/2024.07.30.605742.full.pdfDirect OA link when available
- Concepts
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STED microscopy, Photobleaching, Microscopy, Live cell imaging, Temporal resolution, Endoplasmic reticulum, Irradiation, Dynamics (music), Organelle, Biophysics, Biological system, Chemistry, Fluorescence, Optics, Physics, Stimulated emission, Cell, Cell biology, Laser, Biology, Biochemistry, Acoustics, Nuclear physicsTop concepts (fields/topics) attached by OpenAlex
- Cited by
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0Total citation count in OpenAlex
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43Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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