FIGURE 2 from Development of an Anti-canine PD-L1 Antibody and Caninized PD-L1 Mouse Model as Translational Research Tools for the Study of Immunotherapy in Humans Article Swipe

View

Wonkyung Oh , Alyssa Min Jung Kim , Deepika Dhawan , Perry M. Kirkham , Raluca Ostafe , Jackeline Franco , Uma K. Aryal , Robert H. Carnahan , Valery Patsekin , J. Paul Robinson , Deborah W. Knapp , Seung‐Oe Lim ·

YOU? · · 2023 · Open Access · · DOI: https://doi.org/10.1158/2767-9764.22822019

The high-throughput screening of therapeutic antibodies. A, Schematic diagram of the cPD-L1 antibody binding assay. BT549 cells expressing cPD-L1 were seeded on 96-well or 384-well plates. cPD-L1 antibodies (from hybridomas) and Alexa Fluor 488–conjugated anti-mouse IgG Fc-specific secondary antibody were added, and then green fluorescence signal was measured to quantify the amount of bound PD-L1 antibody by IncuCyte S3. B, A representative result of the cPD-L1 antibody binding assay. Kinetic graphs from each well of a 96-well plate showing quantitative binding of cPD-L1 antibodies on BT549 cells expressing cPD-L1 at 6-hour time intervals. The positive clones are highlighted in red (A2, I6, and I11). C, Representative images (at 18 hours) of cPD-L1 antibody binding. Green fluorescent merged images of cPD-L1–expressing cells are shown. D, Schematic diagram of the cPD-L1/cPD-1 blockade assay. BT549 cells expressing cPD-L1 were seeded on 96-well or 384-well plates. cPD-1-human IgG Fc (hFc) protein, Alexa Fluor 488–conjugated anti-human IgG Fc-specific secondary antibody and/or cPD-L1 antibody were added, and then green fluorescence signal was measured to quantify the amount of bound PD-1 protein by IncuCyte S3. E, A representative result of the cPD-L1/cPD-1 blockade assay. Kinetic graphs from each well of a 96-well plate showing quantitative binding of cPD-1 protein on BT549 cells expressing cPD-L1 at 3-hour intervals after the addition of cPD-L1 antibodies. The positive clones that blocked the interaction of cPD-L1/cPD-1 proteins are highlighted in red (A4 and B8). F, Representative images (at 18 hours) of the cPD-L1/cPD-1 blockade. Green fluorescent merged images of cPD-L1–expressing cells are shown. Note the lack of fluorescence due to the antibody binding to PD-L1 and blocking the interaction with cPD-1.

Related Topics

Concepts

Antibody Molecular biology Chemistry Primary and secondary antibodies Alexa Fluor Fluorescence Biology Immunology Physics Quantum mechanics

Metadata

Type: preprint
Language: en
Landing Page: https://doi.org/10.1158/2767-9764.22822019
OA Status: gold
Related Works: 10
OpenAlex ID: https://openalex.org/W4376617465

All OpenAlex metadata

Raw OpenAlex JSON

OpenAlex ID: https://openalex.org/W4376617465

Canonical identifier for this work in OpenAlex
DOI: https://doi.org/10.1158/2767-9764.22822019

Digital Object Identifier
Title: FIGURE 2 from Development of an Anti-canine PD-L1 Antibody and Caninized PD-L1 Mouse Model as Translational Research Tools for the Study of Immunotherapy in Humans

Work title
Type: preprint

OpenAlex work type
Language: en

Primary language
Publication year: 2023

Year of publication
Publication date: 2023-05-15

Full publication date if available
Authors: Wonkyung Oh, Alyssa Min Jung Kim, Deepika Dhawan, Perry M. Kirkham, Raluca Ostafe, Jackeline Franco, Uma K. Aryal, Robert H. Carnahan, Valery Patsekin, J. Paul Robinson, Deborah W. Knapp, Seung‐Oe Lim

List of authors in order
Landing page: https://doi.org/10.1158/2767-9764.22822019

Publisher landing page
Open access: Yes

Whether a free full text is available
OA status: gold

Open access status per OpenAlex
OA URL: https://doi.org/10.1158/2767-9764.22822019

Direct OA link when available
Concepts: Antibody, Molecular biology, Chemistry, Primary and secondary antibodies, Alexa Fluor, Fluorescence, Biology, Immunology, Physics, Quantum mechanics

Top concepts (fields/topics) attached by OpenAlex
Cited by: 0

Total citation count in OpenAlex
Related works (count): 10

Other works algorithmically related by OpenAlex

Full payload

id	https://openalex.org/W4376617465
doi	https://doi.org/10.1158/2767-9764.22822019
ids.doi	https://doi.org/10.1158/2767-9764.22822019
ids.openalex	https://openalex.org/W4376617465
fwci	0.0
type	preprint
title	FIGURE 2 from Development of an Anti-canine PD-L1 Antibody and Caninized PD-L1 Mouse Model as Translational Research Tools for the Study of Immunotherapy in Humans
biblio.issue
biblio.volume
biblio.last_page
biblio.first_page
topics[0].id	https://openalex.org/T12996
topics[0].field.id	https://openalex.org/fields/13
topics[0].field.display_name	Biochemistry, Genetics and Molecular Biology
topics[0].score	0.9807000160217285
topics[0].domain.id	https://openalex.org/domains/1
topics[0].domain.display_name	Life Sciences
topics[0].subfield.id	https://openalex.org/subfields/1305
topics[0].subfield.display_name	Biotechnology
topics[0].display_name	Cancer Research and Treatments
topics[1].id	https://openalex.org/T11016
topics[1].field.id	https://openalex.org/fields/27
topics[1].field.display_name	Medicine
topics[1].score	0.9775000214576721
topics[1].domain.id	https://openalex.org/domains/4
topics[1].domain.display_name	Health Sciences
topics[1].subfield.id	https://openalex.org/subfields/2741
topics[1].subfield.display_name	Radiology, Nuclear Medicine and Imaging
topics[1].display_name	Monoclonal and Polyclonal Antibodies Research
topics[2].id	https://openalex.org/T10580
topics[2].field.id	https://openalex.org/fields/24
topics[2].field.display_name	Immunology and Microbiology
topics[2].score	0.9733999967575073
topics[2].domain.id	https://openalex.org/domains/1
topics[2].domain.display_name	Life Sciences
topics[2].subfield.id	https://openalex.org/subfields/2403
topics[2].subfield.display_name	Immunology
topics[2].display_name	Immunotherapy and Immune Responses
is_xpac	False
apc_list
apc_paid
concepts[0].id	https://openalex.org/C159654299
concepts[0].level	2
concepts[0].score	0.7872291803359985
concepts[0].wikidata	https://www.wikidata.org/wiki/Q79460
concepts[0].display_name	Antibody
concepts[1].id	https://openalex.org/C153911025
concepts[1].level	1
concepts[1].score	0.6259051561355591
concepts[1].wikidata	https://www.wikidata.org/wiki/Q7202
concepts[1].display_name	Molecular biology
concepts[2].id	https://openalex.org/C185592680
concepts[2].level	0
concepts[2].score	0.4858534038066864
concepts[2].wikidata	https://www.wikidata.org/wiki/Q2329
concepts[2].display_name	Chemistry
concepts[3].id	https://openalex.org/C21269302
concepts[3].level	3
concepts[3].score	0.457967072725296
concepts[3].wikidata	https://www.wikidata.org/wiki/Q7243098
concepts[3].display_name	Primary and secondary antibodies
concepts[4].id	https://openalex.org/C2776759294
concepts[4].level	3
concepts[4].score	0.4384789764881134
concepts[4].wikidata	https://www.wikidata.org/wiki/Q2833068
concepts[4].display_name	Alexa Fluor
concepts[5].id	https://openalex.org/C91881484
concepts[5].level	2
concepts[5].score	0.43272554874420166
concepts[5].wikidata	https://www.wikidata.org/wiki/Q191807
concepts[5].display_name	Fluorescence
concepts[6].id	https://openalex.org/C86803240
concepts[6].level	0
concepts[6].score	0.33921343088150024
concepts[6].wikidata	https://www.wikidata.org/wiki/Q420
concepts[6].display_name	Biology
concepts[7].id	https://openalex.org/C203014093
concepts[7].level	1
concepts[7].score	0.22829541563987732
concepts[7].wikidata	https://www.wikidata.org/wiki/Q101929
concepts[7].display_name	Immunology
concepts[8].id	https://openalex.org/C121332964
concepts[8].level	0
concepts[8].score	0.08002352714538574
concepts[8].wikidata	https://www.wikidata.org/wiki/Q413
concepts[8].display_name	Physics
concepts[9].id	https://openalex.org/C62520636
concepts[9].level	1
concepts[9].score	0.0
concepts[9].wikidata	https://www.wikidata.org/wiki/Q944
concepts[9].display_name	Quantum mechanics
keywords[0].id	https://openalex.org/keywords/antibody
keywords[0].score	0.7872291803359985
keywords[0].display_name	Antibody
keywords[1].id	https://openalex.org/keywords/molecular-biology
keywords[1].score	0.6259051561355591
keywords[1].display_name	Molecular biology
keywords[2].id	https://openalex.org/keywords/chemistry
keywords[2].score	0.4858534038066864
keywords[2].display_name	Chemistry
keywords[3].id	https://openalex.org/keywords/primary-and-secondary-antibodies
keywords[3].score	0.457967072725296
keywords[3].display_name	Primary and secondary antibodies
keywords[4].id	https://openalex.org/keywords/alexa-fluor
keywords[4].score	0.4384789764881134
keywords[4].display_name	Alexa Fluor
keywords[5].id	https://openalex.org/keywords/fluorescence
keywords[5].score	0.43272554874420166
keywords[5].display_name	Fluorescence
keywords[6].id	https://openalex.org/keywords/biology
keywords[6].score	0.33921343088150024
keywords[6].display_name	Biology
keywords[7].id	https://openalex.org/keywords/immunology
keywords[7].score	0.22829541563987732
keywords[7].display_name	Immunology
keywords[8].id	https://openalex.org/keywords/physics
keywords[8].score	0.08002352714538574
keywords[8].display_name	Physics
language	en
locations[0].id	doi:10.1158/2767-9764.22822019
locations[0].is_oa	True
locations[0].source
locations[0].license	cc-by
locations[0].pdf_url
locations[0].version	acceptedVersion
locations[0].raw_type	posted-content
locations[0].license_id	https://openalex.org/licenses/cc-by
locations[0].is_accepted	True
locations[0].is_published	False
locations[0].raw_source_name
locations[0].landing_page_url	https://doi.org/10.1158/2767-9764.22822019
indexed_in	crossref
authorships[0].author.id	https://openalex.org/A5063154413
authorships[0].author.orcid	https://orcid.org/0009-0003-8005-1085
authorships[0].author.display_name	Wonkyung Oh
authorships[0].author_position	first
authorships[0].raw_author_name	Wonkyung Oh
authorships[0].is_corresponding	False
authorships[1].author.id	https://openalex.org/A5040624132
authorships[1].author.orcid	https://orcid.org/0009-0007-4196-4016
authorships[1].author.display_name	Alyssa Min Jung Kim
authorships[1].author_position	middle
authorships[1].raw_author_name	Alyssa Min Jung Kim
authorships[1].is_corresponding	False
authorships[2].author.id	https://openalex.org/A5019247175
authorships[2].author.orcid	https://orcid.org/0000-0002-5456-9302
authorships[2].author.display_name	Deepika Dhawan
authorships[2].author_position	middle
authorships[2].raw_author_name	Deepika Dhawan
authorships[2].is_corresponding	False
authorships[3].author.id	https://openalex.org/A5086441141
authorships[3].author.orcid	https://orcid.org/0009-0000-8147-6411
authorships[3].author.display_name	Perry M. Kirkham
authorships[3].author_position	middle
authorships[3].raw_author_name	Perry M. Kirkham
authorships[3].is_corresponding	False
authorships[4].author.id	https://openalex.org/A5013268038
authorships[4].author.orcid	https://orcid.org/0000-0003-0479-1527
authorships[4].author.display_name	Raluca Ostafe
authorships[4].author_position	middle
authorships[4].raw_author_name	Raluca Ostafe
authorships[4].is_corresponding	False
authorships[5].author.id	https://openalex.org/A5033496470
authorships[5].author.orcid	https://orcid.org/0000-0002-0737-4764
authorships[5].author.display_name	Jackeline Franco
authorships[5].author_position	middle
authorships[5].raw_author_name	Jackeline Franco
authorships[5].is_corresponding	False
authorships[6].author.id	https://openalex.org/A5059830381
authorships[6].author.orcid	https://orcid.org/0000-0003-4543-1536
authorships[6].author.display_name	Uma K. Aryal
authorships[6].author_position	middle
authorships[6].raw_author_name	Uma K. Aryal
authorships[6].is_corresponding	False
authorships[7].author.id	https://openalex.org/A5015712888
authorships[7].author.orcid	https://orcid.org/0000-0001-5230-1532
authorships[7].author.display_name	Robert H. Carnahan
authorships[7].author_position	middle
authorships[7].raw_author_name	Robert H. Carnahan
authorships[7].is_corresponding	False
authorships[8].author.id	https://openalex.org/A5026115858
authorships[8].author.orcid	https://orcid.org/0000-0001-9314-2582
authorships[8].author.display_name	Valery Patsekin
authorships[8].author_position	middle
authorships[8].raw_author_name	Valery Patsekin
authorships[8].is_corresponding	False
authorships[9].author.id	https://openalex.org/A5053912700
authorships[9].author.orcid	https://orcid.org/0000-0001-8383-3240
authorships[9].author.display_name	J. Paul Robinson
authorships[9].author_position	middle
authorships[9].raw_author_name	J. Paul Robinson
authorships[9].is_corresponding	False
authorships[10].author.id	https://openalex.org/A5048434031
authorships[10].author.orcid	https://orcid.org/0000-0002-0059-0202
authorships[10].author.display_name	Deborah W. Knapp
authorships[10].author_position	middle
authorships[10].raw_author_name	Deborah W. Knapp
authorships[10].is_corresponding	False
authorships[11].author.id	https://openalex.org/A5046512553
authorships[11].author.orcid	https://orcid.org/0000-0002-8138-4549
authorships[11].author.display_name	Seung‐Oe Lim
authorships[11].author_position	last
authorships[11].raw_author_name	Seung-Oe Lim
authorships[11].is_corresponding	False
has_content.pdf	False
has_content.grobid_xml	False
is_paratext	False
open_access.is_oa	True
open_access.oa_url	https://doi.org/10.1158/2767-9764.22822019
open_access.oa_status	gold
open_access.any_repository_has_fulltext	False
created_date	2025-10-10T00:00:00
display_name	FIGURE 2 from Development of an Anti-canine PD-L1 Antibody and Caninized PD-L1 Mouse Model as Translational Research Tools for the Study of Immunotherapy in Humans
has_fulltext	False
is_retracted	False
updated_date	2025-11-06T03:46:38.306776
primary_topic.id	https://openalex.org/T12996
primary_topic.field.id	https://openalex.org/fields/13
primary_topic.field.display_name	Biochemistry, Genetics and Molecular Biology
primary_topic.score	0.9807000160217285
primary_topic.domain.id	https://openalex.org/domains/1
primary_topic.domain.display_name	Life Sciences
primary_topic.subfield.id	https://openalex.org/subfields/1305
primary_topic.subfield.display_name	Biotechnology
primary_topic.display_name	Cancer Research and Treatments
related_works	https://openalex.org/W2074123101, https://openalex.org/W4234265326, https://openalex.org/W4248602512, https://openalex.org/W4206239103, https://openalex.org/W1515544699, https://openalex.org/W4240757592, https://openalex.org/W4229905528, https://openalex.org/W199449785, https://openalex.org/W2279591815, https://openalex.org/W1578175851
cited_by_count	0
locations_count	1
best_oa_location.id	doi:10.1158/2767-9764.22822019
best_oa_location.is_oa	True
best_oa_location.source
best_oa_location.license	cc-by
best_oa_location.pdf_url
best_oa_location.version	acceptedVersion
best_oa_location.raw_type	posted-content
best_oa_location.license_id	https://openalex.org/licenses/cc-by
best_oa_location.is_accepted	True
best_oa_location.is_published	False
best_oa_location.raw_source_name
best_oa_location.landing_page_url	https://doi.org/10.1158/2767-9764.22822019
primary_location.id	doi:10.1158/2767-9764.22822019
primary_location.is_oa	True
primary_location.source
primary_location.license	cc-by
primary_location.pdf_url
primary_location.version	acceptedVersion
primary_location.raw_type	posted-content
primary_location.license_id	https://openalex.org/licenses/cc-by
primary_location.is_accepted	True
primary_location.is_published	False
primary_location.raw_source_name
primary_location.landing_page_url	https://doi.org/10.1158/2767-9764.22822019
publication_date	2023-05-15
publication_year	2023
referenced_works_count	0
abstract_inverted_index.A	60, 179
abstract_inverted_index.a	75, 193
abstract_inverted_index.18	108, 237
abstract_inverted_index.Fc	144
abstract_inverted_index.at	89, 207
abstract_inverted_index.by	56, 175
abstract_inverted_index.in	98, 228
abstract_inverted_index.of	3, 9, 52, 63, 74, 81, 110, 118, 126, 171, 182, 192, 199, 213, 223, 239, 247, 255
abstract_inverted_index.on	21, 84, 137, 202
abstract_inverted_index.or	23, 139
abstract_inverted_index.to	48, 167, 258, 262
abstract_inverted_index.(A4	230
abstract_inverted_index.(at	107, 236
abstract_inverted_index.I6,	101
abstract_inverted_index.IgG	35, 143, 151
abstract_inverted_index.S3.	58, 177
abstract_inverted_index.The	93, 216
abstract_inverted_index.and	30, 41, 102, 160, 231, 264
abstract_inverted_index.are	96, 121, 226, 250
abstract_inverted_index.due	257
abstract_inverted_index.red	99, 229
abstract_inverted_index.the	10, 50, 64, 127, 169, 183, 211, 221, 240, 253, 259, 266
abstract_inverted_index.was	46, 165
abstract_inverted_index.(A2,	100
abstract_inverted_index.B8).	232
abstract_inverted_index.Note	252
abstract_inverted_index.PD-1	173
abstract_inverted_index.each	72, 190
abstract_inverted_index.from	71, 189
abstract_inverted_index.lack	254
abstract_inverted_index.that	219
abstract_inverted_index.then	42, 161
abstract_inverted_index.time	91
abstract_inverted_index.well	73, 191
abstract_inverted_index.were	19, 39, 135, 158
abstract_inverted_index.with	268
abstract_inverted_index.(from	28
abstract_inverted_index.(hFc)	145
abstract_inverted_index.Alexa	31, 147
abstract_inverted_index.BT549	15, 85, 131, 203
abstract_inverted_index.Fluor	32, 148
abstract_inverted_index.Green	114, 243
abstract_inverted_index.I11).	103
abstract_inverted_index.PD-L1	54, 263
abstract_inverted_index.after	210
abstract_inverted_index.bound	53, 172
abstract_inverted_index.cPD-1	200
abstract_inverted_index.cells	16, 86, 120, 132, 204, 249
abstract_inverted_index.green	43, 162
abstract_inverted_index.plate	77, 195
abstract_inverted_index.3-hour	208
abstract_inverted_index.6-hour	90
abstract_inverted_index.added,	40, 159
abstract_inverted_index.amount	51, 170
abstract_inverted_index.and/or	155
abstract_inverted_index.assay.	14, 68, 130, 186
abstract_inverted_index.cPD-L1	11, 18, 26, 65, 82, 88, 111, 134, 156, 206, 214
abstract_inverted_index.clones	95, 218
abstract_inverted_index.graphs	70, 188
abstract_inverted_index.hours)	109, 238
abstract_inverted_index.images	106, 117, 235, 246
abstract_inverted_index.merged	116, 245
abstract_inverted_index.result	62, 181
abstract_inverted_index.seeded	20, 136
abstract_inverted_index.shown.	122, 251
abstract_inverted_index.signal	45, 164
abstract_inverted_index.96-well	22, 76, 138, 194
abstract_inverted_index.Kinetic	69, 187
abstract_inverted_index.binding	13, 67, 80, 198, 261
abstract_inverted_index.blocked	220
abstract_inverted_index.diagram	8, 125
abstract_inverted_index.plates.	25, 141
abstract_inverted_index.protein	174, 201
abstract_inverted_index.showing	78, 196
abstract_inverted_index.384-well	24, 140
abstract_inverted_index.IncuCyte	57, 176
abstract_inverted_index.addition	212
abstract_inverted_index.antibody	12, 38, 55, 66, 112, 154, 157, 260
abstract_inverted_index.binding.	113
abstract_inverted_index.blockade	129, 185
abstract_inverted_index.blocking	265
abstract_inverted_index.measured	47, 166
abstract_inverted_index.positive	94, 217
abstract_inverted_index.protein,	146
abstract_inverted_index.proteins	225
abstract_inverted_index.quantify	49, 168
abstract_inverted_index.Schematic	7, 124
abstract_inverted_index.blockade.	242
abstract_inverted_index.intervals	209
abstract_inverted_index.screening	2
abstract_inverted_index.secondary	37, 153
abstract_inverted_index.anti-human	150
abstract_inverted_index.anti-mouse	34
abstract_inverted_index.antibodies	27, 83
abstract_inverted_index.expressing	17, 87, 133, 205
abstract_inverted_index.intervals.	92
abstract_inverted_index.Fc-specific	36, 152
abstract_inverted_index.antibodies.	5, 215
abstract_inverted_index.cPD-1-human	142
abstract_inverted_index.fluorescent	115, 244
abstract_inverted_index.highlighted	97, 227
abstract_inverted_index.hybridomas)	29
abstract_inverted_index.interaction	222, 267
abstract_inverted_index.therapeutic	4
abstract_inverted_index.<p>The	0
abstract_inverted_index.cPD-L1/cPD-1	128, 184, 224, 241
abstract_inverted_index.fluorescence	44, 163, 256
abstract_inverted_index.quantitative	79, 197
abstract_inverted_index.Representative	105, 234
abstract_inverted_index.representative	61, 180
abstract_inverted_index.high-throughput	1
abstract_inverted_index.488–conjugated	33, 149
abstract_inverted_index.cPD-1.</p>	269
abstract_inverted_index.cPD-L1–expressing	119, 248
abstract_inverted_index.<b>A,</b>	6
abstract_inverted_index.<b>B,</b>	59
abstract_inverted_index.<b>C,</b>	104
abstract_inverted_index.<b>D,</b>	123
abstract_inverted_index.<b>E,</b>	178
abstract_inverted_index.<b>F,</b>	233
cited_by_percentile_year
countries_distinct_count	0
institutions_distinct_count	12
citation_normalized_percentile.value	0.28948783
citation_normalized_percentile.is_in_top_1_percent	False
citation_normalized_percentile.is_in_top_10_percent	False