Figure S10 from Palmitic Acid Accumulation Activates Fibroblasts and Promotes Matrix Stiffness in Colorectal Cancer Article Swipe

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Colorectal cancer Palmitic acid Stiffness Matrix (chemical analysis) Cancer research Medicine Chemistry Materials science Internal medicine Cancer Composite material Biochemistry Fatty acid

Shenghe Deng , Jun Wang , Falong Zou , Denglong Cheng , Mian Chen , Junnan Gu , Jianguo Shi , Jia Yang , Yifan Xue , Zhenxin Jiang , Le Qin , Fuwei Mao , Xiaona Chang , Xiu Nie , Li Liu , Yinghao Cao , Kailin Cai ·

YOU? · · 2025 · Open Access · · DOI: https://doi.org/10.1158/0008-5472.29071878 · OA: W4410403460

<p>Figure S10: PA does not directly promote NF-to-mCAF activation. (A) CCK-8 assay determining the optimal concentration of CAFs stimulated by PA. (B) CCK-8 assay analysis of the relationship between PA stimulation and CAFs proliferation. (C) Evaluation of changes in MMPs and TIMPs secretion in CAFs and NFs induced by PA. (D) qPCR assay was conducted to examine whether PA stimulation can promote gene expression of FN1, POSTN, and LUM (mCAFs maker) in CAFs. (E-F) Western blotting (E) and CCK-8 (F) assays confirmed whether PA could directly promote the activation of NFs to CAFs and facilitates NFs proliferation. (G) qPCR assay examined whether PA stimulation can promote mCAFs marker (FN1, POSTN, and LUM) gene expression. Statistical significance was determined by one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, no significance.</p>