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Matheus Henrique Dias , Anoek Friskes , Siying Wang , João M. Fernandes Neto , Frank van Gemert , Soufiane Mourragui , Chrysa Papagianni , Hendrik J. Kuiken , Sara Mainardi , Daniel Álvarez‐Villanueva , Cor Lieftink , Ben Morris , Anna Dekker , Emma van Dijk , Lieke H.S. Wilms , Marcelo S. da Silva , Robin A. Jansen , Antonio Mulero‐Sánchez , Elke Malzer , August Vidal , Cristina Santos , Ramón Salazar , Rosangela A.M. Wailemann , Thompson E.P. Torres , Giulia De Conti , Jonne A. Raaijmakers , Pétur Snæbjörnsson , Shengxian Yuan , Wenxin Qin , John S. Kovach , Hugo A. Armelin , Hein te Riele , Alexander van Oudenaarden , Haojie Jin , Roderick L. Beijersbergen , Alberto Villanueva , René H. Medema , René Bernards ·

YOU? · · 2024 · Open Access · · DOI: https://doi.org/10.1158/2159-8290.26134858.v1

Figure S3: High N-Myc levels sensitize neuroblastoma cells to LB-100, and PP2A knockdown sensitizes CRC cells to WEE1 inhibition (A) Western blots comparing N-Myc levels in isogenic neuroblastoma models. GAPDH was used as a loading control. (B) long-term viability assays compares the toxicity of LB-100 across these neuroblastoma models. LB-100 was refreshed every 2-3 days, and the cells were grown for 10 days before fixing, staining, and imaging. (C) Western blots show the knockdown of PPP2R1A in HT-29 and SW-480 cells. GAPDH was used as a loading control. (D) Dose-response assays show the effect of Adavosertib after PPP2R1A knockdown compared to a control shRNA in HT-29 and SW-480 cells. Cell viability was estimated by resazurin fluorescence after 5 days in the presence of the drug or DMSO control. The normalized values are plotted. (E) The heat map shows all “Hallmarks” and “KEGG” molecular signatures significantly enriched by LB-100 (4 µM), calyculin A (5 nM), or okadaic acid (10 nM) in HT-29 and SW-480 cells after 8 hours. Asterisks indicate significance level (* p-value <0.05, ** p-value <0.01, *** p-value <0.001).

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Cancer

Medicine

Internal Medicine

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Cancer Cancer research Psychology Medicine Internal medicine

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Type: preprint
Language: en
Landing Page: https://doi.org/10.1158/2159-8290.26134858.v1
OA Status: gold
Related Works: 10
OpenAlex ID: https://openalex.org/W4400214423

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Title: Figure S3 from Paradoxical Activation of Oncogenic Signaling as a Cancer Treatment Strategy

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Authors: Matheus Henrique Dias, Anoek Friskes, Siying Wang, João M. Fernandes Neto, Frank van Gemert, Soufiane Mourragui, Chrysa Papagianni, Hendrik J. Kuiken, Sara Mainardi, Daniel Álvarez‐Villanueva, Cor Lieftink, Ben Morris, Anna Dekker, Emma van Dijk, Lieke H.S. Wilms, Marcelo S. da Silva, Robin A. Jansen, Antonio Mulero‐Sánchez, Elke Malzer, August Vidal, Cristina Santos, Ramón Salazar, Rosangela A.M. Wailemann, Thompson E.P. Torres, Giulia De Conti, Jonne A. Raaijmakers, Pétur Snæbjörnsson, Shengxian Yuan, Wenxin Qin, John S. Kovach, Hugo A. Armelin, Hein te Riele, Alexander van Oudenaarden, Haojie Jin, Roderick L. Beijersbergen, Alberto Villanueva, René H. Medema, René Bernards

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Concepts: Cancer, Cancer research, Psychology, Medicine, Internal medicine

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