Flow-cytometric cell sorting coupled with UV mutagenesis for improving pectin lyase expression Article Swipe
Ke Fang
,
Jun Ma
,
Xinyu Wang
,
Ziting Xu
,
Ziyang Zhang
,
Piwu Li
,
Ruiming Wang
,
Junqing Wang
,
Chuying Sun
,
Ziyang Dong
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.3389/fbioe.2023.1251342
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.3389/fbioe.2023.1251342
Introduction: Alkaline pectin lyase is an important enzyme with a wide range of applications in industrial production, It has been widely used in many important fields such as fruit juice processing and extraction, the dyeing and processing of cotton and linen textiles, degumming plant fibers, environmental industrial wastewater treatment, and pulp and paper production. PGLA-rep4 was previously generated as a modified alkaline pectin lyase with high specific activity at pH 11.0°C and 70°C. However, the pre-constructed high-activity pectin lyase expression strains are still difficult to apply in industrial production due to their limited enzymatic activity. We hope to solve these problems by combining modern breeding techniques with high-throughput equipment to rapidly screen alkaline pectin lyase with higher enzymatic activity and lower cost. Methods: We fused the genes encoding PGLA-rep4 and fluorescent protein egfp using a flexible linker peptide and ligated them into a temperature - sensitive plasmid, pKD46. The constructed screening plasmid pKD46-PGLA-rep4- egfp was then transformed into an expression host and screened via flow-cytometric cell sorting coupled with UV mutagenesis. Results: Following mutagenesis, primary screening, and secondary screening, the high-expression strain, named Escherichia coli BL21/1G3, was obtained. The screening plasmid pKD46-PGLA-rep4- egfp was eliminated, and the original expression plasmid pET28a-PGLA-rep4 was then retransformed into the mutant strains. After induction and fermentation, pectin lyase activity in E. coli BL21/1G3 was significantly increased (1.37-fold relative to that in the parental E. coli BL21/PGLA-rep4 strain, p < 0.001), and the highest activity was 230, 240 U/mL at 144 h. Genome sequencing revealed that genes encoding ribonuclease E (RNase E) and diadenosine tetraphosphatase (ApaH) of E. coli BL21/1G3 were mutated compared to the sequence in the original E. coli BL21 (DE3) strain, which could be associated with increased enzyme expression. Discussion: Our work provides an effective method for the construction of strains expressing pectin lyase at high levels.
Related Topics
Concepts
Pectin
Pectate lyase
Plasmid
Pectin lyase
Biochemistry
Green fluorescent protein
Escherichia coli
Mutagenesis
Fermentation
Enzyme
Mutant
Biology
Chemistry
Gene
Pectinase
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3389/fbioe.2023.1251342
- https://www.frontiersin.org/articles/10.3389/fbioe.2023.1251342/pdf
- OA Status
- gold
- Cited By
- 5
- References
- 43
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4386316133
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W4386316133Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.3389/fbioe.2023.1251342Digital Object Identifier
- Title
-
Flow-cytometric cell sorting coupled with UV mutagenesis for improving pectin lyase expressionWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2023Year of publication
- Publication date
-
2023-08-31Full publication date if available
- Authors
-
Ke Fang, Jun Ma, Xinyu Wang, Ziting Xu, Ziyang Zhang, Piwu Li, Ruiming Wang, Junqing Wang, Chuying Sun, Ziyang DongList of authors in order
- Landing page
-
https://doi.org/10.3389/fbioe.2023.1251342Publisher landing page
- PDF URL
-
https://www.frontiersin.org/articles/10.3389/fbioe.2023.1251342/pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://www.frontiersin.org/articles/10.3389/fbioe.2023.1251342/pdfDirect OA link when available
- Concepts
-
Pectin, Pectate lyase, Plasmid, Pectin lyase, Biochemistry, Green fluorescent protein, Escherichia coli, Mutagenesis, Fermentation, Enzyme, Mutant, Biology, Chemistry, Gene, PectinaseTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
5Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 4, 2024: 1Per-year citation counts (last 5 years)
- References (count)
-
43Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W4386316133 |
|---|---|
| doi | https://doi.org/10.3389/fbioe.2023.1251342 |
| ids.doi | https://doi.org/10.3389/fbioe.2023.1251342 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/37720319 |
| ids.openalex | https://openalex.org/W4386316133 |
| fwci | 1.32090116 |
| type | article |
| title | Flow-cytometric cell sorting coupled with UV mutagenesis for improving pectin lyase expression |
| biblio.issue | |
| biblio.volume | 11 |
| biblio.last_page | 1251342 |
| biblio.first_page | 1251342 |
| topics[0].id | https://openalex.org/T10869 |
| topics[0].field.id | https://openalex.org/fields/11 |
| topics[0].field.display_name | Agricultural and Biological Sciences |
| topics[0].score | 0.9965000152587891 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1110 |
| topics[0].subfield.display_name | Plant Science |
| topics[0].display_name | Polysaccharides and Plant Cell Walls |
| topics[1].id | https://openalex.org/T10240 |
| topics[1].field.id | https://openalex.org/fields/13 |
| topics[1].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[1].score | 0.9927999973297119 |
| topics[1].domain.id | https://openalex.org/domains/1 |
| topics[1].domain.display_name | Life Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/1312 |
| topics[1].subfield.display_name | Molecular Biology |
| topics[1].display_name | Plant tissue culture and regeneration |
| topics[2].id | https://openalex.org/T10171 |
| topics[2].field.id | https://openalex.org/fields/22 |
| topics[2].field.display_name | Engineering |
| topics[2].score | 0.9821000099182129 |
| topics[2].domain.id | https://openalex.org/domains/3 |
| topics[2].domain.display_name | Physical Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/2204 |
| topics[2].subfield.display_name | Biomedical Engineering |
| topics[2].display_name | Biofuel production and bioconversion |
| is_xpac | False |
| apc_list.value | 2950 |
| apc_list.currency | USD |
| apc_list.value_usd | 2950 |
| apc_paid.value | 2950 |
| apc_paid.currency | USD |
| apc_paid.value_usd | 2950 |
| concepts[0].id | https://openalex.org/C2780756937 |
| concepts[0].level | 2 |
| concepts[0].score | 0.7340575456619263 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q188154 |
| concepts[0].display_name | Pectin |
| concepts[1].id | https://openalex.org/C2776681119 |
| concepts[1].level | 4 |
| concepts[1].score | 0.6621835827827454 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q24776974 |
| concepts[1].display_name | Pectate lyase |
| concepts[2].id | https://openalex.org/C22744801 |
| concepts[2].level | 3 |
| concepts[2].score | 0.642398476600647 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q172778 |
| concepts[2].display_name | Plasmid |
| concepts[3].id | https://openalex.org/C2777191336 |
| concepts[3].level | 4 |
| concepts[3].score | 0.6371229887008667 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q9057274 |
| concepts[3].display_name | Pectin lyase |
| concepts[4].id | https://openalex.org/C55493867 |
| concepts[4].level | 1 |
| concepts[4].score | 0.5363655686378479 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q7094 |
| concepts[4].display_name | Biochemistry |
| concepts[5].id | https://openalex.org/C142613039 |
| concepts[5].level | 3 |
| concepts[5].score | 0.5196924805641174 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q272983 |
| concepts[5].display_name | Green fluorescent protein |
| concepts[6].id | https://openalex.org/C547475151 |
| concepts[6].level | 3 |
| concepts[6].score | 0.5092874765396118 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q25419 |
| concepts[6].display_name | Escherichia coli |
| concepts[7].id | https://openalex.org/C16318435 |
| concepts[7].level | 4 |
| concepts[7].score | 0.50834721326828 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q149299 |
| concepts[7].display_name | Mutagenesis |
| concepts[8].id | https://openalex.org/C100544194 |
| concepts[8].level | 2 |
| concepts[8].score | 0.4876321852207184 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q41760 |
| concepts[8].display_name | Fermentation |
| concepts[9].id | https://openalex.org/C181199279 |
| concepts[9].level | 2 |
| concepts[9].score | 0.4736909866333008 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q8047 |
| concepts[9].display_name | Enzyme |
| concepts[10].id | https://openalex.org/C143065580 |
| concepts[10].level | 3 |
| concepts[10].score | 0.46036282181739807 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q3285695 |
| concepts[10].display_name | Mutant |
| concepts[11].id | https://openalex.org/C86803240 |
| concepts[11].level | 0 |
| concepts[11].score | 0.4144342839717865 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[11].display_name | Biology |
| concepts[12].id | https://openalex.org/C185592680 |
| concepts[12].level | 0 |
| concepts[12].score | 0.39863321185112 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q2329 |
| concepts[12].display_name | Chemistry |
| concepts[13].id | https://openalex.org/C104317684 |
| concepts[13].level | 2 |
| concepts[13].score | 0.19976481795310974 |
| concepts[13].wikidata | https://www.wikidata.org/wiki/Q7187 |
| concepts[13].display_name | Gene |
| concepts[14].id | https://openalex.org/C172854840 |
| concepts[14].level | 3 |
| concepts[14].score | 0.13634911179542542 |
| concepts[14].wikidata | https://www.wikidata.org/wiki/Q419568 |
| concepts[14].display_name | Pectinase |
| keywords[0].id | https://openalex.org/keywords/pectin |
| keywords[0].score | 0.7340575456619263 |
| keywords[0].display_name | Pectin |
| keywords[1].id | https://openalex.org/keywords/pectate-lyase |
| keywords[1].score | 0.6621835827827454 |
| keywords[1].display_name | Pectate lyase |
| keywords[2].id | https://openalex.org/keywords/plasmid |
| keywords[2].score | 0.642398476600647 |
| keywords[2].display_name | Plasmid |
| keywords[3].id | https://openalex.org/keywords/pectin-lyase |
| keywords[3].score | 0.6371229887008667 |
| keywords[3].display_name | Pectin lyase |
| keywords[4].id | https://openalex.org/keywords/biochemistry |
| keywords[4].score | 0.5363655686378479 |
| keywords[4].display_name | Biochemistry |
| keywords[5].id | https://openalex.org/keywords/green-fluorescent-protein |
| keywords[5].score | 0.5196924805641174 |
| keywords[5].display_name | Green fluorescent protein |
| keywords[6].id | https://openalex.org/keywords/escherichia-coli |
| keywords[6].score | 0.5092874765396118 |
| keywords[6].display_name | Escherichia coli |
| keywords[7].id | https://openalex.org/keywords/mutagenesis |
| keywords[7].score | 0.50834721326828 |
| keywords[7].display_name | Mutagenesis |
| keywords[8].id | https://openalex.org/keywords/fermentation |
| keywords[8].score | 0.4876321852207184 |
| keywords[8].display_name | Fermentation |
| keywords[9].id | https://openalex.org/keywords/enzyme |
| keywords[9].score | 0.4736909866333008 |
| keywords[9].display_name | Enzyme |
| keywords[10].id | https://openalex.org/keywords/mutant |
| keywords[10].score | 0.46036282181739807 |
| keywords[10].display_name | Mutant |
| keywords[11].id | https://openalex.org/keywords/biology |
| keywords[11].score | 0.4144342839717865 |
| keywords[11].display_name | Biology |
| keywords[12].id | https://openalex.org/keywords/chemistry |
| keywords[12].score | 0.39863321185112 |
| keywords[12].display_name | Chemistry |
| keywords[13].id | https://openalex.org/keywords/gene |
| keywords[13].score | 0.19976481795310974 |
| keywords[13].display_name | Gene |
| keywords[14].id | https://openalex.org/keywords/pectinase |
| keywords[14].score | 0.13634911179542542 |
| keywords[14].display_name | Pectinase |
| language | en |
| locations[0].id | doi:10.3389/fbioe.2023.1251342 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S2595131146 |
| locations[0].source.issn | 2296-4185 |
| locations[0].source.type | journal |
| locations[0].source.is_oa | True |
| locations[0].source.issn_l | 2296-4185 |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | True |
| locations[0].source.display_name | Frontiers in Bioengineering and Biotechnology |
| locations[0].source.host_organization | https://openalex.org/P4310320527 |
| locations[0].source.host_organization_name | Frontiers Media |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310320527 |
| locations[0].license | cc-by |
| locations[0].pdf_url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1251342/pdf |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | https://openalex.org/licenses/cc-by |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | Frontiers in Bioengineering and Biotechnology |
| locations[0].landing_page_url | https://doi.org/10.3389/fbioe.2023.1251342 |
| locations[1].id | pmid:37720319 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | Frontiers in bioengineering and biotechnology |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/37720319 |
| locations[2].id | pmh:oai:pubmedcentral.nih.gov:10502208 |
| locations[2].is_oa | True |
| locations[2].source.id | https://openalex.org/S2764455111 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | PubMed Central |
| locations[2].source.host_organization | https://openalex.org/I1299303238 |
| locations[2].source.host_organization_name | National Institutes of Health |
| locations[2].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[2].license | cc-by |
| locations[2].pdf_url | https://pmc.ncbi.nlm.nih.gov/articles/PMC10502208/pdf/fbioe-11-1251342.pdf |
| locations[2].version | submittedVersion |
| locations[2].raw_type | Text |
| locations[2].license_id | https://openalex.org/licenses/cc-by |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | Front Bioeng Biotechnol |
| locations[2].landing_page_url | https://www.ncbi.nlm.nih.gov/pmc/articles/10502208 |
| locations[3].id | pmh:oai:doaj.org/article:0d185a2900aa4eea927df17f7417b738 |
| locations[3].is_oa | False |
| locations[3].source.id | https://openalex.org/S4306401280 |
| locations[3].source.issn | |
| locations[3].source.type | repository |
| locations[3].source.is_oa | False |
| locations[3].source.issn_l | |
| locations[3].source.is_core | False |
| locations[3].source.is_in_doaj | False |
| locations[3].source.display_name | DOAJ (DOAJ: Directory of Open Access Journals) |
| locations[3].source.host_organization | |
| locations[3].source.host_organization_name | |
| locations[3].source.host_organization_lineage | |
| locations[3].license | |
| locations[3].pdf_url | |
| locations[3].version | submittedVersion |
| locations[3].raw_type | article |
| locations[3].license_id | |
| locations[3].is_accepted | False |
| locations[3].is_published | False |
| locations[3].raw_source_name | Frontiers in Bioengineering and Biotechnology, Vol 11 (2023) |
| locations[3].landing_page_url | https://doaj.org/article/0d185a2900aa4eea927df17f7417b738 |
| indexed_in | crossref, doaj, pubmed |
| authorships[0].author.id | https://openalex.org/A5076774917 |
| authorships[0].author.orcid | https://orcid.org/0000-0001-8776-3833 |
| authorships[0].author.display_name | Ke Fang |
| authorships[0].countries | CN |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I152269853 |
| authorships[0].affiliations[0].raw_affiliation_string | State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[0].affiliations[1].institution_ids | https://openalex.org/I152269853 |
| authorships[0].affiliations[1].raw_affiliation_string | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China |
| authorships[0].institutions[0].id | https://openalex.org/I152269853 |
| authorships[0].institutions[0].ror | https://ror.org/04hyzq608 |
| authorships[0].institutions[0].type | education |
| authorships[0].institutions[0].lineage | https://openalex.org/I152269853 |
| authorships[0].institutions[0].country_code | CN |
| authorships[0].institutions[0].display_name | Qilu University of Technology |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Ke Fang |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China, State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[1].author.id | https://openalex.org/A5066216634 |
| authorships[1].author.orcid | https://orcid.org/0000-0003-0433-0720 |
| authorships[1].author.display_name | Jun Ma |
| authorships[1].countries | CN |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I152269853 |
| authorships[1].affiliations[0].raw_affiliation_string | State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[1].affiliations[1].institution_ids | https://openalex.org/I152269853 |
| authorships[1].affiliations[1].raw_affiliation_string | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China |
| authorships[1].institutions[0].id | https://openalex.org/I152269853 |
| authorships[1].institutions[0].ror | https://ror.org/04hyzq608 |
| authorships[1].institutions[0].type | education |
| authorships[1].institutions[0].lineage | https://openalex.org/I152269853 |
| authorships[1].institutions[0].country_code | CN |
| authorships[1].institutions[0].display_name | Qilu University of Technology |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Jun Ma |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China, State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[2].author.id | https://openalex.org/A5115588883 |
| authorships[2].author.orcid | https://orcid.org/0009-0004-9019-4810 |
| authorships[2].author.display_name | Xinyu Wang |
| authorships[2].countries | CN |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I152269853 |
| authorships[2].affiliations[0].raw_affiliation_string | State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[2].affiliations[1].institution_ids | https://openalex.org/I152269853 |
| authorships[2].affiliations[1].raw_affiliation_string | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China |
| authorships[2].institutions[0].id | https://openalex.org/I152269853 |
| authorships[2].institutions[0].ror | https://ror.org/04hyzq608 |
| authorships[2].institutions[0].type | education |
| authorships[2].institutions[0].lineage | https://openalex.org/I152269853 |
| authorships[2].institutions[0].country_code | CN |
| authorships[2].institutions[0].display_name | Qilu University of Technology |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Xinyu Wang |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China, State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[3].author.id | https://openalex.org/A5101288110 |
| authorships[3].author.orcid | |
| authorships[3].author.display_name | Ziting Xu |
| authorships[3].countries | CN |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I152269853 |
| authorships[3].affiliations[0].raw_affiliation_string | State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[3].affiliations[1].institution_ids | https://openalex.org/I152269853 |
| authorships[3].affiliations[1].raw_affiliation_string | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China |
| authorships[3].institutions[0].id | https://openalex.org/I152269853 |
| authorships[3].institutions[0].ror | https://ror.org/04hyzq608 |
| authorships[3].institutions[0].type | education |
| authorships[3].institutions[0].lineage | https://openalex.org/I152269853 |
| authorships[3].institutions[0].country_code | CN |
| authorships[3].institutions[0].display_name | Qilu University of Technology |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Ziting Xu |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China, State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[4].author.id | https://openalex.org/A5100357829 |
| authorships[4].author.orcid | https://orcid.org/0000-0002-0439-2481 |
| authorships[4].author.display_name | Ziyang Zhang |
| authorships[4].countries | CN |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I152269853 |
| authorships[4].affiliations[0].raw_affiliation_string | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China |
| authorships[4].affiliations[1].institution_ids | https://openalex.org/I152269853 |
| authorships[4].affiliations[1].raw_affiliation_string | State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[4].institutions[0].id | https://openalex.org/I152269853 |
| authorships[4].institutions[0].ror | https://ror.org/04hyzq608 |
| authorships[4].institutions[0].type | education |
| authorships[4].institutions[0].lineage | https://openalex.org/I152269853 |
| authorships[4].institutions[0].country_code | CN |
| authorships[4].institutions[0].display_name | Qilu University of Technology |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Ziyang Zhang |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China, State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[5].author.id | https://openalex.org/A5023773475 |
| authorships[5].author.orcid | https://orcid.org/0000-0002-2134-4641 |
| authorships[5].author.display_name | Piwu Li |
| authorships[5].countries | CN |
| authorships[5].affiliations[0].institution_ids | https://openalex.org/I152269853 |
| authorships[5].affiliations[0].raw_affiliation_string | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China |
| authorships[5].affiliations[1].institution_ids | https://openalex.org/I152269853 |
| authorships[5].affiliations[1].raw_affiliation_string | State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[5].institutions[0].id | https://openalex.org/I152269853 |
| authorships[5].institutions[0].ror | https://ror.org/04hyzq608 |
| authorships[5].institutions[0].type | education |
| authorships[5].institutions[0].lineage | https://openalex.org/I152269853 |
| authorships[5].institutions[0].country_code | CN |
| authorships[5].institutions[0].display_name | Qilu University of Technology |
| authorships[5].author_position | middle |
| authorships[5].raw_author_name | Piwu Li |
| authorships[5].is_corresponding | False |
| authorships[5].raw_affiliation_strings | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China, State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[6].author.id | https://openalex.org/A5101707179 |
| authorships[6].author.orcid | https://orcid.org/0009-0008-9902-0875 |
| authorships[6].author.display_name | Ruiming Wang |
| authorships[6].countries | CN |
| authorships[6].affiliations[0].institution_ids | https://openalex.org/I152269853 |
| authorships[6].affiliations[0].raw_affiliation_string | State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[6].affiliations[1].institution_ids | https://openalex.org/I152269853 |
| authorships[6].affiliations[1].raw_affiliation_string | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China |
| authorships[6].institutions[0].id | https://openalex.org/I152269853 |
| authorships[6].institutions[0].ror | https://ror.org/04hyzq608 |
| authorships[6].institutions[0].type | education |
| authorships[6].institutions[0].lineage | https://openalex.org/I152269853 |
| authorships[6].institutions[0].country_code | CN |
| authorships[6].institutions[0].display_name | Qilu University of Technology |
| authorships[6].author_position | middle |
| authorships[6].raw_author_name | Ruiming Wang |
| authorships[6].is_corresponding | False |
| authorships[6].raw_affiliation_strings | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China, State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[7].author.id | https://openalex.org/A5100670154 |
| authorships[7].author.orcid | https://orcid.org/0000-0001-9862-2366 |
| authorships[7].author.display_name | Junqing Wang |
| authorships[7].countries | CN |
| authorships[7].affiliations[0].institution_ids | https://openalex.org/I152269853 |
| authorships[7].affiliations[0].raw_affiliation_string | State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[7].affiliations[1].institution_ids | https://openalex.org/I152269853 |
| authorships[7].affiliations[1].raw_affiliation_string | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China |
| authorships[7].institutions[0].id | https://openalex.org/I152269853 |
| authorships[7].institutions[0].ror | https://ror.org/04hyzq608 |
| authorships[7].institutions[0].type | education |
| authorships[7].institutions[0].lineage | https://openalex.org/I152269853 |
| authorships[7].institutions[0].country_code | CN |
| authorships[7].institutions[0].display_name | Qilu University of Technology |
| authorships[7].author_position | middle |
| authorships[7].raw_author_name | Junqing Wang |
| authorships[7].is_corresponding | True |
| authorships[7].raw_affiliation_strings | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China, State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[8].author.id | https://openalex.org/A5111032801 |
| authorships[8].author.orcid | |
| authorships[8].author.display_name | Chuying Sun |
| authorships[8].countries | CN |
| authorships[8].affiliations[0].institution_ids | https://openalex.org/I152269853 |
| authorships[8].affiliations[0].raw_affiliation_string | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China |
| authorships[8].affiliations[1].institution_ids | https://openalex.org/I152269853 |
| authorships[8].affiliations[1].raw_affiliation_string | State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[8].institutions[0].id | https://openalex.org/I152269853 |
| authorships[8].institutions[0].ror | https://ror.org/04hyzq608 |
| authorships[8].institutions[0].type | education |
| authorships[8].institutions[0].lineage | https://openalex.org/I152269853 |
| authorships[8].institutions[0].country_code | CN |
| authorships[8].institutions[0].display_name | Qilu University of Technology |
| authorships[8].author_position | middle |
| authorships[8].raw_author_name | Chuying Sun |
| authorships[8].is_corresponding | False |
| authorships[8].raw_affiliation_strings | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China, State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[9].author.id | https://openalex.org/A5021690119 |
| authorships[9].author.orcid | |
| authorships[9].author.display_name | Ziyang Dong |
| authorships[9].countries | CN |
| authorships[9].affiliations[0].institution_ids | https://openalex.org/I152269853 |
| authorships[9].affiliations[0].raw_affiliation_string | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China |
| authorships[9].affiliations[1].institution_ids | https://openalex.org/I152269853 |
| authorships[9].affiliations[1].raw_affiliation_string | State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| authorships[9].institutions[0].id | https://openalex.org/I152269853 |
| authorships[9].institutions[0].ror | https://ror.org/04hyzq608 |
| authorships[9].institutions[0].type | education |
| authorships[9].institutions[0].lineage | https://openalex.org/I152269853 |
| authorships[9].institutions[0].country_code | CN |
| authorships[9].institutions[0].display_name | Qilu University of Technology |
| authorships[9].author_position | last |
| authorships[9].raw_author_name | Ziyang Dong |
| authorships[9].is_corresponding | False |
| authorships[9].raw_affiliation_strings | School of Bioengineering, Qilu University of Technology, Jinan, Shandong, China, State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China |
| has_content.pdf | True |
| has_content.grobid_xml | True |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1251342/pdf |
| open_access.oa_status | gold |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Flow-cytometric cell sorting coupled with UV mutagenesis for improving pectin lyase expression |
| has_fulltext | True |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T10869 |
| primary_topic.field.id | https://openalex.org/fields/11 |
| primary_topic.field.display_name | Agricultural and Biological Sciences |
| primary_topic.score | 0.9965000152587891 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1110 |
| primary_topic.subfield.display_name | Plant Science |
| primary_topic.display_name | Polysaccharides and Plant Cell Walls |
| related_works | https://openalex.org/W1967452069, https://openalex.org/W2021429516, https://openalex.org/W2039660951, https://openalex.org/W2085718839, https://openalex.org/W2020925881, https://openalex.org/W1964950631, https://openalex.org/W2003673012, https://openalex.org/W1980133119, https://openalex.org/W1974810365, https://openalex.org/W2787601911 |
| cited_by_count | 5 |
| counts_by_year[0].year | 2025 |
| counts_by_year[0].cited_by_count | 4 |
| counts_by_year[1].year | 2024 |
| counts_by_year[1].cited_by_count | 1 |
| locations_count | 4 |
| best_oa_location.id | doi:10.3389/fbioe.2023.1251342 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S2595131146 |
| best_oa_location.source.issn | 2296-4185 |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | True |
| best_oa_location.source.issn_l | 2296-4185 |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | True |
| best_oa_location.source.display_name | Frontiers in Bioengineering and Biotechnology |
| best_oa_location.source.host_organization | https://openalex.org/P4310320527 |
| best_oa_location.source.host_organization_name | Frontiers Media |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310320527 |
| best_oa_location.license | cc-by |
| best_oa_location.pdf_url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1251342/pdf |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | Frontiers in Bioengineering and Biotechnology |
| best_oa_location.landing_page_url | https://doi.org/10.3389/fbioe.2023.1251342 |
| primary_location.id | doi:10.3389/fbioe.2023.1251342 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S2595131146 |
| primary_location.source.issn | 2296-4185 |
| primary_location.source.type | journal |
| primary_location.source.is_oa | True |
| primary_location.source.issn_l | 2296-4185 |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | True |
| primary_location.source.display_name | Frontiers in Bioengineering and Biotechnology |
| primary_location.source.host_organization | https://openalex.org/P4310320527 |
| primary_location.source.host_organization_name | Frontiers Media |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310320527 |
| primary_location.license | cc-by |
| primary_location.pdf_url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1251342/pdf |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | https://openalex.org/licenses/cc-by |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | Frontiers in Bioengineering and Biotechnology |
| primary_location.landing_page_url | https://doi.org/10.3389/fbioe.2023.1251342 |
| publication_date | 2023-08-31 |
| publication_year | 2023 |
| referenced_works | https://openalex.org/W3012364343, https://openalex.org/W2056710301, https://openalex.org/W3208445926, https://openalex.org/W1978443163, https://openalex.org/W2577225940, https://openalex.org/W2306996931, https://openalex.org/W2081164013, https://openalex.org/W2057066155, https://openalex.org/W4293246144, https://openalex.org/W2767907586, https://openalex.org/W1257431287, https://openalex.org/W3007126735, https://openalex.org/W998174307, https://openalex.org/W2129361946, https://openalex.org/W4311672565, https://openalex.org/W2939379351, https://openalex.org/W2747929863, https://openalex.org/W4303982423, https://openalex.org/W2136708509, https://openalex.org/W4303940479, https://openalex.org/W2764096616, https://openalex.org/W2992449748, https://openalex.org/W2005695868, https://openalex.org/W2019081063, https://openalex.org/W4283798275, https://openalex.org/W2037729655, https://openalex.org/W1939692840, https://openalex.org/W2980993562, https://openalex.org/W2969556953, https://openalex.org/W3217167949, https://openalex.org/W3120966921, https://openalex.org/W2966201978, https://openalex.org/W4361264538, https://openalex.org/W2053359824, https://openalex.org/W4281761874, https://openalex.org/W3137614919, https://openalex.org/W2049952619, https://openalex.org/W2622444636, https://openalex.org/W2157774942, https://openalex.org/W3008069394, https://openalex.org/W2959838452, https://openalex.org/W3193754864, https://openalex.org/W2586861723 |
| referenced_works_count | 43 |
| abstract_inverted_index.- | 144 |
| abstract_inverted_index.E | 254 |
| abstract_inverted_index.a | 9, 59, 134, 142 |
| abstract_inverted_index.p | 233 |
| abstract_inverted_index.E) | 256 |
| abstract_inverted_index.E. | 216, 229, 262, 274 |
| abstract_inverted_index.It | 17 |
| abstract_inverted_index.UV | 169 |
| abstract_inverted_index.We | 95, 123 |
| abstract_inverted_index.an | 5, 158, 291 |
| abstract_inverted_index.as | 27, 58 |
| abstract_inverted_index.at | 68, 244, 302 |
| abstract_inverted_index.be | 281 |
| abstract_inverted_index.by | 101 |
| abstract_inverted_index.h. | 246 |
| abstract_inverted_index.in | 14, 22, 86, 215, 226, 271 |
| abstract_inverted_index.is | 4 |
| abstract_inverted_index.of | 12, 37, 261, 297 |
| abstract_inverted_index.pH | 69 |
| abstract_inverted_index.to | 84, 90, 97, 109, 224, 268 |
| abstract_inverted_index.144 | 245 |
| abstract_inverted_index.240 | 242 |
| abstract_inverted_index.Our | 288 |
| abstract_inverted_index.The | 148, 188 |
| abstract_inverted_index.and | 31, 35, 39, 49, 51, 71, 119, 129, 138, 161, 176, 195, 210, 236, 257 |
| abstract_inverted_index.are | 81 |
| abstract_inverted_index.due | 89 |
| abstract_inverted_index.for | 294 |
| abstract_inverted_index.has | 18 |
| abstract_inverted_index.the | 33, 74, 125, 179, 196, 205, 227, 237, 269, 272, 295 |
| abstract_inverted_index.via | 163 |
| abstract_inverted_index.was | 55, 154, 186, 193, 201, 219, 240 |
| abstract_inverted_index.230, | 241 |
| abstract_inverted_index.BL21 | 276 |
| abstract_inverted_index.U/mL | 243 |
| abstract_inverted_index.been | 19 |
| abstract_inverted_index.cell | 165 |
| abstract_inverted_index.coli | 184, 217, 230, 263, 275 |
| abstract_inverted_index.egfp | 132, 153, 192 |
| abstract_inverted_index.high | 65, 303 |
| abstract_inverted_index.hope | 96 |
| abstract_inverted_index.host | 160 |
| abstract_inverted_index.into | 141, 157, 204 |
| abstract_inverted_index.many | 23 |
| abstract_inverted_index.pulp | 50 |
| abstract_inverted_index.such | 26 |
| abstract_inverted_index.that | 225, 250 |
| abstract_inverted_index.them | 140 |
| abstract_inverted_index.then | 155, 202 |
| abstract_inverted_index.used | 21 |
| abstract_inverted_index.were | 265 |
| abstract_inverted_index.wide | 10 |
| abstract_inverted_index.with | 8, 64, 106, 115, 168, 283 |
| abstract_inverted_index.work | 289 |
| abstract_inverted_index.(DE3) | 277 |
| abstract_inverted_index.After | 208 |
| abstract_inverted_index.apply | 85 |
| abstract_inverted_index.cost. | 121 |
| abstract_inverted_index.could | 280 |
| abstract_inverted_index.fruit | 28 |
| abstract_inverted_index.fused | 124 |
| abstract_inverted_index.genes | 126, 251 |
| abstract_inverted_index.juice | 29 |
| abstract_inverted_index.linen | 40 |
| abstract_inverted_index.lower | 120 |
| abstract_inverted_index.lyase | 3, 63, 78, 114, 213, 301 |
| abstract_inverted_index.named | 182 |
| abstract_inverted_index.paper | 52 |
| abstract_inverted_index.plant | 43 |
| abstract_inverted_index.range | 11 |
| abstract_inverted_index.solve | 98 |
| abstract_inverted_index.still | 82 |
| abstract_inverted_index.their | 91 |
| abstract_inverted_index.these | 99 |
| abstract_inverted_index.using | 133 |
| abstract_inverted_index.which | 279 |
| abstract_inverted_index.(ApaH) | 260 |
| abstract_inverted_index.(RNase | 255 |
| abstract_inverted_index.70°C. | 72 |
| abstract_inverted_index.Genome | 247 |
| abstract_inverted_index.cotton | 38 |
| abstract_inverted_index.dyeing | 34 |
| abstract_inverted_index.enzyme | 7, 285 |
| abstract_inverted_index.fields | 25 |
| abstract_inverted_index.higher | 116 |
| abstract_inverted_index.linker | 136 |
| abstract_inverted_index.method | 293 |
| abstract_inverted_index.modern | 103 |
| abstract_inverted_index.mutant | 206 |
| abstract_inverted_index.pKD46. | 147 |
| abstract_inverted_index.pectin | 2, 62, 77, 113, 212, 300 |
| abstract_inverted_index.screen | 111 |
| abstract_inverted_index.widely | 20 |
| abstract_inverted_index.0.001), | 235 |
| abstract_inverted_index.11.0°C | 70 |
| abstract_inverted_index.coupled | 167 |
| abstract_inverted_index.fibers, | 44 |
| abstract_inverted_index.highest | 238 |
| abstract_inverted_index.levels. | 304 |
| abstract_inverted_index.ligated | 139 |
| abstract_inverted_index.limited | 92 |
| abstract_inverted_index.mutated | 266 |
| abstract_inverted_index.peptide | 137 |
| abstract_inverted_index.plasmid | 151, 190, 199 |
| abstract_inverted_index.primary | 174 |
| abstract_inverted_index.protein | 131 |
| abstract_inverted_index.rapidly | 110 |
| abstract_inverted_index.sorting | 166 |
| abstract_inverted_index.strain, | 181, 232, 278 |
| abstract_inverted_index.strains | 80, 298 |
| abstract_inverted_index.&lt; | 234 |
| abstract_inverted_index.Alkaline | 1 |
| abstract_inverted_index.BL21/1G3 | 218, 264 |
| abstract_inverted_index.However, | 73 |
| abstract_inverted_index.Methods: | 122 |
| abstract_inverted_index.Results: | 171 |
| abstract_inverted_index.activity | 67, 118, 214, 239 |
| abstract_inverted_index.alkaline | 61, 112 |
| abstract_inverted_index.breeding | 104 |
| abstract_inverted_index.compared | 267 |
| abstract_inverted_index.encoding | 127, 252 |
| abstract_inverted_index.flexible | 135 |
| abstract_inverted_index.modified | 60 |
| abstract_inverted_index.original | 197, 273 |
| abstract_inverted_index.parental | 228 |
| abstract_inverted_index.plasmid, | 146 |
| abstract_inverted_index.problems | 100 |
| abstract_inverted_index.provides | 290 |
| abstract_inverted_index.relative | 223 |
| abstract_inverted_index.revealed | 249 |
| abstract_inverted_index.screened | 162 |
| abstract_inverted_index.sequence | 270 |
| abstract_inverted_index.specific | 66 |
| abstract_inverted_index.strains. | 207 |
| abstract_inverted_index.BL21/1G3, | 185 |
| abstract_inverted_index.Following | 172 |
| abstract_inverted_index.PGLA-rep4 | 54, 128 |
| abstract_inverted_index.activity. | 94 |
| abstract_inverted_index.combining | 102 |
| abstract_inverted_index.degumming | 42 |
| abstract_inverted_index.difficult | 83 |
| abstract_inverted_index.effective | 292 |
| abstract_inverted_index.enzymatic | 93, 117 |
| abstract_inverted_index.equipment | 108 |
| abstract_inverted_index.generated | 57 |
| abstract_inverted_index.important | 6, 24 |
| abstract_inverted_index.increased | 221, 284 |
| abstract_inverted_index.induction | 209 |
| abstract_inverted_index.obtained. | 187 |
| abstract_inverted_index.screening | 150, 189 |
| abstract_inverted_index.secondary | 177 |
| abstract_inverted_index.sensitive | 145 |
| abstract_inverted_index.textiles, | 41 |
| abstract_inverted_index.(1.37-fold | 222 |
| abstract_inverted_index.associated | 282 |
| abstract_inverted_index.expressing | 299 |
| abstract_inverted_index.expression | 79, 159, 198 |
| abstract_inverted_index.industrial | 15, 46, 87 |
| abstract_inverted_index.previously | 56 |
| abstract_inverted_index.processing | 30, 36 |
| abstract_inverted_index.production | 88 |
| abstract_inverted_index.screening, | 175, 178 |
| abstract_inverted_index.sequencing | 248 |
| abstract_inverted_index.techniques | 105 |
| abstract_inverted_index.treatment, | 48 |
| abstract_inverted_index.wastewater | 47 |
| abstract_inverted_index.Discussion: | 287 |
| abstract_inverted_index.Escherichia | 183 |
| abstract_inverted_index.constructed | 149 |
| abstract_inverted_index.diadenosine | 258 |
| abstract_inverted_index.eliminated, | 194 |
| abstract_inverted_index.expression. | 286 |
| abstract_inverted_index.extraction, | 32 |
| abstract_inverted_index.fluorescent | 130 |
| abstract_inverted_index.production, | 16 |
| abstract_inverted_index.production. | 53 |
| abstract_inverted_index.temperature | 143 |
| abstract_inverted_index.transformed | 156 |
| abstract_inverted_index.applications | 13 |
| abstract_inverted_index.construction | 296 |
| abstract_inverted_index.mutagenesis, | 173 |
| abstract_inverted_index.mutagenesis. | 170 |
| abstract_inverted_index.ribonuclease | 253 |
| abstract_inverted_index.Introduction: | 0 |
| abstract_inverted_index.environmental | 45 |
| abstract_inverted_index.fermentation, | 211 |
| abstract_inverted_index.high-activity | 76 |
| abstract_inverted_index.retransformed | 203 |
| abstract_inverted_index.significantly | 220 |
| abstract_inverted_index.BL21/PGLA-rep4 | 231 |
| abstract_inverted_index.flow-cytometric | 164 |
| abstract_inverted_index.high-expression | 180 |
| abstract_inverted_index.high-throughput | 107 |
| abstract_inverted_index.pre-constructed | 75 |
| abstract_inverted_index.pET28a-PGLA-rep4 | 200 |
| abstract_inverted_index.pKD46-PGLA-rep4- | 152, 191 |
| abstract_inverted_index.tetraphosphatase | 259 |
| cited_by_percentile_year.max | 98 |
| cited_by_percentile_year.min | 90 |
| corresponding_author_ids | https://openalex.org/A5100670154 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 10 |
| corresponding_institution_ids | https://openalex.org/I152269853 |
| sustainable_development_goals[0].id | https://metadata.un.org/sdg/6 |
| sustainable_development_goals[0].score | 0.8600000143051147 |
| sustainable_development_goals[0].display_name | Clean water and sanitation |
| citation_normalized_percentile.value | 0.89242367 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |