Fluorescence Bar-Coding and Flowmetry Based on Dark State Transitions in Fluorescence Emitters Article Swipe
Elin Sandberg
,
Barış Demirbay
,
Abhilash Kulkarni
,
Haichun Liu
,
Joachim Piguet
,
Jerker Widengren
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1021/acs.jpcb.3c06905
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1021/acs.jpcb.3c06905
Reversible dark state transitions in fluorophores represent a limiting factor in fluorescence-based ultrasensitive spectroscopy, are a necessary basis for fluorescence-based super-resolution imaging, but may also offer additional, largely orthogonal fluorescence-based readout parameters. In this work, we analyzed the blinking kinetics of Cyanine5 (Cy5) as a bar-coding feature distinguishing Cy5 from rhodamine fluorophores having largely overlapping emission spectra. First, fluorescence correlation spectroscopy (FCS) solution measurements on mixtures of free fluorophores and fluorophore-labeled small unilamellar vesicles (SUVs) showed that Cy5 could be readily distinguished from the rhodamines by its reversible, largely excitation-driven trans-cis isomerization. This was next confirmed by transient state (TRAST) spectroscopy measurements, determining the fluorophore dark state kinetics in a more robust manner, from how the time-averaged fluorescence intensity varies upon modulation of the applied excitation light. TRAST was then combined with wide-field imaging of live cells, whereby Cy5 and rhodamine fluorophores could be distinguished on a whole cell level as well as in spatially resolved, multiplexed images of the cells. Finally, we established a microfluidic TRAST concept and showed how different mixtures of free Cy5 and rhodamine fluorophores and corresponding fluorophore-labeled SUVs could be distinguished on-the-fly when passing through a microfluidic channel. In contrast to FCS, TRAST does not rely on single-molecule detection conditions or a high time resolution and is thus broadly applicable to different biological samples. Therefore, we expect that the bar-coding concept presented in this work can offer an additional useful strategy for fluorescence-based multiplexing that can be implemented on a broad range of both stationary and moving samples.
Related Topics
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1021/acs.jpcb.3c06905
- OA Status
- hybrid
- Cited By
- 6
- References
- 42
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4390048627
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W4390048627Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1021/acs.jpcb.3c06905Digital Object Identifier
- Title
-
Fluorescence Bar-Coding and Flowmetry Based on Dark State Transitions in Fluorescence EmittersWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2023Year of publication
- Publication date
-
2023-12-21Full publication date if available
- Authors
-
Elin Sandberg, Barış Demirbay, Abhilash Kulkarni, Haichun Liu, Joachim Piguet, Jerker WidengrenList of authors in order
- Landing page
-
https://doi.org/10.1021/acs.jpcb.3c06905Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
hybridOpen access status per OpenAlex
- OA URL
-
https://doi.org/10.1021/acs.jpcb.3c06905Direct OA link when available
- Concepts
-
Fluorescence, Bar (unit), Materials science, Physics, Optics, MeteorologyTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
6Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 5, 2024: 1Per-year citation counts (last 5 years)
- References (count)
-
42Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W4390048627 |
|---|---|
| doi | https://doi.org/10.1021/acs.jpcb.3c06905 |
| ids.doi | https://doi.org/10.1021/acs.jpcb.3c06905 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/38127267 |
| ids.openalex | https://openalex.org/W4390048627 |
| fwci | 1.8471087 |
| mesh[0].qualifier_ui | Q000379 |
| mesh[0].descriptor_ui | D013050 |
| mesh[0].is_major_topic | False |
| mesh[0].qualifier_name | methods |
| mesh[0].descriptor_name | Spectrometry, Fluorescence |
| mesh[1].qualifier_ui | Q000737 |
| mesh[1].descriptor_ui | D002232 |
| mesh[1].is_major_topic | False |
| mesh[1].qualifier_name | chemistry |
| mesh[1].descriptor_name | Carbocyanines |
| mesh[2].qualifier_ui | |
| mesh[2].descriptor_ui | D012235 |
| mesh[2].is_major_topic | False |
| mesh[2].qualifier_name | |
| mesh[2].descriptor_name | Rhodamines |
| mesh[3].qualifier_ui | Q000737 |
| mesh[3].descriptor_ui | D005456 |
| mesh[3].is_major_topic | True |
| mesh[3].qualifier_name | chemistry |
| mesh[3].descriptor_name | Fluorescent Dyes |
| mesh[4].qualifier_ui | |
| mesh[4].descriptor_ui | D044085 |
| mesh[4].is_major_topic | True |
| mesh[4].qualifier_name | |
| mesh[4].descriptor_name | Microfluidics |
| mesh[5].qualifier_ui | Q000379 |
| mesh[5].descriptor_ui | D013050 |
| mesh[5].is_major_topic | False |
| mesh[5].qualifier_name | methods |
| mesh[5].descriptor_name | Spectrometry, Fluorescence |
| mesh[6].qualifier_ui | Q000737 |
| mesh[6].descriptor_ui | D002232 |
| mesh[6].is_major_topic | False |
| mesh[6].qualifier_name | chemistry |
| mesh[6].descriptor_name | Carbocyanines |
| mesh[7].qualifier_ui | |
| mesh[7].descriptor_ui | D012235 |
| mesh[7].is_major_topic | False |
| mesh[7].qualifier_name | |
| mesh[7].descriptor_name | Rhodamines |
| mesh[8].qualifier_ui | Q000737 |
| mesh[8].descriptor_ui | D005456 |
| mesh[8].is_major_topic | True |
| mesh[8].qualifier_name | chemistry |
| mesh[8].descriptor_name | Fluorescent Dyes |
| mesh[9].qualifier_ui | |
| mesh[9].descriptor_ui | D044085 |
| mesh[9].is_major_topic | True |
| mesh[9].qualifier_name | |
| mesh[9].descriptor_name | Microfluidics |
| mesh[10].qualifier_ui | Q000379 |
| mesh[10].descriptor_ui | D013050 |
| mesh[10].is_major_topic | False |
| mesh[10].qualifier_name | methods |
| mesh[10].descriptor_name | Spectrometry, Fluorescence |
| mesh[11].qualifier_ui | Q000737 |
| mesh[11].descriptor_ui | D002232 |
| mesh[11].is_major_topic | False |
| mesh[11].qualifier_name | chemistry |
| mesh[11].descriptor_name | Carbocyanines |
| mesh[12].qualifier_ui | |
| mesh[12].descriptor_ui | D012235 |
| mesh[12].is_major_topic | False |
| mesh[12].qualifier_name | |
| mesh[12].descriptor_name | Rhodamines |
| mesh[13].qualifier_ui | Q000737 |
| mesh[13].descriptor_ui | D005456 |
| mesh[13].is_major_topic | True |
| mesh[13].qualifier_name | chemistry |
| mesh[13].descriptor_name | Fluorescent Dyes |
| mesh[14].qualifier_ui | |
| mesh[14].descriptor_ui | D044085 |
| mesh[14].is_major_topic | True |
| mesh[14].qualifier_name | |
| mesh[14].descriptor_name | Microfluidics |
| mesh[15].qualifier_ui | Q000379 |
| mesh[15].descriptor_ui | D013050 |
| mesh[15].is_major_topic | False |
| mesh[15].qualifier_name | methods |
| mesh[15].descriptor_name | Spectrometry, Fluorescence |
| mesh[16].qualifier_ui | Q000737 |
| mesh[16].descriptor_ui | D002232 |
| mesh[16].is_major_topic | False |
| mesh[16].qualifier_name | chemistry |
| mesh[16].descriptor_name | Carbocyanines |
| mesh[17].qualifier_ui | |
| mesh[17].descriptor_ui | D012235 |
| mesh[17].is_major_topic | False |
| mesh[17].qualifier_name | |
| mesh[17].descriptor_name | Rhodamines |
| mesh[18].qualifier_ui | Q000737 |
| mesh[18].descriptor_ui | D005456 |
| mesh[18].is_major_topic | True |
| mesh[18].qualifier_name | chemistry |
| mesh[18].descriptor_name | Fluorescent Dyes |
| mesh[19].qualifier_ui | |
| mesh[19].descriptor_ui | D044085 |
| mesh[19].is_major_topic | True |
| mesh[19].qualifier_name | |
| mesh[19].descriptor_name | Microfluidics |
| mesh[20].qualifier_ui | Q000379 |
| mesh[20].descriptor_ui | D013050 |
| mesh[20].is_major_topic | False |
| mesh[20].qualifier_name | methods |
| mesh[20].descriptor_name | Spectrometry, Fluorescence |
| mesh[21].qualifier_ui | Q000737 |
| mesh[21].descriptor_ui | D002232 |
| mesh[21].is_major_topic | False |
| mesh[21].qualifier_name | chemistry |
| mesh[21].descriptor_name | Carbocyanines |
| mesh[22].qualifier_ui | |
| mesh[22].descriptor_ui | D012235 |
| mesh[22].is_major_topic | False |
| mesh[22].qualifier_name | |
| mesh[22].descriptor_name | Rhodamines |
| mesh[23].qualifier_ui | Q000737 |
| mesh[23].descriptor_ui | D005456 |
| mesh[23].is_major_topic | True |
| mesh[23].qualifier_name | chemistry |
| mesh[23].descriptor_name | Fluorescent Dyes |
| mesh[24].qualifier_ui | |
| mesh[24].descriptor_ui | D044085 |
| mesh[24].is_major_topic | True |
| mesh[24].qualifier_name | |
| mesh[24].descriptor_name | Microfluidics |
| type | article |
| title | Fluorescence Bar-Coding and Flowmetry Based on Dark State Transitions in Fluorescence Emitters |
| awards[0].id | https://openalex.org/G2475504986 |
| awards[0].funder_id | https://openalex.org/F4320322581 |
| awards[0].display_name | |
| awards[0].funder_award_id | VR 2021-04556 |
| awards[0].funder_display_name | Vetenskapsrådet |
| awards[1].id | https://openalex.org/G6350851456 |
| awards[1].funder_id | https://openalex.org/F4320320940 |
| awards[1].display_name | |
| awards[1].funder_award_id | RMX18-0041 |
| awards[1].funder_display_name | Stiftelsen för Strategisk Forskning |
| biblio.issue | 1 |
| biblio.volume | 128 |
| biblio.last_page | 136 |
| biblio.first_page | 125 |
| topics[0].id | https://openalex.org/T10540 |
| topics[0].field.id | https://openalex.org/fields/13 |
| topics[0].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[0].score | 0.9937000274658203 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1304 |
| topics[0].subfield.display_name | Biophysics |
| topics[0].display_name | Advanced Fluorescence Microscopy Techniques |
| topics[1].id | https://openalex.org/T10558 |
| topics[1].field.id | https://openalex.org/fields/22 |
| topics[1].field.display_name | Engineering |
| topics[1].score | 0.9812999963760376 |
| topics[1].domain.id | https://openalex.org/domains/3 |
| topics[1].domain.display_name | Physical Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/2208 |
| topics[1].subfield.display_name | Electrical and Electronic Engineering |
| topics[1].display_name | Advancements in Semiconductor Devices and Circuit Design |
| topics[2].id | https://openalex.org/T11992 |
| topics[2].field.id | https://openalex.org/fields/22 |
| topics[2].field.display_name | Engineering |
| topics[2].score | 0.98089998960495 |
| topics[2].domain.id | https://openalex.org/domains/3 |
| topics[2].domain.display_name | Physical Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/2208 |
| topics[2].subfield.display_name | Electrical and Electronic Engineering |
| topics[2].display_name | CCD and CMOS Imaging Sensors |
| funders[0].id | https://openalex.org/F4320320940 |
| funders[0].ror | https://ror.org/044wr7g58 |
| funders[0].display_name | Stiftelsen för Strategisk Forskning |
| funders[1].id | https://openalex.org/F4320322327 |
| funders[1].ror | https://ror.org/004hzzk67 |
| funders[1].display_name | Knut och Alice Wallenbergs Stiftelse |
| funders[2].id | https://openalex.org/F4320322581 |
| funders[2].ror | https://ror.org/03zttf063 |
| funders[2].display_name | Vetenskapsrådet |
| is_xpac | False |
| apc_list | |
| apc_paid | |
| concepts[0].id | https://openalex.org/C91881484 |
| concepts[0].level | 2 |
| concepts[0].score | 0.7805184125900269 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q191807 |
| concepts[0].display_name | Fluorescence |
| concepts[1].id | https://openalex.org/C188721877 |
| concepts[1].level | 2 |
| concepts[1].score | 0.5473537445068359 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q103510 |
| concepts[1].display_name | Bar (unit) |
| concepts[2].id | https://openalex.org/C192562407 |
| concepts[2].level | 0 |
| concepts[2].score | 0.33199965953826904 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q228736 |
| concepts[2].display_name | Materials science |
| concepts[3].id | https://openalex.org/C121332964 |
| concepts[3].level | 0 |
| concepts[3].score | 0.26486778259277344 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q413 |
| concepts[3].display_name | Physics |
| concepts[4].id | https://openalex.org/C120665830 |
| concepts[4].level | 1 |
| concepts[4].score | 0.19294074177742004 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q14620 |
| concepts[4].display_name | Optics |
| concepts[5].id | https://openalex.org/C153294291 |
| concepts[5].level | 1 |
| concepts[5].score | 0.0 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q25261 |
| concepts[5].display_name | Meteorology |
| keywords[0].id | https://openalex.org/keywords/fluorescence |
| keywords[0].score | 0.7805184125900269 |
| keywords[0].display_name | Fluorescence |
| keywords[1].id | https://openalex.org/keywords/bar |
| keywords[1].score | 0.5473537445068359 |
| keywords[1].display_name | Bar (unit) |
| keywords[2].id | https://openalex.org/keywords/materials-science |
| keywords[2].score | 0.33199965953826904 |
| keywords[2].display_name | Materials science |
| keywords[3].id | https://openalex.org/keywords/physics |
| keywords[3].score | 0.26486778259277344 |
| keywords[3].display_name | Physics |
| keywords[4].id | https://openalex.org/keywords/optics |
| keywords[4].score | 0.19294074177742004 |
| keywords[4].display_name | Optics |
| language | en |
| locations[0].id | doi:10.1021/acs.jpcb.3c06905 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S185621672 |
| locations[0].source.issn | 1520-5207, 1520-6106 |
| locations[0].source.type | journal |
| locations[0].source.is_oa | False |
| locations[0].source.issn_l | 1520-5207 |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | False |
| locations[0].source.display_name | The Journal of Physical Chemistry B |
| locations[0].source.host_organization | https://openalex.org/P4310320006 |
| locations[0].source.host_organization_name | American Chemical Society |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310320006 |
| locations[0].source.host_organization_lineage_names | American Chemical Society |
| locations[0].license | cc-by |
| locations[0].pdf_url | |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | https://openalex.org/licenses/cc-by |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | The Journal of Physical Chemistry B |
| locations[0].landing_page_url | https://doi.org/10.1021/acs.jpcb.3c06905 |
| locations[1].id | pmid:38127267 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | The journal of physical chemistry. B |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/38127267 |
| locations[2].id | pmh:oai:pubmedcentral.nih.gov:10788918 |
| locations[2].is_oa | True |
| locations[2].source.id | https://openalex.org/S2764455111 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | PubMed Central |
| locations[2].source.host_organization | https://openalex.org/I1299303238 |
| locations[2].source.host_organization_name | National Institutes of Health |
| locations[2].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[2].license | cc-by |
| locations[2].pdf_url | https://pmc.ncbi.nlm.nih.gov/articles/PMC10788918/pdf/jp3c06905.pdf |
| locations[2].version | submittedVersion |
| locations[2].raw_type | Text |
| locations[2].license_id | https://openalex.org/licenses/cc-by |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | J Phys Chem B |
| locations[2].landing_page_url | https://www.ncbi.nlm.nih.gov/pmc/articles/10788918 |
| indexed_in | crossref, pubmed |
| authorships[0].author.id | https://openalex.org/A5004925265 |
| authorships[0].author.orcid | https://orcid.org/0000-0003-3252-694X |
| authorships[0].author.display_name | Elin Sandberg |
| authorships[0].countries | SE |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I86987016 |
| authorships[0].affiliations[0].raw_affiliation_string | Royal Institute of Technology (KTH), Experimental Biomolecular Physics, Dept. Applied Physics, Albanova University Center, 106 91 Stockholm, Sweden |
| authorships[0].institutions[0].id | https://openalex.org/I86987016 |
| authorships[0].institutions[0].ror | https://ror.org/026vcq606 |
| authorships[0].institutions[0].type | education |
| authorships[0].institutions[0].lineage | https://openalex.org/I86987016 |
| authorships[0].institutions[0].country_code | SE |
| authorships[0].institutions[0].display_name | KTH Royal Institute of Technology |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Elin Sandberg |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | Royal Institute of Technology (KTH), Experimental Biomolecular Physics, Dept. Applied Physics, Albanova University Center, 106 91 Stockholm, Sweden |
| authorships[1].author.id | https://openalex.org/A5001903731 |
| authorships[1].author.orcid | https://orcid.org/0000-0002-5454-7437 |
| authorships[1].author.display_name | Barış Demirbay |
| authorships[1].countries | SE |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I86987016 |
| authorships[1].affiliations[0].raw_affiliation_string | Royal Institute of Technology (KTH), Experimental Biomolecular Physics, Dept. Applied Physics, Albanova University Center, 106 91 Stockholm, Sweden |
| authorships[1].institutions[0].id | https://openalex.org/I86987016 |
| authorships[1].institutions[0].ror | https://ror.org/026vcq606 |
| authorships[1].institutions[0].type | education |
| authorships[1].institutions[0].lineage | https://openalex.org/I86987016 |
| authorships[1].institutions[0].country_code | SE |
| authorships[1].institutions[0].display_name | KTH Royal Institute of Technology |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Baris Demirbay |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Royal Institute of Technology (KTH), Experimental Biomolecular Physics, Dept. Applied Physics, Albanova University Center, 106 91 Stockholm, Sweden |
| authorships[2].author.id | https://openalex.org/A5027642721 |
| authorships[2].author.orcid | |
| authorships[2].author.display_name | Abhilash Kulkarni |
| authorships[2].countries | SE |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I86987016 |
| authorships[2].affiliations[0].raw_affiliation_string | Royal Institute of Technology (KTH), Experimental Biomolecular Physics, Dept. Applied Physics, Albanova University Center, 106 91 Stockholm, Sweden |
| authorships[2].institutions[0].id | https://openalex.org/I86987016 |
| authorships[2].institutions[0].ror | https://ror.org/026vcq606 |
| authorships[2].institutions[0].type | education |
| authorships[2].institutions[0].lineage | https://openalex.org/I86987016 |
| authorships[2].institutions[0].country_code | SE |
| authorships[2].institutions[0].display_name | KTH Royal Institute of Technology |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Abhilash Kulkarni |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Royal Institute of Technology (KTH), Experimental Biomolecular Physics, Dept. Applied Physics, Albanova University Center, 106 91 Stockholm, Sweden |
| authorships[3].author.id | https://openalex.org/A5101813400 |
| authorships[3].author.orcid | https://orcid.org/0000-0002-1780-7746 |
| authorships[3].author.display_name | Haichun Liu |
| authorships[3].countries | SE |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I86987016 |
| authorships[3].affiliations[0].raw_affiliation_string | Royal Institute of Technology (KTH), Experimental Biomolecular Physics, Dept. Applied Physics, Albanova University Center, 106 91 Stockholm, Sweden |
| authorships[3].institutions[0].id | https://openalex.org/I86987016 |
| authorships[3].institutions[0].ror | https://ror.org/026vcq606 |
| authorships[3].institutions[0].type | education |
| authorships[3].institutions[0].lineage | https://openalex.org/I86987016 |
| authorships[3].institutions[0].country_code | SE |
| authorships[3].institutions[0].display_name | KTH Royal Institute of Technology |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Haichun Liu |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | Royal Institute of Technology (KTH), Experimental Biomolecular Physics, Dept. Applied Physics, Albanova University Center, 106 91 Stockholm, Sweden |
| authorships[4].author.id | https://openalex.org/A5025699533 |
| authorships[4].author.orcid | https://orcid.org/0000-0002-4762-4887 |
| authorships[4].author.display_name | Joachim Piguet |
| authorships[4].countries | SE |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I86987016 |
| authorships[4].affiliations[0].raw_affiliation_string | Royal Institute of Technology (KTH), Experimental Biomolecular Physics, Dept. Applied Physics, Albanova University Center, 106 91 Stockholm, Sweden |
| authorships[4].institutions[0].id | https://openalex.org/I86987016 |
| authorships[4].institutions[0].ror | https://ror.org/026vcq606 |
| authorships[4].institutions[0].type | education |
| authorships[4].institutions[0].lineage | https://openalex.org/I86987016 |
| authorships[4].institutions[0].country_code | SE |
| authorships[4].institutions[0].display_name | KTH Royal Institute of Technology |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Joachim Piguet |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | Royal Institute of Technology (KTH), Experimental Biomolecular Physics, Dept. Applied Physics, Albanova University Center, 106 91 Stockholm, Sweden |
| authorships[5].author.id | https://openalex.org/A5013002722 |
| authorships[5].author.orcid | https://orcid.org/0000-0003-3200-0374 |
| authorships[5].author.display_name | Jerker Widengren |
| authorships[5].countries | SE |
| authorships[5].affiliations[0].institution_ids | https://openalex.org/I86987016 |
| authorships[5].affiliations[0].raw_affiliation_string | Royal Institute of Technology (KTH), Experimental Biomolecular Physics, Dept. Applied Physics, Albanova University Center, 106 91 Stockholm, Sweden |
| authorships[5].institutions[0].id | https://openalex.org/I86987016 |
| authorships[5].institutions[0].ror | https://ror.org/026vcq606 |
| authorships[5].institutions[0].type | education |
| authorships[5].institutions[0].lineage | https://openalex.org/I86987016 |
| authorships[5].institutions[0].country_code | SE |
| authorships[5].institutions[0].display_name | KTH Royal Institute of Technology |
| authorships[5].author_position | last |
| authorships[5].raw_author_name | Jerker Widengren |
| authorships[5].is_corresponding | True |
| authorships[5].raw_affiliation_strings | Royal Institute of Technology (KTH), Experimental Biomolecular Physics, Dept. Applied Physics, Albanova University Center, 106 91 Stockholm, Sweden |
| has_content.pdf | False |
| has_content.grobid_xml | False |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://doi.org/10.1021/acs.jpcb.3c06905 |
| open_access.oa_status | hybrid |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Fluorescence Bar-Coding and Flowmetry Based on Dark State Transitions in Fluorescence Emitters |
| has_fulltext | False |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T10540 |
| primary_topic.field.id | https://openalex.org/fields/13 |
| primary_topic.field.display_name | Biochemistry, Genetics and Molecular Biology |
| primary_topic.score | 0.9937000274658203 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1304 |
| primary_topic.subfield.display_name | Biophysics |
| primary_topic.display_name | Advanced Fluorescence Microscopy Techniques |
| related_works | https://openalex.org/W4246450666, https://openalex.org/W4388998267, https://openalex.org/W2898370298, https://openalex.org/W2137437058, https://openalex.org/W4390401159, https://openalex.org/W2744391499, https://openalex.org/W3120461830, https://openalex.org/W4230250635, https://openalex.org/W3041790586, https://openalex.org/W2018879842 |
| cited_by_count | 6 |
| counts_by_year[0].year | 2025 |
| counts_by_year[0].cited_by_count | 5 |
| counts_by_year[1].year | 2024 |
| counts_by_year[1].cited_by_count | 1 |
| locations_count | 3 |
| best_oa_location.id | doi:10.1021/acs.jpcb.3c06905 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S185621672 |
| best_oa_location.source.issn | 1520-5207, 1520-6106 |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | False |
| best_oa_location.source.issn_l | 1520-5207 |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | False |
| best_oa_location.source.display_name | The Journal of Physical Chemistry B |
| best_oa_location.source.host_organization | https://openalex.org/P4310320006 |
| best_oa_location.source.host_organization_name | American Chemical Society |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310320006 |
| best_oa_location.source.host_organization_lineage_names | American Chemical Society |
| best_oa_location.license | cc-by |
| best_oa_location.pdf_url | |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | The Journal of Physical Chemistry B |
| best_oa_location.landing_page_url | https://doi.org/10.1021/acs.jpcb.3c06905 |
| primary_location.id | doi:10.1021/acs.jpcb.3c06905 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S185621672 |
| primary_location.source.issn | 1520-5207, 1520-6106 |
| primary_location.source.type | journal |
| primary_location.source.is_oa | False |
| primary_location.source.issn_l | 1520-5207 |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | False |
| primary_location.source.display_name | The Journal of Physical Chemistry B |
| primary_location.source.host_organization | https://openalex.org/P4310320006 |
| primary_location.source.host_organization_name | American Chemical Society |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310320006 |
| primary_location.source.host_organization_lineage_names | American Chemical Society |
| primary_location.license | cc-by |
| primary_location.pdf_url | |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | https://openalex.org/licenses/cc-by |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | The Journal of Physical Chemistry B |
| primary_location.landing_page_url | https://doi.org/10.1021/acs.jpcb.3c06905 |
| publication_date | 2023-12-21 |
| publication_year | 2023 |
| referenced_works | https://openalex.org/W2913210443, https://openalex.org/W3216963533, https://openalex.org/W1995830102, https://openalex.org/W2069813722, https://openalex.org/W2315779173, https://openalex.org/W3181819709, https://openalex.org/W2921080611, https://openalex.org/W2995985926, https://openalex.org/W2050538415, https://openalex.org/W4241551375, https://openalex.org/W4205662962, https://openalex.org/W1995417258, https://openalex.org/W1976786825, https://openalex.org/W1986578141, https://openalex.org/W2169791141, https://openalex.org/W2165548215, https://openalex.org/W4294152276, https://openalex.org/W4281636402, https://openalex.org/W4313545894, https://openalex.org/W2068998399, https://openalex.org/W1994149778, https://openalex.org/W4289546506, https://openalex.org/W2049126768, https://openalex.org/W4223925042, https://openalex.org/W2980996914, https://openalex.org/W2991565823, https://openalex.org/W2012379620, https://openalex.org/W2770434908, https://openalex.org/W2002173240, https://openalex.org/W2017795945, https://openalex.org/W2021634744, https://openalex.org/W2118844567, https://openalex.org/W1972470810, https://openalex.org/W2080701341, https://openalex.org/W3039396647, https://openalex.org/W2330710553, https://openalex.org/W3022157795, https://openalex.org/W1968940874, https://openalex.org/W4312223208, https://openalex.org/W2021877333, https://openalex.org/W2063623305, https://openalex.org/W4206998099 |
| referenced_works_count | 42 |
| abstract_inverted_index.a | 7, 15, 44, 109, 146, 164, 190, 206, 244 |
| abstract_inverted_index.In | 32, 193 |
| abstract_inverted_index.an | 232 |
| abstract_inverted_index.as | 43, 150, 152 |
| abstract_inverted_index.be | 79, 143, 184, 241 |
| abstract_inverted_index.by | 85, 96 |
| abstract_inverted_index.in | 4, 10, 108, 153, 227 |
| abstract_inverted_index.is | 211 |
| abstract_inverted_index.of | 40, 66, 122, 134, 158, 173, 247 |
| abstract_inverted_index.on | 64, 145, 201, 243 |
| abstract_inverted_index.or | 205 |
| abstract_inverted_index.to | 195, 215 |
| abstract_inverted_index.we | 35, 162, 220 |
| abstract_inverted_index.Cy5 | 48, 77, 138, 175 |
| abstract_inverted_index.and | 69, 139, 168, 176, 179, 210, 250 |
| abstract_inverted_index.are | 14 |
| abstract_inverted_index.but | 22 |
| abstract_inverted_index.can | 230, 240 |
| abstract_inverted_index.for | 18, 236 |
| abstract_inverted_index.how | 114, 170 |
| abstract_inverted_index.its | 86 |
| abstract_inverted_index.may | 23 |
| abstract_inverted_index.not | 199 |
| abstract_inverted_index.the | 37, 83, 103, 115, 123, 159, 223 |
| abstract_inverted_index.was | 93, 128 |
| abstract_inverted_index.FCS, | 196 |
| abstract_inverted_index.SUVs | 182 |
| abstract_inverted_index.This | 92 |
| abstract_inverted_index.also | 24 |
| abstract_inverted_index.both | 248 |
| abstract_inverted_index.cell | 148 |
| abstract_inverted_index.dark | 1, 105 |
| abstract_inverted_index.does | 198 |
| abstract_inverted_index.free | 67, 174 |
| abstract_inverted_index.from | 49, 82, 113 |
| abstract_inverted_index.high | 207 |
| abstract_inverted_index.live | 135 |
| abstract_inverted_index.more | 110 |
| abstract_inverted_index.next | 94 |
| abstract_inverted_index.rely | 200 |
| abstract_inverted_index.that | 76, 222, 239 |
| abstract_inverted_index.then | 129 |
| abstract_inverted_index.this | 33, 228 |
| abstract_inverted_index.thus | 212 |
| abstract_inverted_index.time | 208 |
| abstract_inverted_index.upon | 120 |
| abstract_inverted_index.well | 151 |
| abstract_inverted_index.when | 187 |
| abstract_inverted_index.with | 131 |
| abstract_inverted_index.work | 229 |
| abstract_inverted_index.(Cy5) | 42 |
| abstract_inverted_index.(FCS) | 61 |
| abstract_inverted_index.TRAST | 127, 166, 197 |
| abstract_inverted_index.basis | 17 |
| abstract_inverted_index.broad | 245 |
| abstract_inverted_index.could | 78, 142, 183 |
| abstract_inverted_index.level | 149 |
| abstract_inverted_index.offer | 25, 231 |
| abstract_inverted_index.range | 246 |
| abstract_inverted_index.small | 71 |
| abstract_inverted_index.state | 2, 98, 106 |
| abstract_inverted_index.whole | 147 |
| abstract_inverted_index.work, | 34 |
| abstract_inverted_index.(SUVs) | 74 |
| abstract_inverted_index.First, | 57 |
| abstract_inverted_index.cells, | 136 |
| abstract_inverted_index.cells. | 160 |
| abstract_inverted_index.expect | 221 |
| abstract_inverted_index.factor | 9 |
| abstract_inverted_index.having | 52 |
| abstract_inverted_index.images | 157 |
| abstract_inverted_index.light. | 126 |
| abstract_inverted_index.moving | 251 |
| abstract_inverted_index.robust | 111 |
| abstract_inverted_index.showed | 75, 169 |
| abstract_inverted_index.useful | 234 |
| abstract_inverted_index.varies | 119 |
| abstract_inverted_index.(TRAST) | 99 |
| abstract_inverted_index.applied | 124 |
| abstract_inverted_index.broadly | 213 |
| abstract_inverted_index.concept | 167, 225 |
| abstract_inverted_index.feature | 46 |
| abstract_inverted_index.imaging | 133 |
| abstract_inverted_index.largely | 27, 53, 88 |
| abstract_inverted_index.manner, | 112 |
| abstract_inverted_index.passing | 188 |
| abstract_inverted_index.readily | 80 |
| abstract_inverted_index.readout | 30 |
| abstract_inverted_index.through | 189 |
| abstract_inverted_index.whereby | 137 |
| abstract_inverted_index.Cyanine5 | 41 |
| abstract_inverted_index.Finally, | 161 |
| abstract_inverted_index.analyzed | 36 |
| abstract_inverted_index.blinking | 38 |
| abstract_inverted_index.channel. | 192 |
| abstract_inverted_index.combined | 130 |
| abstract_inverted_index.contrast | 194 |
| abstract_inverted_index.emission | 55 |
| abstract_inverted_index.imaging, | 21 |
| abstract_inverted_index.kinetics | 39, 107 |
| abstract_inverted_index.limiting | 8 |
| abstract_inverted_index.mixtures | 65, 172 |
| abstract_inverted_index.samples. | 218, 252 |
| abstract_inverted_index.solution | 62 |
| abstract_inverted_index.spectra. | 56 |
| abstract_inverted_index.strategy | 235 |
| abstract_inverted_index.vesicles | 73 |
| abstract_inverted_index.confirmed | 95 |
| abstract_inverted_index.detection | 203 |
| abstract_inverted_index.different | 171, 216 |
| abstract_inverted_index.intensity | 118 |
| abstract_inverted_index.necessary | 16 |
| abstract_inverted_index.presented | 226 |
| abstract_inverted_index.represent | 6 |
| abstract_inverted_index.resolved, | 155 |
| abstract_inverted_index.rhodamine | 50, 140, 177 |
| abstract_inverted_index.spatially | 154 |
| abstract_inverted_index.transient | 97 |
| abstract_inverted_index.Reversible | 0 |
| abstract_inverted_index.Therefore, | 219 |
| abstract_inverted_index.additional | 233 |
| abstract_inverted_index.applicable | 214 |
| abstract_inverted_index.bar-coding | 45, 224 |
| abstract_inverted_index.biological | 217 |
| abstract_inverted_index.conditions | 204 |
| abstract_inverted_index.excitation | 125 |
| abstract_inverted_index.modulation | 121 |
| abstract_inverted_index.on-the-fly | 186 |
| abstract_inverted_index.orthogonal | 28 |
| abstract_inverted_index.resolution | 209 |
| abstract_inverted_index.rhodamines | 84 |
| abstract_inverted_index.stationary | 249 |
| abstract_inverted_index.wide-field | 132 |
| abstract_inverted_index.additional, | 26 |
| abstract_inverted_index.correlation | 59 |
| abstract_inverted_index.determining | 102 |
| abstract_inverted_index.established | 163 |
| abstract_inverted_index.fluorophore | 104 |
| abstract_inverted_index.implemented | 242 |
| abstract_inverted_index.multiplexed | 156 |
| abstract_inverted_index.overlapping | 54 |
| abstract_inverted_index.parameters. | 31 |
| abstract_inverted_index.reversible, | 87 |
| abstract_inverted_index.transitions | 3 |
| abstract_inverted_index.unilamellar | 72 |
| abstract_inverted_index.fluorescence | 58, 117 |
| abstract_inverted_index.fluorophores | 5, 51, 68, 141, 178 |
| abstract_inverted_index.measurements | 63 |
| abstract_inverted_index.microfluidic | 165, 191 |
| abstract_inverted_index.multiplexing | 238 |
| abstract_inverted_index.spectroscopy | 60, 100 |
| abstract_inverted_index.corresponding | 180 |
| abstract_inverted_index.distinguished | 81, 144, 185 |
| abstract_inverted_index.measurements, | 101 |
| abstract_inverted_index.spectroscopy, | 13 |
| abstract_inverted_index.time-averaged | 116 |
| abstract_inverted_index.distinguishing | 47 |
| abstract_inverted_index.isomerization. | 91 |
| abstract_inverted_index.ultrasensitive | 12 |
| abstract_inverted_index.single-molecule | 202 |
| abstract_inverted_index.<i>trans-cis</i> | 90 |
| abstract_inverted_index.super-resolution | 20 |
| abstract_inverted_index.excitation-driven | 89 |
| abstract_inverted_index.fluorescence-based | 11, 19, 29, 237 |
| abstract_inverted_index.fluorophore-labeled | 70, 181 |
| cited_by_percentile_year.max | 98 |
| cited_by_percentile_year.min | 90 |
| corresponding_author_ids | https://openalex.org/A5013002722 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 6 |
| corresponding_institution_ids | https://openalex.org/I86987016 |
| sustainable_development_goals[0].id | https://metadata.un.org/sdg/13 |
| sustainable_development_goals[0].score | 0.7099999785423279 |
| sustainable_development_goals[0].display_name | Climate action |
| citation_normalized_percentile.value | 0.9035054 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |