Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications Article Swipe
Rupsa Datta
,
Tiffany M. Heaster
,
Joe T. Sharick
,
Amani A. Gillette
,
Melissa C. Skala
·
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1117/1.jbo.25.7.071203
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1117/1.jbo.25.7.071203
FLIM is advantageous for probing molecular environments of fluorophores to inform on fluorophore behavior that cannot be elucidated with intensity measurements alone. Development of FLIM technologies, analysis, and applications will further advance biological research and clinical assessments.
Related Topics
Concepts
Metadata
- Type
- review
- Language
- en
- Landing Page
- https://doi.org/10.1117/1.jbo.25.7.071203
- https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-25/issue-7/071203/Fluorescence-lifetime-imaging-microscopy--fundamentals-and-advances-in-instrumentation/10.1117/1.JBO.25.7.071203.pdf
- OA Status
- gold
- Cited By
- 755
- References
- 334
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3025520212
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W3025520212Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1117/1.jbo.25.7.071203Digital Object Identifier
- Title
-
Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applicationsWork title
- Type
-
reviewOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2020Year of publication
- Publication date
-
2020-05-13Full publication date if available
- Authors
-
Rupsa Datta, Tiffany M. Heaster, Joe T. Sharick, Amani A. Gillette, Melissa C. SkalaList of authors in order
- Landing page
-
https://doi.org/10.1117/1.jbo.25.7.071203Publisher landing page
- PDF URL
-
https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-25/issue-7/071203/Fluorescence-lifetime-imaging-microscopy--fundamentals-and-advances-in-instrumentation/10.1117/1.JBO.25.7.071203.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-25/issue-7/071203/Fluorescence-lifetime-imaging-microscopy--fundamentals-and-advances-in-instrumentation/10.1117/1.JBO.25.7.071203.pdfDirect OA link when available
- Concepts
-
Fluorophore, Fluorescence-lifetime imaging microscopy, Förster resonance energy transfer, Microscopy, Molecular imaging, Instrumentation (computer programming), Materials science, Nanotechnology, Fluorescence, Computer science, Physics, Optics, Biology, Operating system, In vivo, BiotechnologyTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
755Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 184, 2024: 204, 2023: 147, 2022: 116, 2021: 88Per-year citation counts (last 5 years)
- References (count)
-
334Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W3025520212 |
|---|---|
| doi | https://doi.org/10.1117/1.jbo.25.7.071203 |
| ids.doi | https://doi.org/10.1117/1.jbo.25.7.071203 |
| ids.mag | 3025520212 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/32406215 |
| ids.openalex | https://openalex.org/W3025520212 |
| fwci | 49.75046245 |
| mesh[0].qualifier_ui | |
| mesh[0].descriptor_ui | D004735 |
| mesh[0].is_major_topic | False |
| mesh[0].qualifier_name | |
| mesh[0].descriptor_name | Energy Transfer |
| mesh[1].qualifier_ui | |
| mesh[1].descriptor_ui | D031541 |
| mesh[1].is_major_topic | True |
| mesh[1].qualifier_name | |
| mesh[1].descriptor_name | Fluorescence Resonance Energy Transfer |
| mesh[2].qualifier_ui | |
| mesh[2].descriptor_ui | D005456 |
| mesh[2].is_major_topic | True |
| mesh[2].qualifier_name | |
| mesh[2].descriptor_name | Fluorescent Dyes |
| mesh[3].qualifier_ui | |
| mesh[3].descriptor_ui | D008856 |
| mesh[3].is_major_topic | False |
| mesh[3].qualifier_name | |
| mesh[3].descriptor_name | Microscopy, Fluorescence |
| mesh[4].qualifier_ui | |
| mesh[4].descriptor_ui | D004735 |
| mesh[4].is_major_topic | False |
| mesh[4].qualifier_name | |
| mesh[4].descriptor_name | Energy Transfer |
| mesh[5].qualifier_ui | |
| mesh[5].descriptor_ui | D031541 |
| mesh[5].is_major_topic | True |
| mesh[5].qualifier_name | |
| mesh[5].descriptor_name | Fluorescence Resonance Energy Transfer |
| mesh[6].qualifier_ui | |
| mesh[6].descriptor_ui | D005456 |
| mesh[6].is_major_topic | True |
| mesh[6].qualifier_name | |
| mesh[6].descriptor_name | Fluorescent Dyes |
| mesh[7].qualifier_ui | |
| mesh[7].descriptor_ui | D008856 |
| mesh[7].is_major_topic | False |
| mesh[7].qualifier_name | |
| mesh[7].descriptor_name | Microscopy, Fluorescence |
| mesh[8].qualifier_ui | |
| mesh[8].descriptor_ui | D004735 |
| mesh[8].is_major_topic | False |
| mesh[8].qualifier_name | |
| mesh[8].descriptor_name | Energy Transfer |
| mesh[9].qualifier_ui | |
| mesh[9].descriptor_ui | D031541 |
| mesh[9].is_major_topic | True |
| mesh[9].qualifier_name | |
| mesh[9].descriptor_name | Fluorescence Resonance Energy Transfer |
| mesh[10].qualifier_ui | |
| mesh[10].descriptor_ui | D005456 |
| mesh[10].is_major_topic | True |
| mesh[10].qualifier_name | |
| mesh[10].descriptor_name | Fluorescent Dyes |
| mesh[11].qualifier_ui | |
| mesh[11].descriptor_ui | D008856 |
| mesh[11].is_major_topic | False |
| mesh[11].qualifier_name | |
| mesh[11].descriptor_name | Microscopy, Fluorescence |
| mesh[12].qualifier_ui | |
| mesh[12].descriptor_ui | D004735 |
| mesh[12].is_major_topic | False |
| mesh[12].qualifier_name | |
| mesh[12].descriptor_name | Energy Transfer |
| mesh[13].qualifier_ui | |
| mesh[13].descriptor_ui | D031541 |
| mesh[13].is_major_topic | True |
| mesh[13].qualifier_name | |
| mesh[13].descriptor_name | Fluorescence Resonance Energy Transfer |
| mesh[14].qualifier_ui | |
| mesh[14].descriptor_ui | D005456 |
| mesh[14].is_major_topic | True |
| mesh[14].qualifier_name | |
| mesh[14].descriptor_name | Fluorescent Dyes |
| mesh[15].qualifier_ui | |
| mesh[15].descriptor_ui | D008856 |
| mesh[15].is_major_topic | False |
| mesh[15].qualifier_name | |
| mesh[15].descriptor_name | Microscopy, Fluorescence |
| mesh[16].qualifier_ui | |
| mesh[16].descriptor_ui | D004735 |
| mesh[16].is_major_topic | False |
| mesh[16].qualifier_name | |
| mesh[16].descriptor_name | Energy Transfer |
| mesh[17].qualifier_ui | |
| mesh[17].descriptor_ui | D031541 |
| mesh[17].is_major_topic | True |
| mesh[17].qualifier_name | |
| mesh[17].descriptor_name | Fluorescence Resonance Energy Transfer |
| mesh[18].qualifier_ui | |
| mesh[18].descriptor_ui | D005456 |
| mesh[18].is_major_topic | True |
| mesh[18].qualifier_name | |
| mesh[18].descriptor_name | Fluorescent Dyes |
| mesh[19].qualifier_ui | |
| mesh[19].descriptor_ui | D008856 |
| mesh[19].is_major_topic | False |
| mesh[19].qualifier_name | |
| mesh[19].descriptor_name | Microscopy, Fluorescence |
| type | review |
| title | Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications |
| biblio.issue | 07 |
| biblio.volume | 25 |
| biblio.last_page | 1 |
| biblio.first_page | 1 |
| topics[0].id | https://openalex.org/T10540 |
| topics[0].field.id | https://openalex.org/fields/13 |
| topics[0].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[0].score | 1.0 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1304 |
| topics[0].subfield.display_name | Biophysics |
| topics[0].display_name | Advanced Fluorescence Microscopy Techniques |
| topics[1].id | https://openalex.org/T12015 |
| topics[1].field.id | https://openalex.org/fields/22 |
| topics[1].field.display_name | Engineering |
| topics[1].score | 0.996999979019165 |
| topics[1].domain.id | https://openalex.org/domains/3 |
| topics[1].domain.display_name | Physical Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/2204 |
| topics[1].subfield.display_name | Biomedical Engineering |
| topics[1].display_name | Photoacoustic and Ultrasonic Imaging |
| topics[2].id | https://openalex.org/T10857 |
| topics[2].field.id | https://openalex.org/fields/13 |
| topics[2].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[2].score | 0.9941999912261963 |
| topics[2].domain.id | https://openalex.org/domains/1 |
| topics[2].domain.display_name | Life Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/1315 |
| topics[2].subfield.display_name | Structural Biology |
| topics[2].display_name | Advanced Electron Microscopy Techniques and Applications |
| is_xpac | False |
| apc_list.value | 1675 |
| apc_list.currency | USD |
| apc_list.value_usd | 1675 |
| apc_paid.value | 1675 |
| apc_paid.currency | USD |
| apc_paid.value_usd | 1675 |
| concepts[0].id | https://openalex.org/C2778617687 |
| concepts[0].level | 3 |
| concepts[0].score | 0.7773985862731934 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q2448456 |
| concepts[0].display_name | Fluorophore |
| concepts[1].id | https://openalex.org/C57477423 |
| concepts[1].level | 3 |
| concepts[1].score | 0.7135463953018188 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q1434196 |
| concepts[1].display_name | Fluorescence-lifetime imaging microscopy |
| concepts[2].id | https://openalex.org/C96305047 |
| concepts[2].level | 3 |
| concepts[2].score | 0.6699872016906738 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q367473 |
| concepts[2].display_name | Förster resonance energy transfer |
| concepts[3].id | https://openalex.org/C147080431 |
| concepts[3].level | 2 |
| concepts[3].score | 0.5544504523277283 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q1074953 |
| concepts[3].display_name | Microscopy |
| concepts[4].id | https://openalex.org/C136339569 |
| concepts[4].level | 3 |
| concepts[4].score | 0.47446781396865845 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q1142297 |
| concepts[4].display_name | Molecular imaging |
| concepts[5].id | https://openalex.org/C118530786 |
| concepts[5].level | 2 |
| concepts[5].score | 0.4676506221294403 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q1134732 |
| concepts[5].display_name | Instrumentation (computer programming) |
| concepts[6].id | https://openalex.org/C192562407 |
| concepts[6].level | 0 |
| concepts[6].score | 0.4162635803222656 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q228736 |
| concepts[6].display_name | Materials science |
| concepts[7].id | https://openalex.org/C171250308 |
| concepts[7].level | 1 |
| concepts[7].score | 0.4030812382698059 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q11468 |
| concepts[7].display_name | Nanotechnology |
| concepts[8].id | https://openalex.org/C91881484 |
| concepts[8].level | 2 |
| concepts[8].score | 0.3616626262664795 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q191807 |
| concepts[8].display_name | Fluorescence |
| concepts[9].id | https://openalex.org/C41008148 |
| concepts[9].level | 0 |
| concepts[9].score | 0.24586382508277893 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q21198 |
| concepts[9].display_name | Computer science |
| concepts[10].id | https://openalex.org/C121332964 |
| concepts[10].level | 0 |
| concepts[10].score | 0.21006852388381958 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q413 |
| concepts[10].display_name | Physics |
| concepts[11].id | https://openalex.org/C120665830 |
| concepts[11].level | 1 |
| concepts[11].score | 0.20290923118591309 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q14620 |
| concepts[11].display_name | Optics |
| concepts[12].id | https://openalex.org/C86803240 |
| concepts[12].level | 0 |
| concepts[12].score | 0.11170810461044312 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[12].display_name | Biology |
| concepts[13].id | https://openalex.org/C111919701 |
| concepts[13].level | 1 |
| concepts[13].score | 0.0 |
| concepts[13].wikidata | https://www.wikidata.org/wiki/Q9135 |
| concepts[13].display_name | Operating system |
| concepts[14].id | https://openalex.org/C207001950 |
| concepts[14].level | 2 |
| concepts[14].score | 0.0 |
| concepts[14].wikidata | https://www.wikidata.org/wiki/Q141124 |
| concepts[14].display_name | In vivo |
| concepts[15].id | https://openalex.org/C150903083 |
| concepts[15].level | 1 |
| concepts[15].score | 0.0 |
| concepts[15].wikidata | https://www.wikidata.org/wiki/Q7108 |
| concepts[15].display_name | Biotechnology |
| keywords[0].id | https://openalex.org/keywords/fluorophore |
| keywords[0].score | 0.7773985862731934 |
| keywords[0].display_name | Fluorophore |
| keywords[1].id | https://openalex.org/keywords/fluorescence-lifetime-imaging-microscopy |
| keywords[1].score | 0.7135463953018188 |
| keywords[1].display_name | Fluorescence-lifetime imaging microscopy |
| keywords[2].id | https://openalex.org/keywords/förster-resonance-energy-transfer |
| keywords[2].score | 0.6699872016906738 |
| keywords[2].display_name | Förster resonance energy transfer |
| keywords[3].id | https://openalex.org/keywords/microscopy |
| keywords[3].score | 0.5544504523277283 |
| keywords[3].display_name | Microscopy |
| keywords[4].id | https://openalex.org/keywords/molecular-imaging |
| keywords[4].score | 0.47446781396865845 |
| keywords[4].display_name | Molecular imaging |
| keywords[5].id | https://openalex.org/keywords/instrumentation |
| keywords[5].score | 0.4676506221294403 |
| keywords[5].display_name | Instrumentation (computer programming) |
| keywords[6].id | https://openalex.org/keywords/materials-science |
| keywords[6].score | 0.4162635803222656 |
| keywords[6].display_name | Materials science |
| keywords[7].id | https://openalex.org/keywords/nanotechnology |
| keywords[7].score | 0.4030812382698059 |
| keywords[7].display_name | Nanotechnology |
| keywords[8].id | https://openalex.org/keywords/fluorescence |
| keywords[8].score | 0.3616626262664795 |
| keywords[8].display_name | Fluorescence |
| keywords[9].id | https://openalex.org/keywords/computer-science |
| keywords[9].score | 0.24586382508277893 |
| keywords[9].display_name | Computer science |
| keywords[10].id | https://openalex.org/keywords/physics |
| keywords[10].score | 0.21006852388381958 |
| keywords[10].display_name | Physics |
| keywords[11].id | https://openalex.org/keywords/optics |
| keywords[11].score | 0.20290923118591309 |
| keywords[11].display_name | Optics |
| keywords[12].id | https://openalex.org/keywords/biology |
| keywords[12].score | 0.11170810461044312 |
| keywords[12].display_name | Biology |
| language | en |
| locations[0].id | doi:10.1117/1.jbo.25.7.071203 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S117354312 |
| locations[0].source.issn | 1083-3668, 1560-2281 |
| locations[0].source.type | journal |
| locations[0].source.is_oa | True |
| locations[0].source.issn_l | 1083-3668 |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | True |
| locations[0].source.display_name | Journal of Biomedical Optics |
| locations[0].source.host_organization | https://openalex.org/P4310315543 |
| locations[0].source.host_organization_name | SPIE |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310315543 |
| locations[0].source.host_organization_lineage_names | SPIE |
| locations[0].license | cc-by |
| locations[0].pdf_url | https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-25/issue-7/071203/Fluorescence-lifetime-imaging-microscopy--fundamentals-and-advances-in-instrumentation/10.1117/1.JBO.25.7.071203.pdf |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | https://openalex.org/licenses/cc-by |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | Journal of Biomedical Optics |
| locations[0].landing_page_url | https://doi.org/10.1117/1.jbo.25.7.071203 |
| locations[1].id | pmid:32406215 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | Journal of biomedical optics |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/32406215 |
| locations[2].id | pmh:oai:pubmedcentral.nih.gov:7219965 |
| locations[2].is_oa | True |
| locations[2].source.id | https://openalex.org/S2764455111 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | PubMed Central |
| locations[2].source.host_organization | https://openalex.org/I1299303238 |
| locations[2].source.host_organization_name | National Institutes of Health |
| locations[2].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[2].license | cc-by |
| locations[2].pdf_url | |
| locations[2].version | submittedVersion |
| locations[2].raw_type | Text |
| locations[2].license_id | https://openalex.org/licenses/cc-by |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | J Biomed Opt |
| locations[2].landing_page_url | https://www.ncbi.nlm.nih.gov/pmc/articles/7219965 |
| indexed_in | crossref, doaj, pubmed |
| authorships[0].author.id | https://openalex.org/A5083395619 |
| authorships[0].author.orcid | https://orcid.org/0000-0002-6432-2389 |
| authorships[0].author.display_name | Rupsa Datta |
| authorships[0].countries | US |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I29680605 |
| authorships[0].affiliations[0].raw_affiliation_string | Morgridge Institute for Research, Madison, Wisconsin |
| authorships[0].institutions[0].id | https://openalex.org/I29680605 |
| authorships[0].institutions[0].ror | https://ror.org/05cb4rb43 |
| authorships[0].institutions[0].type | nonprofit |
| authorships[0].institutions[0].lineage | https://openalex.org/I135310074, https://openalex.org/I29680605 |
| authorships[0].institutions[0].country_code | US |
| authorships[0].institutions[0].display_name | Morgridge Institute for Research |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Rupsa Datta |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | Morgridge Institute for Research, Madison, Wisconsin |
| authorships[1].author.id | https://openalex.org/A5008261654 |
| authorships[1].author.orcid | https://orcid.org/0000-0001-9454-9392 |
| authorships[1].author.display_name | Tiffany M. Heaster |
| authorships[1].countries | US |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I135310074 |
| authorships[1].affiliations[0].raw_affiliation_string | Univ. of Wisconsin-Madison (United States) |
| authorships[1].affiliations[1].institution_ids | https://openalex.org/I29680605 |
| authorships[1].affiliations[1].raw_affiliation_string | Morgridge Institute for Research, Madison, Wisconsin |
| authorships[1].institutions[0].id | https://openalex.org/I29680605 |
| authorships[1].institutions[0].ror | https://ror.org/05cb4rb43 |
| authorships[1].institutions[0].type | nonprofit |
| authorships[1].institutions[0].lineage | https://openalex.org/I135310074, https://openalex.org/I29680605 |
| authorships[1].institutions[0].country_code | US |
| authorships[1].institutions[0].display_name | Morgridge Institute for Research |
| authorships[1].institutions[1].id | https://openalex.org/I135310074 |
| authorships[1].institutions[1].ror | https://ror.org/01y2jtd41 |
| authorships[1].institutions[1].type | education |
| authorships[1].institutions[1].lineage | https://openalex.org/I135310074 |
| authorships[1].institutions[1].country_code | US |
| authorships[1].institutions[1].display_name | University of Wisconsin–Madison |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Tiffany M. Heaster |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Morgridge Institute for Research, Madison, Wisconsin, Univ. of Wisconsin-Madison (United States) |
| authorships[2].author.id | https://openalex.org/A5065781899 |
| authorships[2].author.orcid | https://orcid.org/0000-0002-1517-2062 |
| authorships[2].author.display_name | Joe T. Sharick |
| authorships[2].countries | US |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I29680605 |
| authorships[2].affiliations[0].raw_affiliation_string | Morgridge Institute for Research, Madison, Wisconsin |
| authorships[2].institutions[0].id | https://openalex.org/I29680605 |
| authorships[2].institutions[0].ror | https://ror.org/05cb4rb43 |
| authorships[2].institutions[0].type | nonprofit |
| authorships[2].institutions[0].lineage | https://openalex.org/I135310074, https://openalex.org/I29680605 |
| authorships[2].institutions[0].country_code | US |
| authorships[2].institutions[0].display_name | Morgridge Institute for Research |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Joe T. Sharick |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Morgridge Institute for Research, Madison, Wisconsin |
| authorships[3].author.id | https://openalex.org/A5049773860 |
| authorships[3].author.orcid | https://orcid.org/0000-0002-1470-3300 |
| authorships[3].author.display_name | Amani A. Gillette |
| authorships[3].countries | US |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I29680605 |
| authorships[3].affiliations[0].raw_affiliation_string | Morgridge Institute for Research, Madison, Wisconsin |
| authorships[3].affiliations[1].institution_ids | https://openalex.org/I135310074 |
| authorships[3].affiliations[1].raw_affiliation_string | Univ. of Wisconsin-Madison (United States) |
| authorships[3].institutions[0].id | https://openalex.org/I29680605 |
| authorships[3].institutions[0].ror | https://ror.org/05cb4rb43 |
| authorships[3].institutions[0].type | nonprofit |
| authorships[3].institutions[0].lineage | https://openalex.org/I135310074, https://openalex.org/I29680605 |
| authorships[3].institutions[0].country_code | US |
| authorships[3].institutions[0].display_name | Morgridge Institute for Research |
| authorships[3].institutions[1].id | https://openalex.org/I135310074 |
| authorships[3].institutions[1].ror | https://ror.org/01y2jtd41 |
| authorships[3].institutions[1].type | education |
| authorships[3].institutions[1].lineage | https://openalex.org/I135310074 |
| authorships[3].institutions[1].country_code | US |
| authorships[3].institutions[1].display_name | University of Wisconsin–Madison |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Amani A. Gillette |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | Morgridge Institute for Research, Madison, Wisconsin, Univ. of Wisconsin-Madison (United States) |
| authorships[4].author.id | https://openalex.org/A5084628898 |
| authorships[4].author.orcid | https://orcid.org/0000-0002-6320-7637 |
| authorships[4].author.display_name | Melissa C. Skala |
| authorships[4].countries | US |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I29680605 |
| authorships[4].affiliations[0].raw_affiliation_string | Morgridge Institute for Research, Madison, Wisconsin |
| authorships[4].affiliations[1].institution_ids | https://openalex.org/I135310074 |
| authorships[4].affiliations[1].raw_affiliation_string | Univ. of Wisconsin-Madison (United States) |
| authorships[4].institutions[0].id | https://openalex.org/I29680605 |
| authorships[4].institutions[0].ror | https://ror.org/05cb4rb43 |
| authorships[4].institutions[0].type | nonprofit |
| authorships[4].institutions[0].lineage | https://openalex.org/I135310074, https://openalex.org/I29680605 |
| authorships[4].institutions[0].country_code | US |
| authorships[4].institutions[0].display_name | Morgridge Institute for Research |
| authorships[4].institutions[1].id | https://openalex.org/I135310074 |
| authorships[4].institutions[1].ror | https://ror.org/01y2jtd41 |
| authorships[4].institutions[1].type | education |
| authorships[4].institutions[1].lineage | https://openalex.org/I135310074 |
| authorships[4].institutions[1].country_code | US |
| authorships[4].institutions[1].display_name | University of Wisconsin–Madison |
| authorships[4].author_position | last |
| authorships[4].raw_author_name | Melissa C. Skala |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | Morgridge Institute for Research, Madison, Wisconsin, Univ. of Wisconsin-Madison (United States) |
| has_content.pdf | True |
| has_content.grobid_xml | True |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-25/issue-7/071203/Fluorescence-lifetime-imaging-microscopy--fundamentals-and-advances-in-instrumentation/10.1117/1.JBO.25.7.071203.pdf |
| open_access.oa_status | gold |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications |
| has_fulltext | True |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T10540 |
| primary_topic.field.id | https://openalex.org/fields/13 |
| primary_topic.field.display_name | Biochemistry, Genetics and Molecular Biology |
| primary_topic.score | 1.0 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1304 |
| primary_topic.subfield.display_name | Biophysics |
| primary_topic.display_name | Advanced Fluorescence Microscopy Techniques |
| related_works | https://openalex.org/W2352913266, https://openalex.org/W2075324784, https://openalex.org/W2765800694, https://openalex.org/W2912638874, https://openalex.org/W4232258163, https://openalex.org/W2040923823, https://openalex.org/W2404819898, https://openalex.org/W2035553444, https://openalex.org/W4200203554, https://openalex.org/W2076283703 |
| cited_by_count | 755 |
| counts_by_year[0].year | 2025 |
| counts_by_year[0].cited_by_count | 184 |
| counts_by_year[1].year | 2024 |
| counts_by_year[1].cited_by_count | 204 |
| counts_by_year[2].year | 2023 |
| counts_by_year[2].cited_by_count | 147 |
| counts_by_year[3].year | 2022 |
| counts_by_year[3].cited_by_count | 116 |
| counts_by_year[4].year | 2021 |
| counts_by_year[4].cited_by_count | 88 |
| counts_by_year[5].year | 2020 |
| counts_by_year[5].cited_by_count | 14 |
| counts_by_year[6].year | 2019 |
| counts_by_year[6].cited_by_count | 1 |
| counts_by_year[7].year | 2013 |
| counts_by_year[7].cited_by_count | 1 |
| locations_count | 3 |
| best_oa_location.id | doi:10.1117/1.jbo.25.7.071203 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S117354312 |
| best_oa_location.source.issn | 1083-3668, 1560-2281 |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | True |
| best_oa_location.source.issn_l | 1083-3668 |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | True |
| best_oa_location.source.display_name | Journal of Biomedical Optics |
| best_oa_location.source.host_organization | https://openalex.org/P4310315543 |
| best_oa_location.source.host_organization_name | SPIE |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310315543 |
| best_oa_location.source.host_organization_lineage_names | SPIE |
| best_oa_location.license | cc-by |
| best_oa_location.pdf_url | https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-25/issue-7/071203/Fluorescence-lifetime-imaging-microscopy--fundamentals-and-advances-in-instrumentation/10.1117/1.JBO.25.7.071203.pdf |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | Journal of Biomedical Optics |
| best_oa_location.landing_page_url | https://doi.org/10.1117/1.jbo.25.7.071203 |
| primary_location.id | doi:10.1117/1.jbo.25.7.071203 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S117354312 |
| primary_location.source.issn | 1083-3668, 1560-2281 |
| primary_location.source.type | journal |
| primary_location.source.is_oa | True |
| primary_location.source.issn_l | 1083-3668 |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | True |
| primary_location.source.display_name | Journal of Biomedical Optics |
| primary_location.source.host_organization | https://openalex.org/P4310315543 |
| primary_location.source.host_organization_name | SPIE |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310315543 |
| primary_location.source.host_organization_lineage_names | SPIE |
| primary_location.license | cc-by |
| primary_location.pdf_url | https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-25/issue-7/071203/Fluorescence-lifetime-imaging-microscopy--fundamentals-and-advances-in-instrumentation/10.1117/1.JBO.25.7.071203.pdf |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | https://openalex.org/licenses/cc-by |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | Journal of Biomedical Optics |
| primary_location.landing_page_url | https://doi.org/10.1117/1.jbo.25.7.071203 |
| publication_date | 2020-05-13 |
| publication_year | 2020 |
| referenced_works | https://openalex.org/W6783596070, https://openalex.org/W2032579724, https://openalex.org/W2162559145, https://openalex.org/W2625525216, https://openalex.org/W2604318464, https://openalex.org/W2134479430, https://openalex.org/W2024901091, https://openalex.org/W2088341766, https://openalex.org/W2922181242, https://openalex.org/W2890600951, https://openalex.org/W2061464027, https://openalex.org/W2320971091, https://openalex.org/W2915478595, https://openalex.org/W6647456094, https://openalex.org/W2146189740, https://openalex.org/W6727112767, https://openalex.org/W2971311915, https://openalex.org/W1632085521, https://openalex.org/W2005757429, https://openalex.org/W2586848680, https://openalex.org/W2077879155, https://openalex.org/W1989568798, https://openalex.org/W2087294008, https://openalex.org/W2806277779, https://openalex.org/W1508618954, https://openalex.org/W6633515835, https://openalex.org/W2085446806, https://openalex.org/W2069562633, https://openalex.org/W7073686817, https://openalex.org/W2156099567, https://openalex.org/W1991321249, https://openalex.org/W1991437967, https://openalex.org/W2095763279, https://openalex.org/W2123039374, https://openalex.org/W1966746445, https://openalex.org/W2185136965, https://openalex.org/W2093850810, https://openalex.org/W1892152977, https://openalex.org/W1864744261, https://openalex.org/W2078145801, https://openalex.org/W2043720672, https://openalex.org/W2159916641, https://openalex.org/W2054170942, https://openalex.org/W2135174215, https://openalex.org/W2588194993, https://openalex.org/W6808226651, https://openalex.org/W2102914553, https://openalex.org/W2035785520, https://openalex.org/W2168080864, https://openalex.org/W1497848855, https://openalex.org/W2059536603, https://openalex.org/W1998934555, https://openalex.org/W2050226576, https://openalex.org/W2158312912, https://openalex.org/W2010451842, https://openalex.org/W2054802737, https://openalex.org/W1965998263, https://openalex.org/W2739112059, https://openalex.org/W1551325117, https://openalex.org/W2904954576, https://openalex.org/W2890630078, https://openalex.org/W3025063128, https://openalex.org/W2342292734, https://openalex.org/W2174367994, https://openalex.org/W2810634200, https://openalex.org/W2898040512, https://openalex.org/W1960176772, https://openalex.org/W2171895487, https://openalex.org/W2156059455, https://openalex.org/W1487368795, https://openalex.org/W2951309741, https://openalex.org/W2338693367, https://openalex.org/W1914776769, https://openalex.org/W2775532164, https://openalex.org/W3006588023, https://openalex.org/W1995783329, https://openalex.org/W2103019383, https://openalex.org/W2154329137, https://openalex.org/W2969408221, https://openalex.org/W2100755157, https://openalex.org/W2479232548, https://openalex.org/W2044625433, https://openalex.org/W2086309281, https://openalex.org/W1548141905, https://openalex.org/W1685372453, https://openalex.org/W2040345348, https://openalex.org/W2051490559, https://openalex.org/W2151614537, https://openalex.org/W2018056590, https://openalex.org/W1981244831, https://openalex.org/W1981362247, https://openalex.org/W2150078661, https://openalex.org/W2106681056, https://openalex.org/W2043495616, https://openalex.org/W2129078957, https://openalex.org/W2031160934, https://openalex.org/W1980046300, https://openalex.org/W2010394440, https://openalex.org/W2957037929, https://openalex.org/W2953660774, https://openalex.org/W2003369214, https://openalex.org/W1970000022, https://openalex.org/W2000381358, https://openalex.org/W1963514012, https://openalex.org/W6604598995, https://openalex.org/W2052650750, https://openalex.org/W2168529896, https://openalex.org/W6764804687, https://openalex.org/W1994198473, https://openalex.org/W2005893031, https://openalex.org/W1992624237, https://openalex.org/W2979475916, https://openalex.org/W1979171396, https://openalex.org/W6749513713, https://openalex.org/W2045143015, https://openalex.org/W2909276764, https://openalex.org/W2974325639, https://openalex.org/W2557891042, https://openalex.org/W2004803144, https://openalex.org/W2026026462, https://openalex.org/W2010709858, https://openalex.org/W2147229775, https://openalex.org/W2050521993, https://openalex.org/W2586166506, https://openalex.org/W2043023303, https://openalex.org/W2146011638, https://openalex.org/W2028254680, https://openalex.org/W2954949498, https://openalex.org/W1493912143, https://openalex.org/W2900502405, https://openalex.org/W2980166556, https://openalex.org/W2958054774, https://openalex.org/W6682093387, https://openalex.org/W2320025761, https://openalex.org/W6808454354, https://openalex.org/W2061464631, https://openalex.org/W2141676709, https://openalex.org/W1581911403, https://openalex.org/W1848876469, https://openalex.org/W2999704872, https://openalex.org/W2915949209, https://openalex.org/W2219356025, https://openalex.org/W2209978129, https://openalex.org/W2598850685, https://openalex.org/W2339403333, https://openalex.org/W2092293413, https://openalex.org/W6688140527, https://openalex.org/W2069022905, https://openalex.org/W1980759701, https://openalex.org/W1964867265, https://openalex.org/W7073919679, https://openalex.org/W2111609795, https://openalex.org/W2472211481, https://openalex.org/W2979126676, https://openalex.org/W2889971728, https://openalex.org/W2751953673, https://openalex.org/W2086356224, https://openalex.org/W2026544751, https://openalex.org/W1608061755, https://openalex.org/W2345992902, https://openalex.org/W2094859955, https://openalex.org/W2086247286, https://openalex.org/W995681685, https://openalex.org/W2066054282, https://openalex.org/W2912986577, https://openalex.org/W2461667943, https://openalex.org/W2901512692, https://openalex.org/W2023215494, https://openalex.org/W2171992932, https://openalex.org/W2129626077, https://openalex.org/W2132835493, https://openalex.org/W2981332173, https://openalex.org/W1971495237, https://openalex.org/W2028734700, https://openalex.org/W942022383, https://openalex.org/W2090973240, https://openalex.org/W1818089369, https://openalex.org/W2009014044, https://openalex.org/W2011024947, https://openalex.org/W2091209961, https://openalex.org/W2799843241, https://openalex.org/W2985938182, https://openalex.org/W2919354217, https://openalex.org/W2896128207, https://openalex.org/W2036925559, https://openalex.org/W2039552793, https://openalex.org/W2938616354, https://openalex.org/W2337277441, https://openalex.org/W2952190369, https://openalex.org/W2912072656, https://openalex.org/W3122872465, https://openalex.org/W2765916628, https://openalex.org/W2964489651, https://openalex.org/W1982478101, https://openalex.org/W2765229307, https://openalex.org/W1901129140, https://openalex.org/W2565639579, https://openalex.org/W6735463952, https://openalex.org/W2980998394, https://openalex.org/W2093389662, https://openalex.org/W2601810315, https://openalex.org/W2160041854, https://openalex.org/W2078091226, https://openalex.org/W2192927701, https://openalex.org/W2944159612, https://openalex.org/W2118727666, https://openalex.org/W6681236446, https://openalex.org/W2028783664, https://openalex.org/W2127403997, https://openalex.org/W2981747912, https://openalex.org/W2165835468, https://openalex.org/W2121388227, https://openalex.org/W2594828440, https://openalex.org/W2586077874, https://openalex.org/W2186740209, https://openalex.org/W2037276225, https://openalex.org/W2919201779, https://openalex.org/W2346165677, https://openalex.org/W2128801127, https://openalex.org/W2112246628, https://openalex.org/W2915117202, https://openalex.org/W2971899259, https://openalex.org/W2332364796, https://openalex.org/W2029214058, https://openalex.org/W2606602047, https://openalex.org/W2503953104, https://openalex.org/W2594792468, https://openalex.org/W2901368560, https://openalex.org/W2887935569, https://openalex.org/W2342841536, https://openalex.org/W2613146192, https://openalex.org/W2791968311, https://openalex.org/W2914958900, https://openalex.org/W1996841581, https://openalex.org/W2015072959, https://openalex.org/W2896776053, https://openalex.org/W2406448959, https://openalex.org/W2083147890, https://openalex.org/W2048151651, https://openalex.org/W2508457726, https://openalex.org/W2287250553, https://openalex.org/W2902226632, https://openalex.org/W2801007349, https://openalex.org/W6732013618, https://openalex.org/W2314492301, https://openalex.org/W2948496414, https://openalex.org/W2898248821, https://openalex.org/W2021888488, https://openalex.org/W2331553323, https://openalex.org/W2971770892, https://openalex.org/W2557294146, https://openalex.org/W2800700891, https://openalex.org/W2084827054, https://openalex.org/W2220382232, https://openalex.org/W2020666373, https://openalex.org/W2004956687, https://openalex.org/W2073295788, https://openalex.org/W2511223728, https://openalex.org/W2106467306, https://openalex.org/W1979935893, https://openalex.org/W2924566864, https://openalex.org/W2275846176, https://openalex.org/W2044849602, https://openalex.org/W2912696061, https://openalex.org/W2269978004, https://openalex.org/W2298361824, https://openalex.org/W2028972427, https://openalex.org/W2056850877, https://openalex.org/W2738496305, https://openalex.org/W2233022199, https://openalex.org/W1481249341, https://openalex.org/W2906918488, https://openalex.org/W2916292935, https://openalex.org/W2411473353, https://openalex.org/W2882115054, https://openalex.org/W90044761, https://openalex.org/W2021186660, https://openalex.org/W2119795324, https://openalex.org/W2034121262, https://openalex.org/W3000660423, https://openalex.org/W2530859476, https://openalex.org/W2410278926, https://openalex.org/W2286284214, https://openalex.org/W2113681822, https://openalex.org/W2910725484, https://openalex.org/W3021590739, https://openalex.org/W113373386, https://openalex.org/W2973538687, https://openalex.org/W2089663038, https://openalex.org/W3002463486, https://openalex.org/W1988505305, https://openalex.org/W2073361681, https://openalex.org/W95925309, https://openalex.org/W2007893458, https://openalex.org/W4387989882, https://openalex.org/W2791794321, https://openalex.org/W4256450049, https://openalex.org/W2070610153, https://openalex.org/W4379510236, https://openalex.org/W4230350617, https://openalex.org/W2562409163, https://openalex.org/W1588445962, https://openalex.org/W2482772788, https://openalex.org/W1561188729, https://openalex.org/W2903467603, https://openalex.org/W2952153001, https://openalex.org/W2133990480, https://openalex.org/W2009110799, https://openalex.org/W2410641963, https://openalex.org/W2175705688, https://openalex.org/W2982440239, https://openalex.org/W2560690681, https://openalex.org/W771328121, https://openalex.org/W4241765435, https://openalex.org/W2177540718, https://openalex.org/W2502654576, https://openalex.org/W2572957424, https://openalex.org/W2140920568, https://openalex.org/W2093527373, https://openalex.org/W2038034321, https://openalex.org/W2903724320, https://openalex.org/W2963150697, https://openalex.org/W2980869058, https://openalex.org/W1877374258, https://openalex.org/W2129827453, https://openalex.org/W4299496276, https://openalex.org/W1922930316, https://openalex.org/W1484258617, https://openalex.org/W2021807814, https://openalex.org/W4211238694, https://openalex.org/W2972467063, https://openalex.org/W2209423014, https://openalex.org/W1561497800, https://openalex.org/W2519425563 |
| referenced_works_count | 334 |
| abstract_inverted_index.be | 16 |
| abstract_inverted_index.is | 1 |
| abstract_inverted_index.of | 7, 23 |
| abstract_inverted_index.on | 11 |
| abstract_inverted_index.to | 9 |
| abstract_inverted_index.and | 27, 34 |
| abstract_inverted_index.for | 3 |
| abstract_inverted_index.FLIM | 0, 24 |
| abstract_inverted_index.that | 14 |
| abstract_inverted_index.will | 29 |
| abstract_inverted_index.with | 18 |
| abstract_inverted_index.alone. | 21 |
| abstract_inverted_index.cannot | 15 |
| abstract_inverted_index.inform | 10 |
| abstract_inverted_index.advance | 31 |
| abstract_inverted_index.further | 30 |
| abstract_inverted_index.probing | 4 |
| abstract_inverted_index.behavior | 13 |
| abstract_inverted_index.clinical | 35 |
| abstract_inverted_index.research | 33 |
| abstract_inverted_index.analysis, | 26 |
| abstract_inverted_index.intensity | 19 |
| abstract_inverted_index.molecular | 5 |
| abstract_inverted_index.biological | 32 |
| abstract_inverted_index.elucidated | 17 |
| abstract_inverted_index.Development | 22 |
| abstract_inverted_index.fluorophore | 12 |
| abstract_inverted_index.advantageous | 2 |
| abstract_inverted_index.applications | 28 |
| abstract_inverted_index.assessments. | 36 |
| abstract_inverted_index.environments | 6 |
| abstract_inverted_index.fluorophores | 8 |
| abstract_inverted_index.measurements | 20 |
| abstract_inverted_index.technologies, | 25 |
| cited_by_percentile_year.max | 100 |
| cited_by_percentile_year.min | 89 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 5 |
| sustainable_development_goals[0].id | https://metadata.un.org/sdg/7 |
| sustainable_development_goals[0].score | 0.7599999904632568 |
| sustainable_development_goals[0].display_name | Affordable and clean energy |
| citation_normalized_percentile.value | 0.99955883 |
| citation_normalized_percentile.is_in_top_1_percent | True |
| citation_normalized_percentile.is_in_top_10_percent | True |