FRET‐Based Detection of Enzymatic Reaction of Botulinum on Microfluidic Device Article Swipe

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Young Min Bae , Seung Oh Jin , Insung Kim , Ki-Young Shin ·

YOU? · · 2015 · Open Access · · DOI: https://doi.org/10.1155/2015/126598

A microfluidic device was implemented to detect the enzymatic reaction of botulinum toxin A (BTA) using Förster resonance energy transfer (FRET). The microfluidic device comprised a main channel having two loading zones, a reaction chamber and a side channel perpendicular to the main channel. The reaction chamber defined by weir in the main channel was packed with microbeads. The movement of the peptide substrate and the BTA in the microfluidic device was controlled by electrophoresis, and the enzymatic reaction of the BTA was detected through the changes of the fluorescence intensity in the reaction chamber. As a result, it was observed that the enzymatic reaction was affected by the electric voltage applied for the movement of the BTA and the peptide and improved by packing the microbeads in the reaction chamber. The microfluidic device provides the tool to investigate the proteolysis of the substrate by the BTA.

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Microfluidics Materials science Förster resonance energy transfer Substrate (aquarium) Analytical Chemistry (journal) Fluorescence Nanotechnology Chromatography Chemistry Optics Geology Oceanography Physics

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Type: article
Language: en
Landing Page: https://doi.org/10.1155/2015/126598
PDF: https://downloads.hindawi.com/journals/jnm/2015/126598.pdf
OA Status: hybrid
Cited By: 2
References: 18
Related Works: 10
OpenAlex ID: https://openalex.org/W1735112997

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DOI: https://doi.org/10.1155/2015/126598

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Title: FRET‐Based Detection of Enzymatic Reaction of Botulinum on Microfluidic Device

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Language: en

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Publication year: 2015

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Publication date: 2015-01-01

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Authors: Young Min Bae, Seung Oh Jin, Insung Kim, Ki-Young Shin

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Landing page: https://doi.org/10.1155/2015/126598

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Concepts: Microfluidics, Materials science, Förster resonance energy transfer, Substrate (aquarium), Analytical Chemistry (journal), Fluorescence, Nanotechnology, Chromatography, Chemistry, Optics, Geology, Oceanography, Physics

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Cited by: 2

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abstract_inverted_index.botulinum	11
abstract_inverted_index.comprised	24
abstract_inverted_index.enzymatic	8, 77, 103
abstract_inverted_index.intensity	90
abstract_inverted_index.resonance	17
abstract_inverted_index.substrate	63, 143
abstract_inverted_index.controlled	72
abstract_inverted_index.microbeads	126
abstract_inverted_index.implemented	4
abstract_inverted_index.investigate	138
abstract_inverted_index.microbeads.	57
abstract_inverted_index.proteolysis	140
abstract_inverted_index.fluorescence	89
abstract_inverted_index.microfluidic	1, 22, 69, 132
abstract_inverted_index.perpendicular	39
abstract_inverted_index.electrophoresis,	74
cited_by_percentile_year.max	94
cited_by_percentile_year.min	90
countries_distinct_count	1
institutions_distinct_count	4
sustainable_development_goals[0].id	https://metadata.un.org/sdg/7
sustainable_development_goals[0].score	0.8299999833106995
sustainable_development_goals[0].display_name	Affordable and clean energy
citation_normalized_percentile.value	0.57308012
citation_normalized_percentile.is_in_top_1_percent	False
citation_normalized_percentile.is_in_top_10_percent	False