bioRxiv (Cold Spring Harbor Laboratory)
Fully Automated and Scalable Pipeline for Macaque Brain Registration
July 2025 • Junjie Zhuo, Z. Zhang, Chi Xiao, M. Z. Wang, Shmushkovich Bi, Liang Shi, Qingming Luo, Hui Gong, Zhiming Shen, Xiaoquan Yang, Pengcheng Li
With the rapid development of various whole-brain ex vivo imaging technologies, there is a growing need to develop cross-modal 3D image registration methods to integrate multimodal imaging datasets. While comprehensive cross-modality registration tools such as D-LMBmap and mBrainAligner have been successfully implemented for mouse brains, macaque whole-brain registration presents unique challenges. These include more pronounced non-uniform deformations in larger ex vivo specimens, greater modality-specific contras…