Gene structure and cDNA sequence of 2-Cys peroxiredoxin in the harmful algal bloom species Chattonella marina and its gene transcription under different light intensities Article Swipe

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Koki Mukai , Ayano Teramoto , Xuchun Qiu , Yohei Shimasaki , Yoko Kato‐Unoki , Jae Man Lee , Naohiro Mizoguchi , Mst Ruhina Margia Khanam , Hina Satone , Tsuneyuki Tatsuke , Takahiro Kusakabe , Yuji Oshima · YOU? · · 2017 · Open Access · · DOI: https://doi.org/10.6084/m9.figshare.5281300

We investigated the gene structure and predicted amino acid sequence of the antioxidant enzyme 2-Cys peroxiredoxin (2-Cys Prx) in the raphidophyte Chattonella marina, which is a harmful algal bloom (HAB) species. The open reading frame of 2-Cys Prx was 585 bp long and encoded a protein consisting of 195 amino acids. The putative amino acid sequence contained two cysteine residues located at the 49th and 170th amino acid positions from the N-terminal methionine residue. The sequence also possessed 2-Cys Prx characteristic motifs, F (FFYPLDFTFVCPTEI) and EVCP. The position of the 2-Cys Prx gene relative to several others (ycf59 – 2-CysPrx – rpl35 – rpl20) was the same as that found in the chloroplast genome in the raphidophyte Heterosigma akashiwo. Upstream of the 2-Cys Prx gene, possible TATA and GGA motifs recognized by nuclear-encoded plastid RNA polymerase (NEP), and a possible -10 box and -35 box recognized by plastid-encoded plastid RNA polymerase (PEP) were observed. We measured the transcript levels of 2-Cys Prx in C. marina cells grown under three different light intensities (0, 100, 1000 µmol photons m^–2 s^–1, 14-h light/8-h dark photoperiod) by quantitative PCR. The 2-Cys Prx transcript level in cells grown under the highest light intensity on day 3 was threefold that on day 0 but two lower light intensities resulted in relatively stable transcription levels. The 2-Cys Prx transcript level was significantly positively related to the H₂O₂ concentration per cell and the H₂O₂ scavenging activity per cell. These results suggest that C. marina 2-Cys Prx functions in the chloroplast and its transcription could be regulated by both NEP and PEP. Moreover, the 2-Cys Prx transcript level might increase to remove excessive H₂O₂ produced under strong light conditions in order to maintain cell proliferation activity.

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dataset

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en

Landing Page

https://doi.org/10.6084/m9.figshare.5281300

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gold

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10

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https://openalex.org/W4394127126

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https://openalex.org/W4394127126

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DOI

https://doi.org/10.6084/m9.figshare.5281300

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Title

Gene structure and cDNA sequence of 2-Cys peroxiredoxin in the harmful algal bloom species Chattonella marina and its gene transcription under different light intensities

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dataset

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en

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2017

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2017-01-01

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Koki Mukai, Ayano Teramoto, Xuchun Qiu, Yohei Shimasaki, Yoko Kato‐Unoki, Jae Man Lee, Naohiro Mizoguchi, Mst Ruhina Margia Khanam, Hina Satone, Tsuneyuki Tatsuke, Takahiro Kusakabe, Yuji Oshima

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https://doi.org/10.6084/m9.figshare.5281300

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Yes

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gold

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https://doi.org/10.6084/m9.figshare.5281300

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Gene, Biology, Genetics, Complementary DNA, Transcription (linguistics), Bloom, Algal bloom, Sequence (biology), Ecology, Phytoplankton, Nutrient, Linguistics, Philosophy

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0

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10

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