High throughput method of 16S rRNA gene sequencing library preparation for plant root microbial community profiling Article Swipe
Kie Kumaishi
,
Erika Usui
,
Kenta Suzuki
,
Shungo Kobori
,
Takumi Sato
,
Yusuke Toda
,
Hideki Takanashi
,
Satoshi Shinozaki
,
Munehiro Noda
,
Akiko Takakura
,
Kayoko Matsumoto
,
Yuji Yamasaki
,
Hisashi Tsujimoto
,
Hiroyoshi Iwata
,
Yasunori Ichihashi
·
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1038/s41598-022-23943-x
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1038/s41598-022-23943-x
Microbiota are a major component of agroecosystems. Root microbiota, which inhabit the inside and surface of plant roots, play a significant role in plant growth and health. As next-generation sequencing technology allows the capture of microbial profiles without culturing the microbes, profiling of plant microbiota has become a staple tool in plant science and agriculture. Here, we have increased sample handling efficiency in a two-step PCR amplification protocol for 16S rRNA gene sequencing of plant root microbiota, improving DNA extraction using AMPure XP magnetic beads and PCR purification using exonuclease. These modifications reduce sample handling and capture microbial diversity comparable to that obtained by the manual method. We found a buffer with AMPure XP magnetic beads enabled efficient extraction of microbial DNA directly from plant roots. We also demonstrated that purification using exonuclease before the second PCR step enabled the capture of higher degrees of microbial diversity, thus allowing for the detection of minor bacteria compared with the purification using magnetic beads in this step. In addition, our method generated comparable microbiome profile data in plant roots and soils to that of using common commercially available DNA extraction kits, such as DNeasy PowerSoil Pro Kit and FastDNA SPIN Kit for Soil. Our method offers a simple and high-throughput option for maintaining the quality of plant root microbial community profiling.
Related Topics
Concepts
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1038/s41598-022-23943-x
- https://www.nature.com/articles/s41598-022-23943-x.pdf
- OA Status
- gold
- Cited By
- 14
- References
- 58
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4309181911
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W4309181911Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1038/s41598-022-23943-xDigital Object Identifier
- Title
-
High throughput method of 16S rRNA gene sequencing library preparation for plant root microbial community profilingWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2022Year of publication
- Publication date
-
2022-11-11Full publication date if available
- Authors
-
Kie Kumaishi, Erika Usui, Kenta Suzuki, Shungo Kobori, Takumi Sato, Yusuke Toda, Hideki Takanashi, Satoshi Shinozaki, Munehiro Noda, Akiko Takakura, Kayoko Matsumoto, Yuji Yamasaki, Hisashi Tsujimoto, Hiroyoshi Iwata, Yasunori IchihashiList of authors in order
- Landing page
-
https://doi.org/10.1038/s41598-022-23943-xPublisher landing page
- PDF URL
-
https://www.nature.com/articles/s41598-022-23943-x.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://www.nature.com/articles/s41598-022-23943-x.pdfDirect OA link when available
- Concepts
-
DNA extraction, Biology, Metagenomics, 16S ribosomal RNA, Microbiome, Polymerase chain reaction, Microbial population biology, DNA sequencing, Bacteria, Computational biology, DNA, Gene, GeneticsTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
14Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 7, 2024: 2, 2023: 5Per-year citation counts (last 5 years)
- References (count)
-
58Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W4309181911 |
|---|---|
| doi | https://doi.org/10.1038/s41598-022-23943-x |
| ids.doi | https://doi.org/10.1038/s41598-022-23943-x |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/36369356 |
| ids.openalex | https://openalex.org/W4309181911 |
| fwci | 2.48781883 |
| mesh[0].qualifier_ui | Q000235 |
| mesh[0].descriptor_ui | D012336 |
| mesh[0].is_major_topic | False |
| mesh[0].qualifier_name | genetics |
| mesh[0].descriptor_name | RNA, Ribosomal, 16S |
| mesh[1].qualifier_ui | Q000235 |
| mesh[1].descriptor_ui | D004269 |
| mesh[1].is_major_topic | False |
| mesh[1].qualifier_name | genetics |
| mesh[1].descriptor_name | DNA, Bacterial |
| mesh[2].qualifier_ui | |
| mesh[2].descriptor_ui | D020459 |
| mesh[2].is_major_topic | False |
| mesh[2].qualifier_name | |
| mesh[2].descriptor_name | Genes, rRNA |
| mesh[3].qualifier_ui | Q000235 |
| mesh[3].descriptor_ui | D064307 |
| mesh[3].is_major_topic | True |
| mesh[3].qualifier_name | genetics |
| mesh[3].descriptor_name | Microbiota |
| mesh[4].qualifier_ui | Q000379 |
| mesh[4].descriptor_ui | D059014 |
| mesh[4].is_major_topic | False |
| mesh[4].qualifier_name | methods |
| mesh[4].descriptor_name | High-Throughput Nucleotide Sequencing |
| mesh[5].qualifier_ui | |
| mesh[5].descriptor_ui | D012987 |
| mesh[5].is_major_topic | False |
| mesh[5].qualifier_name | |
| mesh[5].descriptor_name | Soil |
| mesh[6].qualifier_ui | |
| mesh[6].descriptor_ui | D004247 |
| mesh[6].is_major_topic | False |
| mesh[6].qualifier_name | |
| mesh[6].descriptor_name | DNA |
| mesh[7].qualifier_ui | |
| mesh[7].descriptor_ui | D018517 |
| mesh[7].is_major_topic | False |
| mesh[7].qualifier_name | |
| mesh[7].descriptor_name | Plant Roots |
| mesh[8].qualifier_ui | Q000235 |
| mesh[8].descriptor_ui | D005092 |
| mesh[8].is_major_topic | False |
| mesh[8].qualifier_name | genetics |
| mesh[8].descriptor_name | Exonucleases |
| mesh[9].qualifier_ui | Q000235 |
| mesh[9].descriptor_ui | D012336 |
| mesh[9].is_major_topic | False |
| mesh[9].qualifier_name | genetics |
| mesh[9].descriptor_name | RNA, Ribosomal, 16S |
| mesh[10].qualifier_ui | Q000235 |
| mesh[10].descriptor_ui | D004269 |
| mesh[10].is_major_topic | False |
| mesh[10].qualifier_name | genetics |
| mesh[10].descriptor_name | DNA, Bacterial |
| mesh[11].qualifier_ui | |
| mesh[11].descriptor_ui | D020459 |
| mesh[11].is_major_topic | False |
| mesh[11].qualifier_name | |
| mesh[11].descriptor_name | Genes, rRNA |
| mesh[12].qualifier_ui | Q000235 |
| mesh[12].descriptor_ui | D064307 |
| mesh[12].is_major_topic | True |
| mesh[12].qualifier_name | genetics |
| mesh[12].descriptor_name | Microbiota |
| mesh[13].qualifier_ui | Q000379 |
| mesh[13].descriptor_ui | D059014 |
| mesh[13].is_major_topic | False |
| mesh[13].qualifier_name | methods |
| mesh[13].descriptor_name | High-Throughput Nucleotide Sequencing |
| mesh[14].qualifier_ui | |
| mesh[14].descriptor_ui | D012987 |
| mesh[14].is_major_topic | False |
| mesh[14].qualifier_name | |
| mesh[14].descriptor_name | Soil |
| mesh[15].qualifier_ui | |
| mesh[15].descriptor_ui | D004247 |
| mesh[15].is_major_topic | False |
| mesh[15].qualifier_name | |
| mesh[15].descriptor_name | DNA |
| mesh[16].qualifier_ui | |
| mesh[16].descriptor_ui | D018517 |
| mesh[16].is_major_topic | False |
| mesh[16].qualifier_name | |
| mesh[16].descriptor_name | Plant Roots |
| mesh[17].qualifier_ui | Q000235 |
| mesh[17].descriptor_ui | D005092 |
| mesh[17].is_major_topic | False |
| mesh[17].qualifier_name | genetics |
| mesh[17].descriptor_name | Exonucleases |
| type | article |
| title | High throughput method of 16S rRNA gene sequencing library preparation for plant root microbial community profiling |
| biblio.issue | 1 |
| biblio.volume | 12 |
| biblio.last_page | 19289 |
| biblio.first_page | 19289 |
| topics[0].id | https://openalex.org/T10076 |
| topics[0].field.id | https://openalex.org/fields/11 |
| topics[0].field.display_name | Agricultural and Biological Sciences |
| topics[0].score | 0.9990000128746033 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1110 |
| topics[0].subfield.display_name | Plant Science |
| topics[0].display_name | Plant-Microbe Interactions and Immunity |
| topics[1].id | https://openalex.org/T11771 |
| topics[1].field.id | https://openalex.org/fields/11 |
| topics[1].field.display_name | Agricultural and Biological Sciences |
| topics[1].score | 0.9986000061035156 |
| topics[1].domain.id | https://openalex.org/domains/1 |
| topics[1].domain.display_name | Life Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/1110 |
| topics[1].subfield.display_name | Plant Science |
| topics[1].display_name | Plant Pathogens and Resistance |
| topics[2].id | https://openalex.org/T11470 |
| topics[2].field.id | https://openalex.org/fields/11 |
| topics[2].field.display_name | Agricultural and Biological Sciences |
| topics[2].score | 0.9973000288009644 |
| topics[2].domain.id | https://openalex.org/domains/1 |
| topics[2].domain.display_name | Life Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/1110 |
| topics[2].subfield.display_name | Plant Science |
| topics[2].display_name | Legume Nitrogen Fixing Symbiosis |
| is_xpac | False |
| apc_list.value | 1890 |
| apc_list.currency | EUR |
| apc_list.value_usd | 2190 |
| apc_paid.value | 1890 |
| apc_paid.currency | EUR |
| apc_paid.value_usd | 2190 |
| concepts[0].id | https://openalex.org/C78063203 |
| concepts[0].level | 4 |
| concepts[0].score | 0.7728658318519592 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q3062596 |
| concepts[0].display_name | DNA extraction |
| concepts[1].id | https://openalex.org/C86803240 |
| concepts[1].level | 0 |
| concepts[1].score | 0.582066535949707 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[1].display_name | Biology |
| concepts[2].id | https://openalex.org/C15151743 |
| concepts[2].level | 3 |
| concepts[2].score | 0.5757781267166138 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q903778 |
| concepts[2].display_name | Metagenomics |
| concepts[3].id | https://openalex.org/C42062724 |
| concepts[3].level | 3 |
| concepts[3].score | 0.5054888725280762 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q1209205 |
| concepts[3].display_name | 16S ribosomal RNA |
| concepts[4].id | https://openalex.org/C143121216 |
| concepts[4].level | 2 |
| concepts[4].score | 0.47358399629592896 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q1330402 |
| concepts[4].display_name | Microbiome |
| concepts[5].id | https://openalex.org/C49105822 |
| concepts[5].level | 3 |
| concepts[5].score | 0.45802128314971924 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q176996 |
| concepts[5].display_name | Polymerase chain reaction |
| concepts[6].id | https://openalex.org/C81407943 |
| concepts[6].level | 3 |
| concepts[6].score | 0.4452647566795349 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q17149178 |
| concepts[6].display_name | Microbial population biology |
| concepts[7].id | https://openalex.org/C51679486 |
| concepts[7].level | 3 |
| concepts[7].score | 0.43033367395401 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q380546 |
| concepts[7].display_name | DNA sequencing |
| concepts[8].id | https://openalex.org/C523546767 |
| concepts[8].level | 2 |
| concepts[8].score | 0.4191349744796753 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q10876 |
| concepts[8].display_name | Bacteria |
| concepts[9].id | https://openalex.org/C70721500 |
| concepts[9].level | 1 |
| concepts[9].score | 0.3878069519996643 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q177005 |
| concepts[9].display_name | Computational biology |
| concepts[10].id | https://openalex.org/C552990157 |
| concepts[10].level | 2 |
| concepts[10].score | 0.28779420256614685 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q7430 |
| concepts[10].display_name | DNA |
| concepts[11].id | https://openalex.org/C104317684 |
| concepts[11].level | 2 |
| concepts[11].score | 0.2523421049118042 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q7187 |
| concepts[11].display_name | Gene |
| concepts[12].id | https://openalex.org/C54355233 |
| concepts[12].level | 1 |
| concepts[12].score | 0.14526674151420593 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q7162 |
| concepts[12].display_name | Genetics |
| keywords[0].id | https://openalex.org/keywords/dna-extraction |
| keywords[0].score | 0.7728658318519592 |
| keywords[0].display_name | DNA extraction |
| keywords[1].id | https://openalex.org/keywords/biology |
| keywords[1].score | 0.582066535949707 |
| keywords[1].display_name | Biology |
| keywords[2].id | https://openalex.org/keywords/metagenomics |
| keywords[2].score | 0.5757781267166138 |
| keywords[2].display_name | Metagenomics |
| keywords[3].id | https://openalex.org/keywords/16s-ribosomal-rna |
| keywords[3].score | 0.5054888725280762 |
| keywords[3].display_name | 16S ribosomal RNA |
| keywords[4].id | https://openalex.org/keywords/microbiome |
| keywords[4].score | 0.47358399629592896 |
| keywords[4].display_name | Microbiome |
| keywords[5].id | https://openalex.org/keywords/polymerase-chain-reaction |
| keywords[5].score | 0.45802128314971924 |
| keywords[5].display_name | Polymerase chain reaction |
| keywords[6].id | https://openalex.org/keywords/microbial-population-biology |
| keywords[6].score | 0.4452647566795349 |
| keywords[6].display_name | Microbial population biology |
| keywords[7].id | https://openalex.org/keywords/dna-sequencing |
| keywords[7].score | 0.43033367395401 |
| keywords[7].display_name | DNA sequencing |
| keywords[8].id | https://openalex.org/keywords/bacteria |
| keywords[8].score | 0.4191349744796753 |
| keywords[8].display_name | Bacteria |
| keywords[9].id | https://openalex.org/keywords/computational-biology |
| keywords[9].score | 0.3878069519996643 |
| keywords[9].display_name | Computational biology |
| keywords[10].id | https://openalex.org/keywords/dna |
| keywords[10].score | 0.28779420256614685 |
| keywords[10].display_name | DNA |
| keywords[11].id | https://openalex.org/keywords/gene |
| keywords[11].score | 0.2523421049118042 |
| keywords[11].display_name | Gene |
| keywords[12].id | https://openalex.org/keywords/genetics |
| keywords[12].score | 0.14526674151420593 |
| keywords[12].display_name | Genetics |
| language | en |
| locations[0].id | doi:10.1038/s41598-022-23943-x |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S196734849 |
| locations[0].source.issn | 2045-2322 |
| locations[0].source.type | journal |
| locations[0].source.is_oa | True |
| locations[0].source.issn_l | 2045-2322 |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | True |
| locations[0].source.display_name | Scientific Reports |
| locations[0].source.host_organization | https://openalex.org/P4310319908 |
| locations[0].source.host_organization_name | Nature Portfolio |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310319908 |
| locations[0].license | cc-by |
| locations[0].pdf_url | https://www.nature.com/articles/s41598-022-23943-x.pdf |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | https://openalex.org/licenses/cc-by |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | Scientific Reports |
| locations[0].landing_page_url | https://doi.org/10.1038/s41598-022-23943-x |
| locations[1].id | pmid:36369356 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | Scientific reports |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/36369356 |
| locations[2].id | pmh:oai:pubmedcentral.nih.gov:9652414 |
| locations[2].is_oa | True |
| locations[2].source.id | https://openalex.org/S2764455111 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | PubMed Central |
| locations[2].source.host_organization | https://openalex.org/I1299303238 |
| locations[2].source.host_organization_name | National Institutes of Health |
| locations[2].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[2].license | other-oa |
| locations[2].pdf_url | |
| locations[2].version | submittedVersion |
| locations[2].raw_type | Text |
| locations[2].license_id | https://openalex.org/licenses/other-oa |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | Sci Rep |
| locations[2].landing_page_url | https://www.ncbi.nlm.nih.gov/pmc/articles/9652414 |
| locations[3].id | pmh:oai:doaj.org/article:b0915ae2c61d47959dc562b54fb115de |
| locations[3].is_oa | True |
| locations[3].source.id | https://openalex.org/S4306401280 |
| locations[3].source.issn | |
| locations[3].source.type | repository |
| locations[3].source.is_oa | False |
| locations[3].source.issn_l | |
| locations[3].source.is_core | False |
| locations[3].source.is_in_doaj | False |
| locations[3].source.display_name | DOAJ (DOAJ: Directory of Open Access Journals) |
| locations[3].source.host_organization | |
| locations[3].source.host_organization_name | |
| locations[3].source.host_organization_lineage | |
| locations[3].license | cc-by-sa |
| locations[3].pdf_url | |
| locations[3].version | submittedVersion |
| locations[3].raw_type | article |
| locations[3].license_id | https://openalex.org/licenses/cc-by-sa |
| locations[3].is_accepted | False |
| locations[3].is_published | False |
| locations[3].raw_source_name | Scientific Reports, Vol 12, Iss 1, Pp 1-10 (2022) |
| locations[3].landing_page_url | https://doaj.org/article/b0915ae2c61d47959dc562b54fb115de |
| indexed_in | crossref, doaj, pubmed |
| authorships[0].author.id | https://openalex.org/A5003955951 |
| authorships[0].author.orcid | |
| authorships[0].author.display_name | Kie Kumaishi |
| authorships[0].countries | JP |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I4210096362 |
| authorships[0].affiliations[0].raw_affiliation_string | RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan |
| authorships[0].institutions[0].id | https://openalex.org/I4210096362 |
| authorships[0].institutions[0].ror | https://ror.org/00s05em53 |
| authorships[0].institutions[0].type | facility |
| authorships[0].institutions[0].lineage | https://openalex.org/I4210096362, https://openalex.org/I4210110652 |
| authorships[0].institutions[0].country_code | JP |
| authorships[0].institutions[0].display_name | RIKEN BioResource Research Center |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Kie Kumaishi |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan |
| authorships[1].author.id | https://openalex.org/A5061655472 |
| authorships[1].author.orcid | |
| authorships[1].author.display_name | Erika Usui |
| authorships[1].countries | JP |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I4210096362 |
| authorships[1].affiliations[0].raw_affiliation_string | RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan |
| authorships[1].institutions[0].id | https://openalex.org/I4210096362 |
| authorships[1].institutions[0].ror | https://ror.org/00s05em53 |
| authorships[1].institutions[0].type | facility |
| authorships[1].institutions[0].lineage | https://openalex.org/I4210096362, https://openalex.org/I4210110652 |
| authorships[1].institutions[0].country_code | JP |
| authorships[1].institutions[0].display_name | RIKEN BioResource Research Center |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Erika Usui |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan |
| authorships[2].author.id | https://openalex.org/A5101434891 |
| authorships[2].author.orcid | https://orcid.org/0000-0002-0639-2303 |
| authorships[2].author.display_name | Kenta Suzuki |
| authorships[2].countries | JP |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I4210096362 |
| authorships[2].affiliations[0].raw_affiliation_string | RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan |
| authorships[2].institutions[0].id | https://openalex.org/I4210096362 |
| authorships[2].institutions[0].ror | https://ror.org/00s05em53 |
| authorships[2].institutions[0].type | facility |
| authorships[2].institutions[0].lineage | https://openalex.org/I4210096362, https://openalex.org/I4210110652 |
| authorships[2].institutions[0].country_code | JP |
| authorships[2].institutions[0].display_name | RIKEN BioResource Research Center |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Kenta Suzuki |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan |
| authorships[3].author.id | https://openalex.org/A5014133803 |
| authorships[3].author.orcid | https://orcid.org/0000-0003-4995-5847 |
| authorships[3].author.display_name | Shungo Kobori |
| authorships[3].countries | JP |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I4210096362 |
| authorships[3].affiliations[0].raw_affiliation_string | RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan |
| authorships[3].institutions[0].id | https://openalex.org/I4210096362 |
| authorships[3].institutions[0].ror | https://ror.org/00s05em53 |
| authorships[3].institutions[0].type | facility |
| authorships[3].institutions[0].lineage | https://openalex.org/I4210096362, https://openalex.org/I4210110652 |
| authorships[3].institutions[0].country_code | JP |
| authorships[3].institutions[0].display_name | RIKEN BioResource Research Center |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Shungo Kobori |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan |
| authorships[4].author.id | https://openalex.org/A5082458553 |
| authorships[4].author.orcid | https://orcid.org/0000-0003-4277-0583 |
| authorships[4].author.display_name | Takumi Sato |
| authorships[4].countries | JP |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I4210096362 |
| authorships[4].affiliations[0].raw_affiliation_string | RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan |
| authorships[4].institutions[0].id | https://openalex.org/I4210096362 |
| authorships[4].institutions[0].ror | https://ror.org/00s05em53 |
| authorships[4].institutions[0].type | facility |
| authorships[4].institutions[0].lineage | https://openalex.org/I4210096362, https://openalex.org/I4210110652 |
| authorships[4].institutions[0].country_code | JP |
| authorships[4].institutions[0].display_name | RIKEN BioResource Research Center |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Takumi Sato |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan |
| authorships[5].author.id | https://openalex.org/A5032186893 |
| authorships[5].author.orcid | https://orcid.org/0000-0002-9462-6412 |
| authorships[5].author.display_name | Yusuke Toda |
| authorships[5].countries | JP |
| authorships[5].affiliations[0].institution_ids | https://openalex.org/I74801974 |
| authorships[5].affiliations[0].raw_affiliation_string | Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan |
| authorships[5].institutions[0].id | https://openalex.org/I74801974 |
| authorships[5].institutions[0].ror | https://ror.org/057zh3y96 |
| authorships[5].institutions[0].type | education |
| authorships[5].institutions[0].lineage | https://openalex.org/I74801974 |
| authorships[5].institutions[0].country_code | JP |
| authorships[5].institutions[0].display_name | The University of Tokyo |
| authorships[5].author_position | middle |
| authorships[5].raw_author_name | Yusuke Toda |
| authorships[5].is_corresponding | False |
| authorships[5].raw_affiliation_strings | Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan |
| authorships[6].author.id | https://openalex.org/A5053467366 |
| authorships[6].author.orcid | https://orcid.org/0000-0003-2251-8508 |
| authorships[6].author.display_name | Hideki Takanashi |
| authorships[6].countries | JP |
| authorships[6].affiliations[0].institution_ids | https://openalex.org/I74801974 |
| authorships[6].affiliations[0].raw_affiliation_string | Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan |
| authorships[6].institutions[0].id | https://openalex.org/I74801974 |
| authorships[6].institutions[0].ror | https://ror.org/057zh3y96 |
| authorships[6].institutions[0].type | education |
| authorships[6].institutions[0].lineage | https://openalex.org/I74801974 |
| authorships[6].institutions[0].country_code | JP |
| authorships[6].institutions[0].display_name | The University of Tokyo |
| authorships[6].author_position | middle |
| authorships[6].raw_author_name | Hideki Takanashi |
| authorships[6].is_corresponding | False |
| authorships[6].raw_affiliation_strings | Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan |
| authorships[7].author.id | https://openalex.org/A5044522993 |
| authorships[7].author.orcid | |
| authorships[7].author.display_name | Satoshi Shinozaki |
| authorships[7].affiliations[0].raw_affiliation_string | MAYEKAWA Research Institute Co., LTD, Koto-Ku, Tokyo, 135-8482, Japan |
| authorships[7].author_position | middle |
| authorships[7].raw_author_name | Satoshi Shinozaki |
| authorships[7].is_corresponding | False |
| authorships[7].raw_affiliation_strings | MAYEKAWA Research Institute Co., LTD, Koto-Ku, Tokyo, 135-8482, Japan |
| authorships[8].author.id | https://openalex.org/A5070193221 |
| authorships[8].author.orcid | |
| authorships[8].author.display_name | Munehiro Noda |
| authorships[8].affiliations[0].raw_affiliation_string | MAYEKAWA Research Institute Co., LTD, Koto-Ku, Tokyo, 135-8482, Japan |
| authorships[8].author_position | middle |
| authorships[8].raw_author_name | Munehiro Noda |
| authorships[8].is_corresponding | False |
| authorships[8].raw_affiliation_strings | MAYEKAWA Research Institute Co., LTD, Koto-Ku, Tokyo, 135-8482, Japan |
| authorships[9].author.id | https://openalex.org/A5037879867 |
| authorships[9].author.orcid | |
| authorships[9].author.display_name | Akiko Takakura |
| authorships[9].affiliations[0].raw_affiliation_string | MAYEKAWA Research Institute Co., LTD, Koto-Ku, Tokyo, 135-8482, Japan |
| authorships[9].author_position | middle |
| authorships[9].raw_author_name | Akiko Takakura |
| authorships[9].is_corresponding | False |
| authorships[9].raw_affiliation_strings | MAYEKAWA Research Institute Co., LTD, Koto-Ku, Tokyo, 135-8482, Japan |
| authorships[10].author.id | https://openalex.org/A5052184713 |
| authorships[10].author.orcid | |
| authorships[10].author.display_name | Kayoko Matsumoto |
| authorships[10].affiliations[0].raw_affiliation_string | MAYEKAWA Research Institute Co., LTD, Koto-Ku, Tokyo, 135-8482, Japan |
| authorships[10].author_position | middle |
| authorships[10].raw_author_name | Kayoko Matsumoto |
| authorships[10].is_corresponding | False |
| authorships[10].raw_affiliation_strings | MAYEKAWA Research Institute Co., LTD, Koto-Ku, Tokyo, 135-8482, Japan |
| authorships[11].author.id | https://openalex.org/A5067913388 |
| authorships[11].author.orcid | https://orcid.org/0000-0001-8943-3256 |
| authorships[11].author.display_name | Yuji Yamasaki |
| authorships[11].countries | JP |
| authorships[11].affiliations[0].institution_ids | https://openalex.org/I4588055 |
| authorships[11].affiliations[0].raw_affiliation_string | Arid Land Research Center, Tottori University, Tottori, 680-0001, Japan |
| authorships[11].institutions[0].id | https://openalex.org/I4588055 |
| authorships[11].institutions[0].ror | https://ror.org/024yc3q36 |
| authorships[11].institutions[0].type | education |
| authorships[11].institutions[0].lineage | https://openalex.org/I4588055 |
| authorships[11].institutions[0].country_code | JP |
| authorships[11].institutions[0].display_name | Tottori University |
| authorships[11].author_position | middle |
| authorships[11].raw_author_name | Yuji Yamasaki |
| authorships[11].is_corresponding | False |
| authorships[11].raw_affiliation_strings | Arid Land Research Center, Tottori University, Tottori, 680-0001, Japan |
| authorships[12].author.id | https://openalex.org/A5068549081 |
| authorships[12].author.orcid | https://orcid.org/0000-0003-0203-0759 |
| authorships[12].author.display_name | Hisashi Tsujimoto |
| authorships[12].countries | JP |
| authorships[12].affiliations[0].institution_ids | https://openalex.org/I4588055 |
| authorships[12].affiliations[0].raw_affiliation_string | Arid Land Research Center, Tottori University, Tottori, 680-0001, Japan |
| authorships[12].institutions[0].id | https://openalex.org/I4588055 |
| authorships[12].institutions[0].ror | https://ror.org/024yc3q36 |
| authorships[12].institutions[0].type | education |
| authorships[12].institutions[0].lineage | https://openalex.org/I4588055 |
| authorships[12].institutions[0].country_code | JP |
| authorships[12].institutions[0].display_name | Tottori University |
| authorships[12].author_position | middle |
| authorships[12].raw_author_name | Hisashi Tsujimoto |
| authorships[12].is_corresponding | False |
| authorships[12].raw_affiliation_strings | Arid Land Research Center, Tottori University, Tottori, 680-0001, Japan |
| authorships[13].author.id | https://openalex.org/A5084168371 |
| authorships[13].author.orcid | https://orcid.org/0000-0002-6747-7036 |
| authorships[13].author.display_name | Hiroyoshi Iwata |
| authorships[13].countries | JP |
| authorships[13].affiliations[0].institution_ids | https://openalex.org/I74801974 |
| authorships[13].affiliations[0].raw_affiliation_string | Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan |
| authorships[13].institutions[0].id | https://openalex.org/I74801974 |
| authorships[13].institutions[0].ror | https://ror.org/057zh3y96 |
| authorships[13].institutions[0].type | education |
| authorships[13].institutions[0].lineage | https://openalex.org/I74801974 |
| authorships[13].institutions[0].country_code | JP |
| authorships[13].institutions[0].display_name | The University of Tokyo |
| authorships[13].author_position | middle |
| authorships[13].raw_author_name | Hiroyoshi Iwata |
| authorships[13].is_corresponding | False |
| authorships[13].raw_affiliation_strings | Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan |
| authorships[14].author.id | https://openalex.org/A5002040196 |
| authorships[14].author.orcid | https://orcid.org/0000-0003-1935-4397 |
| authorships[14].author.display_name | Yasunori Ichihashi |
| authorships[14].countries | JP |
| authorships[14].affiliations[0].institution_ids | https://openalex.org/I4210096362 |
| authorships[14].affiliations[0].raw_affiliation_string | RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan |
| authorships[14].institutions[0].id | https://openalex.org/I4210096362 |
| authorships[14].institutions[0].ror | https://ror.org/00s05em53 |
| authorships[14].institutions[0].type | facility |
| authorships[14].institutions[0].lineage | https://openalex.org/I4210096362, https://openalex.org/I4210110652 |
| authorships[14].institutions[0].country_code | JP |
| authorships[14].institutions[0].display_name | RIKEN BioResource Research Center |
| authorships[14].author_position | last |
| authorships[14].raw_author_name | Yasunori Ichihashi |
| authorships[14].is_corresponding | False |
| authorships[14].raw_affiliation_strings | RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan |
| has_content.pdf | True |
| has_content.grobid_xml | True |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://www.nature.com/articles/s41598-022-23943-x.pdf |
| open_access.oa_status | gold |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | High throughput method of 16S rRNA gene sequencing library preparation for plant root microbial community profiling |
| has_fulltext | True |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T10076 |
| primary_topic.field.id | https://openalex.org/fields/11 |
| primary_topic.field.display_name | Agricultural and Biological Sciences |
| primary_topic.score | 0.9990000128746033 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1110 |
| primary_topic.subfield.display_name | Plant Science |
| primary_topic.display_name | Plant-Microbe Interactions and Immunity |
| related_works | https://openalex.org/W638577851, https://openalex.org/W2968354375, https://openalex.org/W2359440920, https://openalex.org/W2889550857, https://openalex.org/W3013372948, https://openalex.org/W3135997498, https://openalex.org/W4242417330, https://openalex.org/W3198631238, https://openalex.org/W4388282505, https://openalex.org/W3139084279 |
| cited_by_count | 14 |
| counts_by_year[0].year | 2025 |
| counts_by_year[0].cited_by_count | 7 |
| counts_by_year[1].year | 2024 |
| counts_by_year[1].cited_by_count | 2 |
| counts_by_year[2].year | 2023 |
| counts_by_year[2].cited_by_count | 5 |
| locations_count | 4 |
| best_oa_location.id | doi:10.1038/s41598-022-23943-x |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S196734849 |
| best_oa_location.source.issn | 2045-2322 |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | True |
| best_oa_location.source.issn_l | 2045-2322 |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | True |
| best_oa_location.source.display_name | Scientific Reports |
| best_oa_location.source.host_organization | https://openalex.org/P4310319908 |
| best_oa_location.source.host_organization_name | Nature Portfolio |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310319908 |
| best_oa_location.license | cc-by |
| best_oa_location.pdf_url | https://www.nature.com/articles/s41598-022-23943-x.pdf |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | Scientific Reports |
| best_oa_location.landing_page_url | https://doi.org/10.1038/s41598-022-23943-x |
| primary_location.id | doi:10.1038/s41598-022-23943-x |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S196734849 |
| primary_location.source.issn | 2045-2322 |
| primary_location.source.type | journal |
| primary_location.source.is_oa | True |
| primary_location.source.issn_l | 2045-2322 |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | True |
| primary_location.source.display_name | Scientific Reports |
| primary_location.source.host_organization | https://openalex.org/P4310319908 |
| primary_location.source.host_organization_name | Nature Portfolio |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310319908 |
| primary_location.license | cc-by |
| primary_location.pdf_url | https://www.nature.com/articles/s41598-022-23943-x.pdf |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | https://openalex.org/licenses/cc-by |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | Scientific Reports |
| primary_location.landing_page_url | https://doi.org/10.1038/s41598-022-23943-x |
| publication_date | 2022-11-11 |
| publication_year | 2022 |
| referenced_works | https://openalex.org/W2145151319, https://openalex.org/W2804467469, https://openalex.org/W2923759160, https://openalex.org/W2972477080, https://openalex.org/W2158787765, https://openalex.org/W2004002570, https://openalex.org/W2031050749, https://openalex.org/W2766358903, https://openalex.org/W2052504406, https://openalex.org/W2054600557, https://openalex.org/W2774168073, https://openalex.org/W2187467595, https://openalex.org/W2801691667, https://openalex.org/W3033466752, https://openalex.org/W2641965899, https://openalex.org/W3155831839, https://openalex.org/W3101623433, https://openalex.org/W2974338359, https://openalex.org/W2281227836, https://openalex.org/W2031550784, https://openalex.org/W1973831267, https://openalex.org/W2810396271, https://openalex.org/W2596895947, https://openalex.org/W2464547619, https://openalex.org/W3019834965, https://openalex.org/W2943667167, https://openalex.org/W2765998181, https://openalex.org/W2506843120, https://openalex.org/W3048718241, https://openalex.org/W1941909431, https://openalex.org/W2093527685, https://openalex.org/W2917104427, https://openalex.org/W2701219859, https://openalex.org/W2884364248, https://openalex.org/W1489969169, https://openalex.org/W2080283023, https://openalex.org/W2795668999, https://openalex.org/W2072970694, https://openalex.org/W2401404581, https://openalex.org/W2034285706, https://openalex.org/W2103296919, https://openalex.org/W2111647009, https://openalex.org/W2133856765, https://openalex.org/W2582347286, https://openalex.org/W2170024099, https://openalex.org/W2151117351, https://openalex.org/W2128743534, https://openalex.org/W2422094108, https://openalex.org/W2784214434, https://openalex.org/W2741611442, https://openalex.org/W2738854120, https://openalex.org/W2952837153, https://openalex.org/W2568184332, https://openalex.org/W2149838163, https://openalex.org/W1503417636, https://openalex.org/W2132618796, https://openalex.org/W2884455870, https://openalex.org/W2802886693 |
| referenced_works_count | 58 |
| abstract_inverted_index.a | 3, 20, 48, 64, 110, 205 |
| abstract_inverted_index.As | 28 |
| abstract_inverted_index.In | 166 |
| abstract_inverted_index.We | 108, 127 |
| abstract_inverted_index.XP | 83, 114 |
| abstract_inverted_index.as | 191 |
| abstract_inverted_index.by | 104 |
| abstract_inverted_index.in | 23, 51, 63, 163, 175 |
| abstract_inverted_index.of | 6, 16, 35, 43, 74, 120, 142, 145, 153, 182, 214 |
| abstract_inverted_index.to | 101, 180 |
| abstract_inverted_index.we | 57 |
| abstract_inverted_index.16S | 70 |
| abstract_inverted_index.DNA | 79, 122, 187 |
| abstract_inverted_index.Kit | 195, 199 |
| abstract_inverted_index.Our | 202 |
| abstract_inverted_index.PCR | 66, 87, 137 |
| abstract_inverted_index.Pro | 194 |
| abstract_inverted_index.and | 14, 26, 54, 86, 96, 178, 196, 207 |
| abstract_inverted_index.are | 2 |
| abstract_inverted_index.for | 69, 150, 200, 210 |
| abstract_inverted_index.has | 46 |
| abstract_inverted_index.our | 168 |
| abstract_inverted_index.the | 12, 33, 40, 105, 135, 140, 151, 158, 212 |
| abstract_inverted_index.Root | 8 |
| abstract_inverted_index.SPIN | 198 |
| abstract_inverted_index.also | 128 |
| abstract_inverted_index.data | 174 |
| abstract_inverted_index.from | 124 |
| abstract_inverted_index.gene | 72 |
| abstract_inverted_index.have | 58 |
| abstract_inverted_index.play | 19 |
| abstract_inverted_index.rRNA | 71 |
| abstract_inverted_index.role | 22 |
| abstract_inverted_index.root | 76, 216 |
| abstract_inverted_index.step | 138 |
| abstract_inverted_index.such | 190 |
| abstract_inverted_index.that | 102, 130, 181 |
| abstract_inverted_index.this | 164 |
| abstract_inverted_index.thus | 148 |
| abstract_inverted_index.tool | 50 |
| abstract_inverted_index.with | 112, 157 |
| abstract_inverted_index.Here, | 56 |
| abstract_inverted_index.Soil. | 201 |
| abstract_inverted_index.These | 91 |
| abstract_inverted_index.beads | 85, 116, 162 |
| abstract_inverted_index.found | 109 |
| abstract_inverted_index.kits, | 189 |
| abstract_inverted_index.major | 4 |
| abstract_inverted_index.minor | 154 |
| abstract_inverted_index.plant | 17, 24, 44, 52, 75, 125, 176, 215 |
| abstract_inverted_index.roots | 177 |
| abstract_inverted_index.soils | 179 |
| abstract_inverted_index.step. | 165 |
| abstract_inverted_index.using | 81, 89, 132, 160, 183 |
| abstract_inverted_index.which | 10 |
| abstract_inverted_index.AMPure | 82, 113 |
| abstract_inverted_index.DNeasy | 192 |
| abstract_inverted_index.allows | 32 |
| abstract_inverted_index.become | 47 |
| abstract_inverted_index.before | 134 |
| abstract_inverted_index.buffer | 111 |
| abstract_inverted_index.common | 184 |
| abstract_inverted_index.growth | 25 |
| abstract_inverted_index.higher | 143 |
| abstract_inverted_index.inside | 13 |
| abstract_inverted_index.manual | 106 |
| abstract_inverted_index.method | 169, 203 |
| abstract_inverted_index.offers | 204 |
| abstract_inverted_index.option | 209 |
| abstract_inverted_index.reduce | 93 |
| abstract_inverted_index.roots, | 18 |
| abstract_inverted_index.roots. | 126 |
| abstract_inverted_index.sample | 60, 94 |
| abstract_inverted_index.second | 136 |
| abstract_inverted_index.simple | 206 |
| abstract_inverted_index.staple | 49 |
| abstract_inverted_index.FastDNA | 197 |
| abstract_inverted_index.capture | 34, 97, 141 |
| abstract_inverted_index.degrees | 144 |
| abstract_inverted_index.enabled | 117, 139 |
| abstract_inverted_index.health. | 27 |
| abstract_inverted_index.inhabit | 11 |
| abstract_inverted_index.method. | 107 |
| abstract_inverted_index.profile | 173 |
| abstract_inverted_index.quality | 213 |
| abstract_inverted_index.science | 53 |
| abstract_inverted_index.surface | 15 |
| abstract_inverted_index.without | 38 |
| abstract_inverted_index.Abstract | 0 |
| abstract_inverted_index.allowing | 149 |
| abstract_inverted_index.bacteria | 155 |
| abstract_inverted_index.compared | 156 |
| abstract_inverted_index.directly | 123 |
| abstract_inverted_index.handling | 61, 95 |
| abstract_inverted_index.magnetic | 84, 115, 161 |
| abstract_inverted_index.obtained | 103 |
| abstract_inverted_index.profiles | 37 |
| abstract_inverted_index.protocol | 68 |
| abstract_inverted_index.two-step | 65 |
| abstract_inverted_index.PowerSoil | 193 |
| abstract_inverted_index.addition, | 167 |
| abstract_inverted_index.available | 186 |
| abstract_inverted_index.community | 218 |
| abstract_inverted_index.component | 5 |
| abstract_inverted_index.culturing | 39 |
| abstract_inverted_index.detection | 152 |
| abstract_inverted_index.diversity | 99 |
| abstract_inverted_index.efficient | 118 |
| abstract_inverted_index.generated | 170 |
| abstract_inverted_index.improving | 78 |
| abstract_inverted_index.increased | 59 |
| abstract_inverted_index.microbes, | 41 |
| abstract_inverted_index.microbial | 36, 98, 121, 146, 217 |
| abstract_inverted_index.profiling | 42 |
| abstract_inverted_index.Microbiota | 1 |
| abstract_inverted_index.comparable | 100, 171 |
| abstract_inverted_index.diversity, | 147 |
| abstract_inverted_index.efficiency | 62 |
| abstract_inverted_index.extraction | 80, 119, 188 |
| abstract_inverted_index.microbiome | 172 |
| abstract_inverted_index.microbiota | 45 |
| abstract_inverted_index.profiling. | 219 |
| abstract_inverted_index.sequencing | 30, 73 |
| abstract_inverted_index.technology | 31 |
| abstract_inverted_index.exonuclease | 133 |
| abstract_inverted_index.maintaining | 211 |
| abstract_inverted_index.microbiota, | 9, 77 |
| abstract_inverted_index.significant | 21 |
| abstract_inverted_index.agriculture. | 55 |
| abstract_inverted_index.commercially | 185 |
| abstract_inverted_index.demonstrated | 129 |
| abstract_inverted_index.exonuclease. | 90 |
| abstract_inverted_index.purification | 88, 131, 159 |
| abstract_inverted_index.amplification | 67 |
| abstract_inverted_index.modifications | 92 |
| abstract_inverted_index.agroecosystems. | 7 |
| abstract_inverted_index.high-throughput | 208 |
| abstract_inverted_index.next-generation | 29 |
| cited_by_percentile_year.max | 99 |
| cited_by_percentile_year.min | 94 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 15 |
| sustainable_development_goals[0].id | https://metadata.un.org/sdg/2 |
| sustainable_development_goals[0].score | 0.7099999785423279 |
| sustainable_development_goals[0].display_name | Zero hunger |
| citation_normalized_percentile.value | 0.90886499 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |