Quantitative ptychographic bio-imaging in the water window Article Swipe

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Water window Ptychography Optics Synchrotron radiation Materials science Phase-contrast imaging Image resolution Radiation Diffraction Dynamic range Resolution (logic) Biological imaging Refractive index Detector Coherent diffraction imaging Physics Phase retrieval Phase contrast microscopy Artificial intelligence Fourier transform Fluorescence Quantum mechanics Computer science

M. Franklin Rose , Tobias Senkbeil , Andreas Robert von Gundlach , Susan Stuhr , Christoph Rumancev , Dmitry Dzhigaev , Ilya S. Besedin , Petr Skopintsev , Lars Loetgering , Jens Viefhaus , Axel Rosenhahn , Ivan A. Vartanyants ·

YOU? · · 2018 · Open Access · · DOI: https://doi.org/10.1364/oe.26.001237 · OA: W2783649616

Coherent X-ray ptychography is a tool for highly dose efficient lensless nano-imaging of biological samples. We have used partially coherent soft X-ray synchrotron radiation to obtain a quantitative image of a laterally extended, dried, and unstained fibroblast cell by ptychography. We used data with and without a beam stop that allowed us to measure coherent diffraction with a high-dynamic range of 1.7·10<sup>6</sup>. As a quantitative result, we obtained the refractive index values for two regions of the cell with respect to a reference area. Due to the photon energy in the water window we obtained an extremely high contrast of 53% at 71 nm half-period resolution. The dose applied in our experiment was 9.5·10<sup>4</sup> Gy and is well below the radiation damage threshold. The concept for dynamic range improvement for low dynamic range detectors with a beam stop opens the path for high resolution nano-imaging of a variety of samples including cryo-preserved, hydrated and unstained biological cells.