IgG from players with elevated anti-S100B levels recognize glial and neuronal epitopes. Article Swipe
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· 2015
· Open Access
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· DOI: https://doi.org/10.1371/journal.pone.0056805.g006
· OA: W226213769
<p>Serum samples obtained from selected players (highest and lowest auto-S100B antibody titers, see # and * in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056805#pone-0056805-g004" target="_blank">Figure 4</a>) where used as “primary” antibody for recognition of brain epitopes. A control porcine brain was used (see text for details). <b>A</b>) No signal was observed when serum was omitted or when brain slices were incubated with <50 kDa serum fractions devoid of IgG. <b>B</b>) Low levels of brain immune reactivity was observed when using the serum sample from the player with lowest auto-S100B antibody levels. <b>C–D</b>) A more pronounced signal was observed when using the serum from the most “auto-immune” sample. Both glial and neuronal staining were stained by these IgGs, supporting the hypothesis that auto-immunity is not limited to the glial protein S100B but rather extends to other neuronal epitopes.</p>
