Image scanning microscopy with a doughnut beam: signal strength and integrated intensity Article Swipe
Colin J. R. Sheppard
,
Marco Castello
,
Giorgio Tortarolo
,
Alessandro Zunino
,
Eli Slenders
,
Paolo Bianchini
,
Giuseppe Vicidomini
,
Alberto Diaspro
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1364/josaa.495984
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1364/josaa.495984
We discuss the effects of image scanning microscopy using doughnut beam illumination on the properties of signal strength and integrated intensity. Doughnut beam illumination can give better optical sectioning and background rejection than Airy disk illumination. The outer pixels of a detector array give a signal from defocused regions, so digital processing of these (e.g., by simple subtraction) can further improve optical sectioning and background rejection from a single in-focus scan.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1364/josaa.495984
- OA Status
- green
- References
- 36
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4384282589
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4384282589Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1364/josaa.495984Digital Object Identifier
- Title
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Image scanning microscopy with a doughnut beam: signal strength and integrated intensityWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2023Year of publication
- Publication date
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2023-07-14Full publication date if available
- Authors
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Colin J. R. Sheppard, Marco Castello, Giorgio Tortarolo, Alessandro Zunino, Eli Slenders, Paolo Bianchini, Giuseppe Vicidomini, Alberto DiasproList of authors in order
- Landing page
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https://doi.org/10.1364/josaa.495984Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
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greenOpen access status per OpenAlex
- OA URL
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https://infoscience.epfl.ch/record/304873Direct OA link when available
- Concepts
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Optics, Optical sectioning, SIGNAL (programming language), Pixel, Beam (structure), Microscopy, Detector, Signal beam, Intensity (physics), Materials science, Focus (optics), Physics, Computer science, Programming languageTop concepts (fields/topics) attached by OpenAlex
- Cited by
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0Total citation count in OpenAlex
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36Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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