Imaging mechanical properties of sub-micron ECM in live zebrafish using Brillouin microscopy Article Swipe
Carlo Bevilacqua
,
Héctor Sánchez-Iranzo
,
Dmitry Richter
,
Alba Diz-Muñoz
,
Robert Prevedel
·
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.1364/boe.10.001420
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.1364/boe.10.001420
In this work, we quantify the mechanical properties of the extra-cellular matrix (ECM) in live zebrafish using Brillouin microscopy. Optimization of the imaging conditions and parameters, combined with careful spectral analysis, allows us to resolve the thin ECM and distinguish its Brillouin frequency shift, a proxy for mechanical properties, from the surrounding tissue. High-resolution mechanical mapping further enables the direct measurement of the thickness of the ECM label-free and in-vivo. We find the ECM to be ~500 nm thick, and in very good agreement with electron microscopy quantification. Our results open the door for future studies that aim to investigate the role of ECM mechanics for zebrafish morphogenesis and axis elongation.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1364/boe.10.001420
- OA Status
- gold
- Cited By
- 81
- References
- 42
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2949219767
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W2949219767Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1364/boe.10.001420Digital Object Identifier
- Title
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Imaging mechanical properties of sub-micron ECM in live zebrafish using Brillouin microscopyWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2019Year of publication
- Publication date
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2019-02-25Full publication date if available
- Authors
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Carlo Bevilacqua, Héctor Sánchez-Iranzo, Dmitry Richter, Alba Diz-Muñoz, Robert PrevedelList of authors in order
- Landing page
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https://doi.org/10.1364/boe.10.001420Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://doi.org/10.1364/boe.10.001420Direct OA link when available
- Concepts
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Brillouin zone, Microscopy, Zebrafish, Materials science, Live cell imaging, Morphogenesis, Optics, Nanotechnology, Biomedical engineering, Chemistry, Physics, Gene, Medicine, Biochemistry, CellTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
81Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 12, 2024: 15, 2023: 9, 2022: 13, 2021: 13Per-year citation counts (last 5 years)
- References (count)
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42Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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