In-Depth Genomic Characterization of a Meropenem-nonsusceptible Pseudomonas otitidis Strain Contaminating Chicken Carcass Article Swipe
Tatiana Regina Vieira
,
Gustavo Enck Sambrano
,
Núbia Michelle Vieira da Silva
,
Priscylla Carvalho Vasconcelos
,
Esther Ferraza Cavinatto de Oliveira
,
Celso José Bruno de Oliveira
,
Samuel Paulo Cibulski
,
Marisa Ribeiro de Itapema Cardoso
·
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.22456/1679-9216.103176
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.22456/1679-9216.103176
Background: The indiscriminate use of antibiotics in food-animal production has a major impact on public health, particularly in terms of contributing to the emergence and dissemination of antimicrobial resistant bacteria in the food-animal production chain. Although Pseudomonas species are recognized as important spoilage organisms in foodstuff, they are also known as opportunistic pathogens associated with hospital-acquired infections. Furthermore, Pseudomonas can play a role as potential reservoirs of antimicrobial resistance genes, which may be horizontally transferred to other bacteria. Considering that cephalosporins (3rd and higher generations) and carbapenems are critically important beta-lactam antimicrobials in human medicine, this study reports the occurrence and genomic characterization of a meropenem-nonsusceptible Pseudomonas otitidis strain recovered from a chicken carcass in Brazil.Materials, Methods & Results: During the years 2018-2019, 72 frozen chicken carcasses were purchased on the retail market from different regions in Brazil. Aliquots from individual carcass rinses were screened for Extended Spectrum Beta-lactamase (ESBL)-producing bacteria in MacConkey agar supplemented with 1mg.L-1 cefotaxime. Phenotypically resistant isolates were further tested for resistance to other antimicrobials and confirmed as ESBL-producers by means of disk-diffusion method using Müller-Hinton agar. Only one meropenen-nonsusceptible isolate was detected and submitted to whole genome sequencing (WGS) in Illumina Miseq. The strain was identified as Pseudomonas otitidis by local alignment of the 16S rRNA sequence using BLASTn and confirmed by Average Nucleotide Identity (ANI) analysis using JspeciesWS database. Genes encoding for antimicrobial resistance were detected by means of Resfinder and Comprehensive Antibiotic Resistance Database (CARD) databases. The phenotypic non-susceptibility to meropenen was attributed to the gene blaPOM-1. A total of 192 different genes encoding for quorum sensing system, antiphagocytosis, iron uptake, efflux pump, endotoxin and toxin, adherence, and secretion systems were detected by means of Virulence Factor Database (VFDB). Pseudomonas otitidis-pan genome was built using Roary-rapid large-scale prokaryote pan genome analysis using the present strain (K_25) and other two P. otitidis genomes (PAM-1, DSM 17224) publicly available at the NCBI. The core genome analysis of the two human strains resulted in similar percentages.Discussion: Carbapenems are critically important drugs for human health and bacterial strains resistant to these antimicrobials pose a public health problem. The blaPOM-1 gene harbored by the Pseudomonas otitidis K_25 strain encodes a metallo-beta-lactamase (MBL) conferring resistance to carbapenems. Pseudomonas otitidis was the first confirmed pathogenic Pseudomonas species expressing MBL constitutively in the absence of inducible beta-lactamase genes. Furthermore, the several virulence genes associated with the capacity of the P. otitidis K_25 to colonize, evade the immune system and cause lesions in the human host confirm this strain as a potential opportunistic pathogen contaminating foodstuff. These reinforce the need to address antimicrobial resistance in a One Health perspective, in which resistant bacteria and resistance determinants circulate among environment, animals and humans.
Related Topics
Concepts
Cefotaxime
Biology
Microbiology
Antibiotic resistance
Antimicrobial
Cephalosporin
Meropenem
Pseudomonas
MacConkey agar
Multilocus sequence typing
Pseudomonas aeruginosa
Antibiotics
Bacteria
Agar
Genotype
Gene
Genetics
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.22456/1679-9216.103176
- https://seer.ufrgs.br/ActaScientiaeVeterinariae/article/download/103176/pdf
- OA Status
- diamond
- Cited By
- 4
- References
- 25
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3040268995
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W3040268995Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.22456/1679-9216.103176Digital Object Identifier
- Title
-
In-Depth Genomic Characterization of a Meropenem-nonsusceptible Pseudomonas otitidis Strain Contaminating Chicken CarcassWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2020Year of publication
- Publication date
-
2020-01-23Full publication date if available
- Authors
-
Tatiana Regina Vieira, Gustavo Enck Sambrano, Núbia Michelle Vieira da Silva, Priscylla Carvalho Vasconcelos, Esther Ferraza Cavinatto de Oliveira, Celso José Bruno de Oliveira, Samuel Paulo Cibulski, Marisa Ribeiro de Itapema CardosoList of authors in order
- Landing page
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https://doi.org/10.22456/1679-9216.103176Publisher landing page
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https://seer.ufrgs.br/ActaScientiaeVeterinariae/article/download/103176/pdfDirect link to full text PDF
- Open access
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YesWhether a free full text is available
- OA status
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diamondOpen access status per OpenAlex
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https://seer.ufrgs.br/ActaScientiaeVeterinariae/article/download/103176/pdfDirect OA link when available
- Concepts
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Cefotaxime, Biology, Microbiology, Antibiotic resistance, Antimicrobial, Cephalosporin, Meropenem, Pseudomonas, MacConkey agar, Multilocus sequence typing, Pseudomonas aeruginosa, Antibiotics, Bacteria, Agar, Genotype, Gene, GeneticsTop concepts (fields/topics) attached by OpenAlex
- Cited by
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4Total citation count in OpenAlex
- Citations by year (recent)
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2025: 1, 2023: 2, 2022: 1Per-year citation counts (last 5 years)
- References (count)
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25Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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