In Silico and Therapeutic Efficacy of Glycyrrhizin‐Based Silver Nanoparticles Against Bacteria Related to Periodontitis Article Swipe
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· 2025
· Open Access
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· DOI: https://doi.org/10.1002/slct.202405251
· OA: W4406101477
Rising antibiotic resistance among periodontal pathogens poses a major challenge to treatment outcomes. This work investigates the possible combinatorial potential of AgNPs synthesized using glycyrrhizin, a natural biosurfactant, to combat biofilm‐forming bacteria isolated from periodontitis patients. In silico findings revealed that glycyrrhizin had a higher binding affinity score of ‐9.102 compared to doxycycline (−3.599) for the gtfB , plays an indispensable function in biofilm integrity. AgNPs were synthesized using glycyrrhizin and optimized utilizing the design of experiments, considering factors such as pH, temperature, incubation time and glycyrrhizin concentration as variables. while The resulting GL‐AgNPs were characterized by different analytical techniques such as FTIR, XRD, DLS, and SEM. The nanoparticles were non‐spherical, uniform and had an average size of 72 nm, while zeta potentials ranged from −28 mV to − 42 mV. Antimicrobial findings suggested that GL‐AgNPs exhibited significant antibacterial and anti‐biofilm activity compared to AgNPs prepared using sodium borohydride ( p < 0.001). Experimental findings suggested that GL‐AgNPs inhibited biofilm formation by attenuating Gtfase activity. Further, GL‐AgNPs show effective anti‐inflammatory activity with minimal cytotoxicity. Overall, this study highlights that the inherent antimicrobial and surfactant properties of glycyrrhizin greatly improve the antimicrobial potential of AgNPs, providing a promising approach to combating biofilm‐producing oral pathogens in periodontitis.
