Induction of DNA Single Strand Breaks and Base Damages by Ultra-High Dose Rate Irradiation Article Swipe

View

Yu-Cheng Wang , Yan Zhang , Chenyang Huang , Qibin Fu , T. Huang ·

YOU? · · 2025 · Open Access · · DOI: https://doi.org/10.20944/preprints202501.2082.v1

Background: The study of different types of DNA damage after ultra-high dose rate irradiation (UHDR) is of great significance for further understanding the mechanism of FLASH effect. Methods: pBR322 plasmid DNA was irradiated by electron FLASH beam. The content of each subtype of plasmid DNA was measured by gel electrophoresis, and the extent of DNA double strand break (DSBs) and single strand break (SSBs) under UHDR and conventional dose rate irradiation (CONV) was quantitatively compared. Further, by adding the endonuclease Nth and Fpg, the extent of base damage in UHDR and CONV group was quantitatively analyzed. In addition, the effects of different plasmid concentrations on the damage degree were also studied. Results: The induction rates of SSBs (×10−3 SSB/Gy/molecule) of UHDR and CONV were 21.7±0.4 and 25.8±0.3, respectively. When treated with Fpg and Nth enzyme, base damage induction rates (×10−3 SSB/Gy/molecule) of UHDR and CONV were 43.3±2.0 and 58.4±4.5, respectively. Additionally, it was found that plasmid concentration also affected the degree of damage, with lower SSB induction at higher plasmid concentration for both FLASH and CONV. For high-concentration plasmids, the induction rates of SSBs of UHDR was still 14% lower than that of CONV. Conclusions: UHDR has significant effect on reducing SSB and base damage compared to CONV regardless of plasmid concentration.

Related Topics

Concepts

Plasmid DNA damage DNA Molecular biology Irradiation Chemistry Base excision repair Endonuclease Biology Biophysics Biochemistry Physics Nuclear physics

Metadata

Type: preprint
Language: en
Landing Page: https://doi.org/10.20944/preprints202501.2082.v1
OA Status: green
Related Works: 10
OpenAlex ID: https://openalex.org/W4407037475

All OpenAlex metadata

Raw OpenAlex JSON

OpenAlex ID: https://openalex.org/W4407037475

Canonical identifier for this work in OpenAlex
DOI: https://doi.org/10.20944/preprints202501.2082.v1

Digital Object Identifier
Title: Induction of DNA Single Strand Breaks and Base Damages by Ultra-High Dose Rate Irradiation

Work title
Type: preprint

OpenAlex work type
Language: en

Primary language
Publication year: 2025

Year of publication
Publication date: 2025-01-28

Full publication date if available
Authors: Yu-Cheng Wang, Yan Zhang, Chenyang Huang, Qibin Fu, T. Huang

List of authors in order
Landing page: https://doi.org/10.20944/preprints202501.2082.v1

Publisher landing page
Open access: Yes

Whether a free full text is available
OA status: green

Open access status per OpenAlex
OA URL: https://doi.org/10.20944/preprints202501.2082.v1

Direct OA link when available
Concepts: Plasmid, DNA damage, DNA, Molecular biology, Irradiation, Chemistry, Base excision repair, Endonuclease, Biology, Biophysics, Biochemistry, Physics, Nuclear physics

Top concepts (fields/topics) attached by OpenAlex
Cited by: 0

Total citation count in OpenAlex
Related works (count): 10

Other works algorithmically related by OpenAlex

Full payload

id	https://openalex.org/W4407037475
doi	https://doi.org/10.20944/preprints202501.2082.v1
ids.doi	https://doi.org/10.20944/preprints202501.2082.v1
ids.openalex	https://openalex.org/W4407037475
fwci	0.0
type	preprint
title	Induction of DNA Single Strand Breaks and Base Damages by Ultra-High Dose Rate Irradiation
biblio.issue
biblio.volume
biblio.last_page
biblio.first_page
topics[0].id	https://openalex.org/T10123
topics[0].field.id	https://openalex.org/fields/13
topics[0].field.display_name	Biochemistry, Genetics and Molecular Biology
topics[0].score	0.9977999925613403
topics[0].domain.id	https://openalex.org/domains/1
topics[0].domain.display_name	Life Sciences
topics[0].subfield.id	https://openalex.org/subfields/1312
topics[0].subfield.display_name	Molecular Biology
topics[0].display_name	DNA Repair Mechanisms
topics[1].id	https://openalex.org/T12828
topics[1].field.id	https://openalex.org/fields/11
topics[1].field.display_name	Agricultural and Biological Sciences
topics[1].score	0.9879000186920166
topics[1].domain.id	https://openalex.org/domains/1
topics[1].domain.display_name	Life Sciences
topics[1].subfield.id	https://openalex.org/subfields/1110
topics[1].subfield.display_name	Plant Science
topics[1].display_name	Plant Genetic and Mutation Studies
topics[2].id	https://openalex.org/T10433
topics[2].field.id	https://openalex.org/fields/13
topics[2].field.display_name	Biochemistry, Genetics and Molecular Biology
topics[2].score	0.9876000285148621
topics[2].domain.id	https://openalex.org/domains/1
topics[2].domain.display_name	Life Sciences
topics[2].subfield.id	https://openalex.org/subfields/1306
topics[2].subfield.display_name	Cancer Research
topics[2].display_name	Carcinogens and Genotoxicity Assessment
is_xpac	False
apc_list
apc_paid
concepts[0].id	https://openalex.org/C22744801
concepts[0].level	3
concepts[0].score	0.8181250095367432
concepts[0].wikidata	https://www.wikidata.org/wiki/Q172778
concepts[0].display_name	Plasmid
concepts[1].id	https://openalex.org/C143425029
concepts[1].level	3
concepts[1].score	0.6620815992355347
concepts[1].wikidata	https://www.wikidata.org/wiki/Q5205747
concepts[1].display_name	DNA damage
concepts[2].id	https://openalex.org/C552990157
concepts[2].level	2
concepts[2].score	0.6170376539230347
concepts[2].wikidata	https://www.wikidata.org/wiki/Q7430
concepts[2].display_name	DNA
concepts[3].id	https://openalex.org/C153911025
concepts[3].level	1
concepts[3].score	0.6090487241744995
concepts[3].wikidata	https://www.wikidata.org/wiki/Q7202
concepts[3].display_name	Molecular biology
concepts[4].id	https://openalex.org/C111337013
concepts[4].level	2
concepts[4].score	0.5844643115997314
concepts[4].wikidata	https://www.wikidata.org/wiki/Q2737837
concepts[4].display_name	Irradiation
concepts[5].id	https://openalex.org/C185592680
concepts[5].level	0
concepts[5].score	0.4594477415084839
concepts[5].wikidata	https://www.wikidata.org/wiki/Q2329
concepts[5].display_name	Chemistry
concepts[6].id	https://openalex.org/C187206112
concepts[6].level	4
concepts[6].score	0.4354096055030823
concepts[6].wikidata	https://www.wikidata.org/wiki/Q3113091
concepts[6].display_name	Base excision repair
concepts[7].id	https://openalex.org/C2777028655
concepts[7].level	3
concepts[7].score	0.4241769313812256
concepts[7].wikidata	https://www.wikidata.org/wiki/Q415601
concepts[7].display_name	Endonuclease
concepts[8].id	https://openalex.org/C86803240
concepts[8].level	0
concepts[8].score	0.3882283568382263
concepts[8].wikidata	https://www.wikidata.org/wiki/Q420
concepts[8].display_name	Biology
concepts[9].id	https://openalex.org/C12554922
concepts[9].level	1
concepts[9].score	0.37401628494262695
concepts[9].wikidata	https://www.wikidata.org/wiki/Q7100
concepts[9].display_name	Biophysics
concepts[10].id	https://openalex.org/C55493867
concepts[10].level	1
concepts[10].score	0.28280454874038696
concepts[10].wikidata	https://www.wikidata.org/wiki/Q7094
concepts[10].display_name	Biochemistry
concepts[11].id	https://openalex.org/C121332964
concepts[11].level	0
concepts[11].score	0.10664239525794983
concepts[11].wikidata	https://www.wikidata.org/wiki/Q413
concepts[11].display_name	Physics
concepts[12].id	https://openalex.org/C185544564
concepts[12].level	1
concepts[12].score	0.0
concepts[12].wikidata	https://www.wikidata.org/wiki/Q81197
concepts[12].display_name	Nuclear physics
keywords[0].id	https://openalex.org/keywords/plasmid
keywords[0].score	0.8181250095367432
keywords[0].display_name	Plasmid
keywords[1].id	https://openalex.org/keywords/dna-damage
keywords[1].score	0.6620815992355347
keywords[1].display_name	DNA damage
keywords[2].id	https://openalex.org/keywords/dna
keywords[2].score	0.6170376539230347
keywords[2].display_name	DNA
keywords[3].id	https://openalex.org/keywords/molecular-biology
keywords[3].score	0.6090487241744995
keywords[3].display_name	Molecular biology
keywords[4].id	https://openalex.org/keywords/irradiation
keywords[4].score	0.5844643115997314
keywords[4].display_name	Irradiation
keywords[5].id	https://openalex.org/keywords/chemistry
keywords[5].score	0.4594477415084839
keywords[5].display_name	Chemistry
keywords[6].id	https://openalex.org/keywords/base-excision-repair
keywords[6].score	0.4354096055030823
keywords[6].display_name	Base excision repair
keywords[7].id	https://openalex.org/keywords/endonuclease
keywords[7].score	0.4241769313812256
keywords[7].display_name	Endonuclease
keywords[8].id	https://openalex.org/keywords/biology
keywords[8].score	0.3882283568382263
keywords[8].display_name	Biology
keywords[9].id	https://openalex.org/keywords/biophysics
keywords[9].score	0.37401628494262695
keywords[9].display_name	Biophysics
keywords[10].id	https://openalex.org/keywords/biochemistry
keywords[10].score	0.28280454874038696
keywords[10].display_name	Biochemistry
keywords[11].id	https://openalex.org/keywords/physics
keywords[11].score	0.10664239525794983
keywords[11].display_name	Physics
language	en
locations[0].id	doi:10.20944/preprints202501.2082.v1
locations[0].is_oa	True
locations[0].source.id	https://openalex.org/S6309402219
locations[0].source.issn
locations[0].source.type	repository
locations[0].source.is_oa	True
locations[0].source.issn_l
locations[0].source.is_core	False
locations[0].source.is_in_doaj	False
locations[0].source.display_name	Preprints.org
locations[0].source.host_organization
locations[0].source.host_organization_name
locations[0].source.host_organization_lineage	https://openalex.org/P4310310987
locations[0].license	cc-by
locations[0].pdf_url
locations[0].version	acceptedVersion
locations[0].raw_type	posted-content
locations[0].license_id	https://openalex.org/licenses/cc-by
locations[0].is_accepted	True
locations[0].is_published	False
locations[0].raw_source_name
locations[0].landing_page_url	https://doi.org/10.20944/preprints202501.2082.v1
indexed_in	crossref
authorships[0].author.id	https://openalex.org/A5004314911
authorships[0].author.orcid	https://orcid.org/0000-0003-4339-1665
authorships[0].author.display_name	Yu-Cheng Wang
authorships[0].author_position	first
authorships[0].raw_author_name	Yu-Cheng Wang
authorships[0].is_corresponding	False
authorships[1].author.id	https://openalex.org/A5100456179
authorships[1].author.orcid	https://orcid.org/0000-0001-9890-8380
authorships[1].author.display_name	Yan Zhang
authorships[1].author_position	middle
authorships[1].raw_author_name	Yan Zhang
authorships[1].is_corresponding	False
authorships[2].author.id	https://openalex.org/A5033759838
authorships[2].author.orcid	https://orcid.org/0000-0002-1956-6229
authorships[2].author.display_name	Chenyang Huang
authorships[2].author_position	middle
authorships[2].raw_author_name	Chen-Yang Huang
authorships[2].is_corresponding	False
authorships[3].author.id	https://openalex.org/A5048503720
authorships[3].author.orcid	https://orcid.org/0000-0002-1419-5676
authorships[3].author.display_name	Qibin Fu
authorships[3].author_position	middle
authorships[3].raw_author_name	Qi-Bin Fu
authorships[3].is_corresponding	False
authorships[4].author.id	https://openalex.org/A5061302013
authorships[4].author.orcid	https://orcid.org/0000-0002-4786-4530
authorships[4].author.display_name	T. Huang
authorships[4].author_position	last
authorships[4].raw_author_name	Tu-Chen Huang
authorships[4].is_corresponding	False
has_content.pdf	False
has_content.grobid_xml	False
is_paratext	False
open_access.is_oa	True
open_access.oa_url	https://doi.org/10.20944/preprints202501.2082.v1
open_access.oa_status	green
open_access.any_repository_has_fulltext	False
created_date	2025-10-10T00:00:00
display_name	Induction of DNA Single Strand Breaks and Base Damages by Ultra-High Dose Rate Irradiation
has_fulltext	False
is_retracted	False
updated_date	2025-11-06T03:46:38.306776
primary_topic.id	https://openalex.org/T10123
primary_topic.field.id	https://openalex.org/fields/13
primary_topic.field.display_name	Biochemistry, Genetics and Molecular Biology
primary_topic.score	0.9977999925613403
primary_topic.domain.id	https://openalex.org/domains/1
primary_topic.domain.display_name	Life Sciences
primary_topic.subfield.id	https://openalex.org/subfields/1312
primary_topic.subfield.display_name	Molecular Biology
primary_topic.display_name	DNA Repair Mechanisms
related_works	https://openalex.org/W4391250611, https://openalex.org/W2145349694, https://openalex.org/W2135553932, https://openalex.org/W2084216648, https://openalex.org/W1981453126, https://openalex.org/W2092459944, https://openalex.org/W3213598712, https://openalex.org/W2022547913, https://openalex.org/W2169804829, https://openalex.org/W2416549882
cited_by_count	0
locations_count	1
best_oa_location.id	doi:10.20944/preprints202501.2082.v1
best_oa_location.is_oa	True
best_oa_location.source.id	https://openalex.org/S6309402219
best_oa_location.source.issn
best_oa_location.source.type	repository
best_oa_location.source.is_oa	True
best_oa_location.source.issn_l
best_oa_location.source.is_core	False
best_oa_location.source.is_in_doaj	False
best_oa_location.source.display_name	Preprints.org
best_oa_location.source.host_organization
best_oa_location.source.host_organization_name
best_oa_location.source.host_organization_lineage	https://openalex.org/P4310310987
best_oa_location.license	cc-by
best_oa_location.pdf_url
best_oa_location.version	acceptedVersion
best_oa_location.raw_type	posted-content
best_oa_location.license_id	https://openalex.org/licenses/cc-by
best_oa_location.is_accepted	True
best_oa_location.is_published	False
best_oa_location.raw_source_name
best_oa_location.landing_page_url	https://doi.org/10.20944/preprints202501.2082.v1
primary_location.id	doi:10.20944/preprints202501.2082.v1
primary_location.is_oa	True
primary_location.source.id	https://openalex.org/S6309402219
primary_location.source.issn
primary_location.source.type	repository
primary_location.source.is_oa	True
primary_location.source.issn_l
primary_location.source.is_core	False
primary_location.source.is_in_doaj	False
primary_location.source.display_name	Preprints.org
primary_location.source.host_organization
primary_location.source.host_organization_name
primary_location.source.host_organization_lineage	https://openalex.org/P4310310987
primary_location.license	cc-by
primary_location.pdf_url
primary_location.version	acceptedVersion
primary_location.raw_type	posted-content
primary_location.license_id	https://openalex.org/licenses/cc-by
primary_location.is_accepted	True
primary_location.is_published	False
primary_location.raw_source_name
primary_location.landing_page_url	https://doi.org/10.20944/preprints202501.2082.v1
publication_date	2025-01-28
publication_year	2025
referenced_works_count	0
abstract_inverted_index.In	96
abstract_inverted_index.at	167
abstract_inverted_index.by	33, 47, 76
abstract_inverted_index.in	88
abstract_inverted_index.is	15
abstract_inverted_index.it	151
abstract_inverted_index.of	3, 6, 16, 24, 39, 42, 53, 85, 100, 115, 119, 141, 161, 182, 184, 192, 209
abstract_inverted_index.on	104, 199
abstract_inverted_index.to	206
abstract_inverted_index.14%	188
abstract_inverted_index.DNA	7, 30, 44, 54
abstract_inverted_index.For	176
abstract_inverted_index.Fpg	131
abstract_inverted_index.Nth	80, 133
abstract_inverted_index.SSB	165, 201
abstract_inverted_index.The	1, 37, 112
abstract_inverted_index.and	50, 59, 66, 81, 90, 121, 125, 132, 143, 147, 174, 202
abstract_inverted_index.for	19, 171
abstract_inverted_index.gel	48
abstract_inverted_index.has	196
abstract_inverted_index.the	22, 51, 78, 83, 98, 105, 159, 179
abstract_inverted_index.was	31, 45, 72, 93, 152, 186
abstract_inverted_index.CONV	91, 122, 144, 207
abstract_inverted_index.Fpg,	82
abstract_inverted_index.SSBs	116, 183
abstract_inverted_index.UHDR	65, 89, 120, 142, 185, 195
abstract_inverted_index.When	128
abstract_inverted_index.also	109, 157
abstract_inverted_index.base	86, 135, 203
abstract_inverted_index.both	172
abstract_inverted_index.dose	11, 68
abstract_inverted_index.each	40
abstract_inverted_index.rate	12, 69
abstract_inverted_index.than	190
abstract_inverted_index.that	154, 191
abstract_inverted_index.were	108, 123, 145
abstract_inverted_index.with	130, 163
abstract_inverted_index.CONV.	175, 193
abstract_inverted_index.FLASH	25, 35, 173
abstract_inverted_index.after	9
abstract_inverted_index.beam.	36
abstract_inverted_index.break	57, 62
abstract_inverted_index.found	153
abstract_inverted_index.great	17
abstract_inverted_index.group	92
abstract_inverted_index.lower	164, 189
abstract_inverted_index.rates	114, 138, 181
abstract_inverted_index.still	187
abstract_inverted_index.study	2
abstract_inverted_index.types	5
abstract_inverted_index.under	64
abstract_inverted_index.(CONV)	71
abstract_inverted_index.(DSBs)	58
abstract_inverted_index.(SSBs)	63
abstract_inverted_index.(UHDR)	14
abstract_inverted_index.adding	77
abstract_inverted_index.damage	8, 87, 106, 136, 204
abstract_inverted_index.degree	107, 160
abstract_inverted_index.double	55
abstract_inverted_index.effect	198
abstract_inverted_index.extent	52, 84
abstract_inverted_index.higher	168
abstract_inverted_index.pBR322	28
abstract_inverted_index.single	60
abstract_inverted_index.strand	56, 61
abstract_inverted_index.content	38
abstract_inverted_index.damage,	162
abstract_inverted_index.effect.	26
abstract_inverted_index.effects	99
abstract_inverted_index.enzyme,	134
abstract_inverted_index.further	20
abstract_inverted_index.plasmid	29, 43, 102, 155, 169, 210
abstract_inverted_index.subtype	41
abstract_inverted_index.treated	129
abstract_inverted_index.Further,	75
abstract_inverted_index.Methods:	27
abstract_inverted_index.Results:	111
abstract_inverted_index.affected	158
abstract_inverted_index.compared	205
abstract_inverted_index.electron	34
abstract_inverted_index.measured	46
abstract_inverted_index.reducing	200
abstract_inverted_index.studied.	110
abstract_inverted_index.addition,	97
abstract_inverted_index.analyzed.	95
abstract_inverted_index.compared.	74
abstract_inverted_index.different	4, 101
abstract_inverted_index.induction	113, 137, 166, 180
abstract_inverted_index.mechanism	23
abstract_inverted_index.plasmids,	178
abstract_inverted_index.irradiated	32
abstract_inverted_index.regardless	208
abstract_inverted_index.ultra-high	10
abstract_inverted_index.Background:	0
abstract_inverted_index.irradiation	13, 70
abstract_inverted_index.significant	197
abstract_inverted_index.Conclusions:	194
abstract_inverted_index.conventional	67
abstract_inverted_index.endonuclease	79
abstract_inverted_index.significance	18
abstract_inverted_index.Additionally,	150
abstract_inverted_index.concentration	156, 170
abstract_inverted_index.respectively.	127, 149
abstract_inverted_index.understanding	21
abstract_inverted_index.concentration.	211
abstract_inverted_index.concentrations	103
abstract_inverted_index.quantitatively	73, 94
abstract_inverted_index.SSB/Gy/molecule)	118, 140
abstract_inverted_index.electrophoresis,	49
abstract_inverted_index.high-concentration	177
abstract_inverted_index.21.7&plusmn;0.4	124
abstract_inverted_index.43.3&plusmn;2.0	146
abstract_inverted_index.25.8&plusmn;0.3,	126
abstract_inverted_index.58.4&plusmn;4.5,	148
abstract_inverted_index.(&times;10&minus;3	117, 139
cited_by_percentile_year
countries_distinct_count	0
institutions_distinct_count	5
citation_normalized_percentile.value	0.02842072
citation_normalized_percentile.is_in_top_1_percent	False
citation_normalized_percentile.is_in_top_10_percent	False