Integrating Reverse Transcription Recombinase Polymerase Amplification with CRISPR Technology for the One-Tube Assay of RNA Article Swipe
Wei Feng
,
Hanyong Peng
,
Jingyang Xu
,
Yanming Liu
,
Kanti Pabbaraju
,
Graham Tipples
,
Michael Joyce
,
Holly A. Saffran
,
D. Lorne Tyrrell
,
Shawn Babiuk
,
Hongquan Zhang
,
X. Chris Le
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1021/acs.analchem.1c03456
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1021/acs.analchem.1c03456
CRISPR-Cas systems integrated with nucleic acid amplification techniques improve both analytical specificity and sensitivity. We describe here issues and solutions for the successful integration of reverse transcription (RT), recombinase polymerase amplification (RPA), and CRISPR-Cas12a nuclease reactions into a single tube under an isothermal condition (40 °C). Specific detection of a few copies of a viral DNA sequence was achieved in less than 20 min. However, the sensitivity was orders of magnitude lower for the detection of viral RNA due to the slow initiation of RPA when the complementary DNA (cDNA) template remained hybridized to RNA. During the delay of RPA, the crRNA-Cas12a ribonucleoprotein (RNP) gradually lost its activity in the RPA solution, and nonspecific amplification reactions consumed the RPA reagents. We overcame these problems by taking advantage of the endoribonuclease function of RNase H to remove RNA from the RNA-cDNA hybrids and free the cDNA as template for the RPA reaction. As a consequence, we significantly enhanced the overall reaction rate of an integrated assay using RT-RPA and CRISPR-Cas12a for the detection of RNA. We showed successful detection of 200 or more copies of the S gene sequence of SARS-CoV-2 RNA within 5-30 min. We applied our one-tube assay to 46 upper respiratory swab samples for COVID-19 diagnosis, and the results from both fluorescence intensity measurements and end-point visualization were consistent with those of RT-qPCR analysis. The strategy and technique improve the sensitivity and speed of RT-RPA and CRISPR-Cas12a assays, potentially useful for both semi-quantitative and point-of-care analyses of RNA molecules.
Related Topics
Concepts
Recombinase Polymerase Amplification
Trans-activating crRNA
RNA
Molecular biology
Chemistry
Reverse transcriptase
Complementary DNA
CRISPR
Amplicon
Nucleic acid
DNA
RNase P
Cas9
Loop-mediated isothermal amplification
Biology
Gene
Polymerase chain reaction
Biochemistry
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1021/acs.analchem.1c03456
- https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.1c03456
- OA Status
- bronze
- Cited By
- 110
- References
- 27
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3197021219
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W3197021219Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1021/acs.analchem.1c03456Digital Object Identifier
- Title
-
Integrating Reverse Transcription Recombinase Polymerase Amplification with CRISPR Technology for the One-Tube Assay of RNAWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2021Year of publication
- Publication date
-
2021-09-10Full publication date if available
- Authors
-
Wei Feng, Hanyong Peng, Jingyang Xu, Yanming Liu, Kanti Pabbaraju, Graham Tipples, Michael Joyce, Holly A. Saffran, D. Lorne Tyrrell, Shawn Babiuk, Hongquan Zhang, X. Chris LeList of authors in order
- Landing page
-
https://doi.org/10.1021/acs.analchem.1c03456Publisher landing page
- PDF URL
-
https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.1c03456Direct link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
bronzeOpen access status per OpenAlex
- OA URL
-
https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.1c03456Direct OA link when available
- Concepts
-
Recombinase Polymerase Amplification, Trans-activating crRNA, RNA, Molecular biology, Chemistry, Reverse transcriptase, Complementary DNA, CRISPR, Amplicon, Nucleic acid, DNA, RNase P, Cas9, Loop-mediated isothermal amplification, Biology, Gene, Polymerase chain reaction, BiochemistryTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
110Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 27, 2024: 25, 2023: 28, 2022: 29, 2021: 1Per-year citation counts (last 5 years)
- References (count)
-
27Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W3197021219 |
|---|---|
| doi | https://doi.org/10.1021/acs.analchem.1c03456 |
| ids.doi | https://doi.org/10.1021/acs.analchem.1c03456 |
| ids.mag | 3197021219 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/34506127 |
| ids.openalex | https://openalex.org/W3197021219 |
| fwci | 8.09519146 |
| mesh[0].qualifier_ui | |
| mesh[0].descriptor_ui | D000086382 |
| mesh[0].is_major_topic | True |
| mesh[0].qualifier_name | |
| mesh[0].descriptor_name | COVID-19 |
| mesh[1].qualifier_ui | |
| mesh[1].descriptor_ui | D000086742 |
| mesh[1].is_major_topic | False |
| mesh[1].qualifier_name | |
| mesh[1].descriptor_name | COVID-19 Testing |
| mesh[2].qualifier_ui | |
| mesh[2].descriptor_ui | D006801 |
| mesh[2].is_major_topic | False |
| mesh[2].qualifier_name | |
| mesh[2].descriptor_name | Humans |
| mesh[3].qualifier_ui | |
| mesh[3].descriptor_ui | D021141 |
| mesh[3].is_major_topic | False |
| mesh[3].qualifier_name | |
| mesh[3].descriptor_name | Nucleic Acid Amplification Techniques |
| mesh[4].qualifier_ui | Q000235 |
| mesh[4].descriptor_ui | D012367 |
| mesh[4].is_major_topic | False |
| mesh[4].qualifier_name | genetics |
| mesh[4].descriptor_name | RNA, Viral |
| mesh[5].qualifier_ui | Q000235 |
| mesh[5].descriptor_ui | D045522 |
| mesh[5].is_major_topic | False |
| mesh[5].qualifier_name | genetics |
| mesh[5].descriptor_name | Recombinases |
| mesh[6].qualifier_ui | |
| mesh[6].descriptor_ui | D048348 |
| mesh[6].is_major_topic | True |
| mesh[6].qualifier_name | |
| mesh[6].descriptor_name | Reverse Transcription |
| mesh[7].qualifier_ui | |
| mesh[7].descriptor_ui | D000086402 |
| mesh[7].is_major_topic | False |
| mesh[7].qualifier_name | |
| mesh[7].descriptor_name | SARS-CoV-2 |
| mesh[8].qualifier_ui | |
| mesh[8].descriptor_ui | D012680 |
| mesh[8].is_major_topic | False |
| mesh[8].qualifier_name | |
| mesh[8].descriptor_name | Sensitivity and Specificity |
| mesh[9].qualifier_ui | |
| mesh[9].descriptor_ui | D013672 |
| mesh[9].is_major_topic | False |
| mesh[9].qualifier_name | |
| mesh[9].descriptor_name | Technology |
| mesh[10].qualifier_ui | |
| mesh[10].descriptor_ui | D000086382 |
| mesh[10].is_major_topic | True |
| mesh[10].qualifier_name | |
| mesh[10].descriptor_name | COVID-19 |
| mesh[11].qualifier_ui | |
| mesh[11].descriptor_ui | D000086742 |
| mesh[11].is_major_topic | False |
| mesh[11].qualifier_name | |
| mesh[11].descriptor_name | COVID-19 Testing |
| mesh[12].qualifier_ui | |
| mesh[12].descriptor_ui | D006801 |
| mesh[12].is_major_topic | False |
| mesh[12].qualifier_name | |
| mesh[12].descriptor_name | Humans |
| mesh[13].qualifier_ui | |
| mesh[13].descriptor_ui | D021141 |
| mesh[13].is_major_topic | False |
| mesh[13].qualifier_name | |
| mesh[13].descriptor_name | Nucleic Acid Amplification Techniques |
| mesh[14].qualifier_ui | Q000235 |
| mesh[14].descriptor_ui | D012367 |
| mesh[14].is_major_topic | False |
| mesh[14].qualifier_name | genetics |
| mesh[14].descriptor_name | RNA, Viral |
| mesh[15].qualifier_ui | Q000235 |
| mesh[15].descriptor_ui | D045522 |
| mesh[15].is_major_topic | False |
| mesh[15].qualifier_name | genetics |
| mesh[15].descriptor_name | Recombinases |
| mesh[16].qualifier_ui | |
| mesh[16].descriptor_ui | D048348 |
| mesh[16].is_major_topic | True |
| mesh[16].qualifier_name | |
| mesh[16].descriptor_name | Reverse Transcription |
| mesh[17].qualifier_ui | |
| mesh[17].descriptor_ui | D000086402 |
| mesh[17].is_major_topic | False |
| mesh[17].qualifier_name | |
| mesh[17].descriptor_name | SARS-CoV-2 |
| mesh[18].qualifier_ui | |
| mesh[18].descriptor_ui | D012680 |
| mesh[18].is_major_topic | False |
| mesh[18].qualifier_name | |
| mesh[18].descriptor_name | Sensitivity and Specificity |
| mesh[19].qualifier_ui | |
| mesh[19].descriptor_ui | D013672 |
| mesh[19].is_major_topic | False |
| mesh[19].qualifier_name | |
| mesh[19].descriptor_name | Technology |
| type | article |
| title | Integrating Reverse Transcription Recombinase Polymerase Amplification with CRISPR Technology for the One-Tube Assay of RNA |
| biblio.issue | 37 |
| biblio.volume | 93 |
| biblio.last_page | 12816 |
| biblio.first_page | 12808 |
| grants[0].funder | https://openalex.org/F4320319880 |
| grants[0].award_id | |
| grants[0].funder_display_name | Government of Canada |
| grants[1].funder | https://openalex.org/F4320322725 |
| grants[1].award_id | |
| grants[1].funder_display_name | China Scholarship Council |
| grants[2].funder | https://openalex.org/F4320325651 |
| grants[2].award_id | |
| grants[2].funder_display_name | Alberta Innovates |
| grants[3].funder | https://openalex.org/F4320334506 |
| grants[3].award_id | |
| grants[3].funder_display_name | Canadian Institutes of Health Research |
| grants[4].funder | https://openalex.org/F4320334593 |
| grants[4].award_id | |
| grants[4].funder_display_name | Natural Sciences and Engineering Research Council of Canada |
| grants[5].funder | https://openalex.org/F4320336499 |
| grants[5].award_id | |
| grants[5].funder_display_name | Li Ka Shing Institute of Virology, University of Alberta |
| topics[0].id | https://openalex.org/T10878 |
| topics[0].field.id | https://openalex.org/fields/13 |
| topics[0].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[0].score | 1.0 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1312 |
| topics[0].subfield.display_name | Molecular Biology |
| topics[0].display_name | CRISPR and Genetic Engineering |
| topics[1].id | https://openalex.org/T10207 |
| topics[1].field.id | https://openalex.org/fields/13 |
| topics[1].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[1].score | 0.9940999746322632 |
| topics[1].domain.id | https://openalex.org/domains/1 |
| topics[1].domain.display_name | Life Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/1312 |
| topics[1].subfield.display_name | Molecular Biology |
| topics[1].display_name | Advanced biosensing and bioanalysis techniques |
| topics[2].id | https://openalex.org/T11393 |
| topics[2].field.id | https://openalex.org/fields/22 |
| topics[2].field.display_name | Engineering |
| topics[2].score | 0.9879000186920166 |
| topics[2].domain.id | https://openalex.org/domains/3 |
| topics[2].domain.display_name | Physical Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/2204 |
| topics[2].subfield.display_name | Biomedical Engineering |
| topics[2].display_name | Biosensors and Analytical Detection |
| funders[0].id | https://openalex.org/F4320319880 |
| funders[0].ror | https://ror.org/010q4q527 |
| funders[0].display_name | Government of Canada |
| funders[1].id | https://openalex.org/F4320322725 |
| funders[1].ror | https://ror.org/04atp4p48 |
| funders[1].display_name | China Scholarship Council |
| funders[2].id | https://openalex.org/F4320325651 |
| funders[2].ror | |
| funders[2].display_name | Alberta Innovates |
| funders[3].id | https://openalex.org/F4320334506 |
| funders[3].ror | https://ror.org/01gavpb45 |
| funders[3].display_name | Canadian Institutes of Health Research |
| funders[4].id | https://openalex.org/F4320334593 |
| funders[4].ror | https://ror.org/01h531d29 |
| funders[4].display_name | Natural Sciences and Engineering Research Council of Canada |
| funders[5].id | https://openalex.org/F4320336499 |
| funders[5].ror | |
| funders[5].display_name | Li Ka Shing Institute of Virology, University of Alberta |
| is_xpac | False |
| apc_list | |
| apc_paid | |
| concepts[0].id | https://openalex.org/C139713532 |
| concepts[0].level | 4 |
| concepts[0].score | 0.9355195164680481 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q17077657 |
| concepts[0].display_name | Recombinase Polymerase Amplification |
| concepts[1].id | https://openalex.org/C84356384 |
| concepts[1].level | 5 |
| concepts[1].score | 0.8158870935440063 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q7833575 |
| concepts[1].display_name | Trans-activating crRNA |
| concepts[2].id | https://openalex.org/C67705224 |
| concepts[2].level | 3 |
| concepts[2].score | 0.6360787153244019 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q11053 |
| concepts[2].display_name | RNA |
| concepts[3].id | https://openalex.org/C153911025 |
| concepts[3].level | 1 |
| concepts[3].score | 0.6043649315834045 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q7202 |
| concepts[3].display_name | Molecular biology |
| concepts[4].id | https://openalex.org/C185592680 |
| concepts[4].level | 0 |
| concepts[4].score | 0.5908402800559998 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q2329 |
| concepts[4].display_name | Chemistry |
| concepts[5].id | https://openalex.org/C156719811 |
| concepts[5].level | 4 |
| concepts[5].score | 0.5340021848678589 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q28774 |
| concepts[5].display_name | Reverse transcriptase |
| concepts[6].id | https://openalex.org/C187882448 |
| concepts[6].level | 3 |
| concepts[6].score | 0.5115512013435364 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q283478 |
| concepts[6].display_name | Complementary DNA |
| concepts[7].id | https://openalex.org/C98108389 |
| concepts[7].level | 3 |
| concepts[7].score | 0.4836534261703491 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q412563 |
| concepts[7].display_name | CRISPR |
| concepts[8].id | https://openalex.org/C8185291 |
| concepts[8].level | 4 |
| concepts[8].score | 0.4830797016620636 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q2844457 |
| concepts[8].display_name | Amplicon |
| concepts[9].id | https://openalex.org/C24107716 |
| concepts[9].level | 2 |
| concepts[9].score | 0.4676717519760132 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q123619 |
| concepts[9].display_name | Nucleic acid |
| concepts[10].id | https://openalex.org/C552990157 |
| concepts[10].level | 2 |
| concepts[10].score | 0.4527873396873474 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q7430 |
| concepts[10].display_name | DNA |
| concepts[11].id | https://openalex.org/C10879258 |
| concepts[11].level | 4 |
| concepts[11].score | 0.42508214712142944 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q76826611 |
| concepts[11].display_name | RNase P |
| concepts[12].id | https://openalex.org/C132455925 |
| concepts[12].level | 4 |
| concepts[12].score | 0.36939528584480286 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q16965677 |
| concepts[12].display_name | Cas9 |
| concepts[13].id | https://openalex.org/C60635243 |
| concepts[13].level | 3 |
| concepts[13].score | 0.34062913060188293 |
| concepts[13].wikidata | https://www.wikidata.org/wiki/Q6675780 |
| concepts[13].display_name | Loop-mediated isothermal amplification |
| concepts[14].id | https://openalex.org/C86803240 |
| concepts[14].level | 0 |
| concepts[14].score | 0.2936623692512512 |
| concepts[14].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[14].display_name | Biology |
| concepts[15].id | https://openalex.org/C104317684 |
| concepts[15].level | 2 |
| concepts[15].score | 0.2746913433074951 |
| concepts[15].wikidata | https://www.wikidata.org/wiki/Q7187 |
| concepts[15].display_name | Gene |
| concepts[16].id | https://openalex.org/C49105822 |
| concepts[16].level | 3 |
| concepts[16].score | 0.27231982350349426 |
| concepts[16].wikidata | https://www.wikidata.org/wiki/Q176996 |
| concepts[16].display_name | Polymerase chain reaction |
| concepts[17].id | https://openalex.org/C55493867 |
| concepts[17].level | 1 |
| concepts[17].score | 0.09506157040596008 |
| concepts[17].wikidata | https://www.wikidata.org/wiki/Q7094 |
| concepts[17].display_name | Biochemistry |
| keywords[0].id | https://openalex.org/keywords/recombinase-polymerase-amplification |
| keywords[0].score | 0.9355195164680481 |
| keywords[0].display_name | Recombinase Polymerase Amplification |
| keywords[1].id | https://openalex.org/keywords/trans-activating-crrna |
| keywords[1].score | 0.8158870935440063 |
| keywords[1].display_name | Trans-activating crRNA |
| keywords[2].id | https://openalex.org/keywords/rna |
| keywords[2].score | 0.6360787153244019 |
| keywords[2].display_name | RNA |
| keywords[3].id | https://openalex.org/keywords/molecular-biology |
| keywords[3].score | 0.6043649315834045 |
| keywords[3].display_name | Molecular biology |
| keywords[4].id | https://openalex.org/keywords/chemistry |
| keywords[4].score | 0.5908402800559998 |
| keywords[4].display_name | Chemistry |
| keywords[5].id | https://openalex.org/keywords/reverse-transcriptase |
| keywords[5].score | 0.5340021848678589 |
| keywords[5].display_name | Reverse transcriptase |
| keywords[6].id | https://openalex.org/keywords/complementary-dna |
| keywords[6].score | 0.5115512013435364 |
| keywords[6].display_name | Complementary DNA |
| keywords[7].id | https://openalex.org/keywords/crispr |
| keywords[7].score | 0.4836534261703491 |
| keywords[7].display_name | CRISPR |
| keywords[8].id | https://openalex.org/keywords/amplicon |
| keywords[8].score | 0.4830797016620636 |
| keywords[8].display_name | Amplicon |
| keywords[9].id | https://openalex.org/keywords/nucleic-acid |
| keywords[9].score | 0.4676717519760132 |
| keywords[9].display_name | Nucleic acid |
| keywords[10].id | https://openalex.org/keywords/dna |
| keywords[10].score | 0.4527873396873474 |
| keywords[10].display_name | DNA |
| keywords[11].id | https://openalex.org/keywords/rnase-p |
| keywords[11].score | 0.42508214712142944 |
| keywords[11].display_name | RNase P |
| keywords[12].id | https://openalex.org/keywords/cas9 |
| keywords[12].score | 0.36939528584480286 |
| keywords[12].display_name | Cas9 |
| keywords[13].id | https://openalex.org/keywords/loop-mediated-isothermal-amplification |
| keywords[13].score | 0.34062913060188293 |
| keywords[13].display_name | Loop-mediated isothermal amplification |
| keywords[14].id | https://openalex.org/keywords/biology |
| keywords[14].score | 0.2936623692512512 |
| keywords[14].display_name | Biology |
| keywords[15].id | https://openalex.org/keywords/gene |
| keywords[15].score | 0.2746913433074951 |
| keywords[15].display_name | Gene |
| keywords[16].id | https://openalex.org/keywords/polymerase-chain-reaction |
| keywords[16].score | 0.27231982350349426 |
| keywords[16].display_name | Polymerase chain reaction |
| keywords[17].id | https://openalex.org/keywords/biochemistry |
| keywords[17].score | 0.09506157040596008 |
| keywords[17].display_name | Biochemistry |
| language | en |
| locations[0].id | doi:10.1021/acs.analchem.1c03456 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S191963794 |
| locations[0].source.issn | 0003-2700, 1520-6882 |
| locations[0].source.type | journal |
| locations[0].source.is_oa | False |
| locations[0].source.issn_l | 0003-2700 |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | False |
| locations[0].source.display_name | Analytical Chemistry |
| locations[0].source.host_organization | https://openalex.org/P4310320006 |
| locations[0].source.host_organization_name | American Chemical Society |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310320006 |
| locations[0].license | |
| locations[0].pdf_url | https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.1c03456 |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | Analytical Chemistry |
| locations[0].landing_page_url | https://doi.org/10.1021/acs.analchem.1c03456 |
| locations[1].id | pmid:34506127 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | Analytical chemistry |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/34506127 |
| indexed_in | crossref, pubmed |
| authorships[0].author.id | https://openalex.org/A5054236676 |
| authorships[0].author.orcid | https://orcid.org/0009-0001-0173-874X |
| authorships[0].author.display_name | Wei Feng |
| authorships[0].countries | CA |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I154425047 |
| authorships[0].affiliations[0].raw_affiliation_string | Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2G3, Canada |
| authorships[0].institutions[0].id | https://openalex.org/I154425047 |
| authorships[0].institutions[0].ror | https://ror.org/0160cpw27 |
| authorships[0].institutions[0].type | education |
| authorships[0].institutions[0].lineage | https://openalex.org/I154425047 |
| authorships[0].institutions[0].country_code | CA |
| authorships[0].institutions[0].display_name | University of Alberta |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Wei Feng |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2G3, Canada |
| authorships[1].author.id | https://openalex.org/A5071428253 |
| authorships[1].author.orcid | https://orcid.org/0000-0001-7079-0827 |
| authorships[1].author.display_name | Hanyong Peng |
| authorships[1].countries | CA, CN |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I154425047 |
| authorships[1].affiliations[0].raw_affiliation_string | Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2G3, Canada |
| authorships[1].affiliations[1].institution_ids | https://openalex.org/I19820366, https://openalex.org/I4210144289 |
| authorships[1].affiliations[1].raw_affiliation_string | State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China |
| authorships[1].institutions[0].id | https://openalex.org/I154425047 |
| authorships[1].institutions[0].ror | https://ror.org/0160cpw27 |
| authorships[1].institutions[0].type | education |
| authorships[1].institutions[0].lineage | https://openalex.org/I154425047 |
| authorships[1].institutions[0].country_code | CA |
| authorships[1].institutions[0].display_name | University of Alberta |
| authorships[1].institutions[1].id | https://openalex.org/I19820366 |
| authorships[1].institutions[1].ror | https://ror.org/034t30j35 |
| authorships[1].institutions[1].type | government |
| authorships[1].institutions[1].lineage | https://openalex.org/I19820366 |
| authorships[1].institutions[1].country_code | CN |
| authorships[1].institutions[1].display_name | Chinese Academy of Sciences |
| authorships[1].institutions[2].id | https://openalex.org/I4210144289 |
| authorships[1].institutions[2].ror | https://ror.org/03rpsvy57 |
| authorships[1].institutions[2].type | facility |
| authorships[1].institutions[2].lineage | https://openalex.org/I19820366, https://openalex.org/I4210144289 |
| authorships[1].institutions[2].country_code | CN |
| authorships[1].institutions[2].display_name | Research Center for Eco-Environmental Sciences |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Hanyong Peng |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2G3, Canada, State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China |
| authorships[2].author.id | https://openalex.org/A5103002314 |
| authorships[2].author.orcid | https://orcid.org/0000-0002-5561-6385 |
| authorships[2].author.display_name | Jingyang Xu |
| authorships[2].countries | CA |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I154425047 |
| authorships[2].affiliations[0].raw_affiliation_string | Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2G3, Canada |
| authorships[2].institutions[0].id | https://openalex.org/I154425047 |
| authorships[2].institutions[0].ror | https://ror.org/0160cpw27 |
| authorships[2].institutions[0].type | education |
| authorships[2].institutions[0].lineage | https://openalex.org/I154425047 |
| authorships[2].institutions[0].country_code | CA |
| authorships[2].institutions[0].display_name | University of Alberta |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Jingyang Xu |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2G3, Canada |
| authorships[3].author.id | https://openalex.org/A5100393269 |
| authorships[3].author.orcid | https://orcid.org/0000-0002-3498-367X |
| authorships[3].author.display_name | Yanming Liu |
| authorships[3].countries | CA |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I154425047 |
| authorships[3].affiliations[0].raw_affiliation_string | Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2G3, Canada |
| authorships[3].institutions[0].id | https://openalex.org/I154425047 |
| authorships[3].institutions[0].ror | https://ror.org/0160cpw27 |
| authorships[3].institutions[0].type | education |
| authorships[3].institutions[0].lineage | https://openalex.org/I154425047 |
| authorships[3].institutions[0].country_code | CA |
| authorships[3].institutions[0].display_name | University of Alberta |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Yanming Liu |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2G3, Canada |
| authorships[4].author.id | https://openalex.org/A5013201074 |
| authorships[4].author.orcid | https://orcid.org/0000-0002-7409-579X |
| authorships[4].author.display_name | Kanti Pabbaraju |
| authorships[4].countries | CA |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I4210090353 |
| authorships[4].affiliations[0].raw_affiliation_string | Provincial Laboratory for Public Health (ProvLab), Alberta Precision Laboratories, 3030 Hospital Drive, Calgary, Alberta T2N 4W4, Canada |
| authorships[4].institutions[0].id | https://openalex.org/I4210090353 |
| authorships[4].institutions[0].ror | https://ror.org/009afvb77 |
| authorships[4].institutions[0].type | facility |
| authorships[4].institutions[0].lineage | https://openalex.org/I4210090353 |
| authorships[4].institutions[0].country_code | CA |
| authorships[4].institutions[0].display_name | Provincial Laboratory of Public Health |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Kanti Pabbaraju |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | Provincial Laboratory for Public Health (ProvLab), Alberta Precision Laboratories, 3030 Hospital Drive, Calgary, Alberta T2N 4W4, Canada |
| authorships[5].author.id | https://openalex.org/A5030821767 |
| authorships[5].author.orcid | https://orcid.org/0000-0002-2116-8553 |
| authorships[5].author.display_name | Graham Tipples |
| authorships[5].countries | CA |
| authorships[5].affiliations[0].institution_ids | https://openalex.org/I2802925319, https://openalex.org/I4210090353 |
| authorships[5].affiliations[0].raw_affiliation_string | Provincial Laboratory for Public Health, Alberta Precision Laboratories, University of Alberta Hospitals, 8440-112 St, Edmonton, Alberta T6G 2J2, Canada |
| authorships[5].affiliations[1].institution_ids | https://openalex.org/I154425047 |
| authorships[5].affiliations[1].raw_affiliation_string | Li Ka Shing Institute of Virology, Department of Medical Microbiology and Immunology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2E1, Canada |
| authorships[5].institutions[0].id | https://openalex.org/I4210090353 |
| authorships[5].institutions[0].ror | https://ror.org/009afvb77 |
| authorships[5].institutions[0].type | facility |
| authorships[5].institutions[0].lineage | https://openalex.org/I4210090353 |
| authorships[5].institutions[0].country_code | CA |
| authorships[5].institutions[0].display_name | Provincial Laboratory of Public Health |
| authorships[5].institutions[1].id | https://openalex.org/I154425047 |
| authorships[5].institutions[1].ror | https://ror.org/0160cpw27 |
| authorships[5].institutions[1].type | education |
| authorships[5].institutions[1].lineage | https://openalex.org/I154425047 |
| authorships[5].institutions[1].country_code | CA |
| authorships[5].institutions[1].display_name | University of Alberta |
| authorships[5].institutions[2].id | https://openalex.org/I2802925319 |
| authorships[5].institutions[2].ror | https://ror.org/013e81n30 |
| authorships[5].institutions[2].type | healthcare |
| authorships[5].institutions[2].lineage | https://openalex.org/I126859394, https://openalex.org/I2802925319 |
| authorships[5].institutions[2].country_code | CA |
| authorships[5].institutions[2].display_name | University of Alberta Hospital |
| authorships[5].author_position | middle |
| authorships[5].raw_author_name | Graham Tipples |
| authorships[5].is_corresponding | False |
| authorships[5].raw_affiliation_strings | Li Ka Shing Institute of Virology, Department of Medical Microbiology and Immunology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2E1, Canada, Provincial Laboratory for Public Health, Alberta Precision Laboratories, University of Alberta Hospitals, 8440-112 St, Edmonton, Alberta T6G 2J2, Canada |
| authorships[6].author.id | https://openalex.org/A5005497215 |
| authorships[6].author.orcid | https://orcid.org/0000-0002-9173-7299 |
| authorships[6].author.display_name | Michael Joyce |
| authorships[6].countries | CA |
| authorships[6].affiliations[0].institution_ids | https://openalex.org/I154425047 |
| authorships[6].affiliations[0].raw_affiliation_string | Li Ka Shing Institute of Virology, Department of Medical Microbiology and Immunology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2E1, Canada |
| authorships[6].institutions[0].id | https://openalex.org/I154425047 |
| authorships[6].institutions[0].ror | https://ror.org/0160cpw27 |
| authorships[6].institutions[0].type | education |
| authorships[6].institutions[0].lineage | https://openalex.org/I154425047 |
| authorships[6].institutions[0].country_code | CA |
| authorships[6].institutions[0].display_name | University of Alberta |
| authorships[6].author_position | middle |
| authorships[6].raw_author_name | Michael A. Joyce |
| authorships[6].is_corresponding | False |
| authorships[6].raw_affiliation_strings | Li Ka Shing Institute of Virology, Department of Medical Microbiology and Immunology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2E1, Canada |
| authorships[7].author.id | https://openalex.org/A5034114694 |
| authorships[7].author.orcid | |
| authorships[7].author.display_name | Holly A. Saffran |
| authorships[7].countries | CA |
| authorships[7].affiliations[0].institution_ids | https://openalex.org/I154425047 |
| authorships[7].affiliations[0].raw_affiliation_string | Li Ka Shing Institute of Virology, Department of Medical Microbiology and Immunology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2E1, Canada |
| authorships[7].institutions[0].id | https://openalex.org/I154425047 |
| authorships[7].institutions[0].ror | https://ror.org/0160cpw27 |
| authorships[7].institutions[0].type | education |
| authorships[7].institutions[0].lineage | https://openalex.org/I154425047 |
| authorships[7].institutions[0].country_code | CA |
| authorships[7].institutions[0].display_name | University of Alberta |
| authorships[7].author_position | middle |
| authorships[7].raw_author_name | Holly A. Saffran |
| authorships[7].is_corresponding | False |
| authorships[7].raw_affiliation_strings | Li Ka Shing Institute of Virology, Department of Medical Microbiology and Immunology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2E1, Canada |
| authorships[8].author.id | https://openalex.org/A5073147489 |
| authorships[8].author.orcid | https://orcid.org/0000-0002-0983-7267 |
| authorships[8].author.display_name | D. Lorne Tyrrell |
| authorships[8].countries | CA |
| authorships[8].affiliations[0].institution_ids | https://openalex.org/I154425047 |
| authorships[8].affiliations[0].raw_affiliation_string | Li Ka Shing Institute of Virology, Department of Medical Microbiology and Immunology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2E1, Canada |
| authorships[8].institutions[0].id | https://openalex.org/I154425047 |
| authorships[8].institutions[0].ror | https://ror.org/0160cpw27 |
| authorships[8].institutions[0].type | education |
| authorships[8].institutions[0].lineage | https://openalex.org/I154425047 |
| authorships[8].institutions[0].country_code | CA |
| authorships[8].institutions[0].display_name | University of Alberta |
| authorships[8].author_position | middle |
| authorships[8].raw_author_name | D. Lorne Tyrrell |
| authorships[8].is_corresponding | False |
| authorships[8].raw_affiliation_strings | Li Ka Shing Institute of Virology, Department of Medical Microbiology and Immunology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2E1, Canada |
| authorships[9].author.id | https://openalex.org/A5080876972 |
| authorships[9].author.orcid | https://orcid.org/0000-0003-1265-7264 |
| authorships[9].author.display_name | Shawn Babiuk |
| authorships[9].countries | CA |
| authorships[9].affiliations[0].institution_ids | https://openalex.org/I1307613440 |
| authorships[9].affiliations[0].raw_affiliation_string | National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, 1015 Arlington Street, Winnipeg, Manitoba R3E 3M4, Canada |
| authorships[9].institutions[0].id | https://openalex.org/I1307613440 |
| authorships[9].institutions[0].ror | https://ror.org/00qxr8t08 |
| authorships[9].institutions[0].type | government |
| authorships[9].institutions[0].lineage | https://openalex.org/I1307613440 |
| authorships[9].institutions[0].country_code | CA |
| authorships[9].institutions[0].display_name | Canadian Food Inspection Agency |
| authorships[9].author_position | middle |
| authorships[9].raw_author_name | Shawn Babiuk |
| authorships[9].is_corresponding | False |
| authorships[9].raw_affiliation_strings | National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, 1015 Arlington Street, Winnipeg, Manitoba R3E 3M4, Canada |
| authorships[10].author.id | https://openalex.org/A5100707241 |
| authorships[10].author.orcid | https://orcid.org/0000-0003-1088-9862 |
| authorships[10].author.display_name | Hongquan Zhang |
| authorships[10].countries | CA |
| authorships[10].affiliations[0].institution_ids | https://openalex.org/I154425047 |
| authorships[10].affiliations[0].raw_affiliation_string | Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2G3, Canada |
| authorships[10].institutions[0].id | https://openalex.org/I154425047 |
| authorships[10].institutions[0].ror | https://ror.org/0160cpw27 |
| authorships[10].institutions[0].type | education |
| authorships[10].institutions[0].lineage | https://openalex.org/I154425047 |
| authorships[10].institutions[0].country_code | CA |
| authorships[10].institutions[0].display_name | University of Alberta |
| authorships[10].author_position | middle |
| authorships[10].raw_author_name | Hongquan Zhang |
| authorships[10].is_corresponding | True |
| authorships[10].raw_affiliation_strings | Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2G3, Canada |
| authorships[11].author.id | https://openalex.org/A5039488721 |
| authorships[11].author.orcid | https://orcid.org/0000-0002-7690-6701 |
| authorships[11].author.display_name | X. Chris Le |
| authorships[11].countries | CA |
| authorships[11].affiliations[0].institution_ids | https://openalex.org/I154425047 |
| authorships[11].affiliations[0].raw_affiliation_string | Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2G3, Canada |
| authorships[11].institutions[0].id | https://openalex.org/I154425047 |
| authorships[11].institutions[0].ror | https://ror.org/0160cpw27 |
| authorships[11].institutions[0].type | education |
| authorships[11].institutions[0].lineage | https://openalex.org/I154425047 |
| authorships[11].institutions[0].country_code | CA |
| authorships[11].institutions[0].display_name | University of Alberta |
| authorships[11].author_position | last |
| authorships[11].raw_author_name | X. Chris Le |
| authorships[11].is_corresponding | True |
| authorships[11].raw_affiliation_strings | Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2G3, Canada |
| has_content.pdf | True |
| has_content.grobid_xml | True |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.1c03456 |
| open_access.oa_status | bronze |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Integrating Reverse Transcription Recombinase Polymerase Amplification with CRISPR Technology for the One-Tube Assay of RNA |
| has_fulltext | False |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T10878 |
| primary_topic.field.id | https://openalex.org/fields/13 |
| primary_topic.field.display_name | Biochemistry, Genetics and Molecular Biology |
| primary_topic.score | 1.0 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1312 |
| primary_topic.subfield.display_name | Molecular Biology |
| primary_topic.display_name | CRISPR and Genetic Engineering |
| related_works | https://openalex.org/W4291595138, https://openalex.org/W2886212056, https://openalex.org/W3039404550, https://openalex.org/W3090064190, https://openalex.org/W2470941003, https://openalex.org/W4220960485, https://openalex.org/W2914820709, https://openalex.org/W4362521795, https://openalex.org/W3146454725, https://openalex.org/W4389023236 |
| cited_by_count | 110 |
| counts_by_year[0].year | 2025 |
| counts_by_year[0].cited_by_count | 27 |
| counts_by_year[1].year | 2024 |
| counts_by_year[1].cited_by_count | 25 |
| counts_by_year[2].year | 2023 |
| counts_by_year[2].cited_by_count | 28 |
| counts_by_year[3].year | 2022 |
| counts_by_year[3].cited_by_count | 29 |
| counts_by_year[4].year | 2021 |
| counts_by_year[4].cited_by_count | 1 |
| locations_count | 2 |
| best_oa_location.id | doi:10.1021/acs.analchem.1c03456 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S191963794 |
| best_oa_location.source.issn | 0003-2700, 1520-6882 |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | False |
| best_oa_location.source.issn_l | 0003-2700 |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | False |
| best_oa_location.source.display_name | Analytical Chemistry |
| best_oa_location.source.host_organization | https://openalex.org/P4310320006 |
| best_oa_location.source.host_organization_name | American Chemical Society |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310320006 |
| best_oa_location.license | |
| best_oa_location.pdf_url | https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.1c03456 |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | Analytical Chemistry |
| best_oa_location.landing_page_url | https://doi.org/10.1021/acs.analchem.1c03456 |
| primary_location.id | doi:10.1021/acs.analchem.1c03456 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S191963794 |
| primary_location.source.issn | 0003-2700, 1520-6882 |
| primary_location.source.type | journal |
| primary_location.source.is_oa | False |
| primary_location.source.issn_l | 0003-2700 |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | False |
| primary_location.source.display_name | Analytical Chemistry |
| primary_location.source.host_organization | https://openalex.org/P4310320006 |
| primary_location.source.host_organization_name | American Chemical Society |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310320006 |
| primary_location.license | |
| primary_location.pdf_url | https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.1c03456 |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | Analytical Chemistry |
| primary_location.landing_page_url | https://doi.org/10.1021/acs.analchem.1c03456 |
| publication_date | 2021-09-10 |
| publication_year | 2021 |
| referenced_works | https://openalex.org/W2045435533, https://openalex.org/W2064815984, https://openalex.org/W2003171404, https://openalex.org/W2522085729, https://openalex.org/W2606915822, https://openalex.org/W2888908334, https://openalex.org/W2112444085, https://openalex.org/W2787024379, https://openalex.org/W2897700601, https://openalex.org/W2105275554, https://openalex.org/W2153350094, https://openalex.org/W2800905921, https://openalex.org/W3084605929, https://openalex.org/W3017334101, https://openalex.org/W3023764677, https://openalex.org/W2973445868, https://openalex.org/W3024194273, https://openalex.org/W3154788716, https://openalex.org/W3018493306, https://openalex.org/W3087541931, https://openalex.org/W3103998356, https://openalex.org/W3011737892, https://openalex.org/W3189188210, https://openalex.org/W3114523990, https://openalex.org/W4238998302, https://openalex.org/W3102037011, https://openalex.org/W3099840171 |
| referenced_works_count | 27 |
| abstract_inverted_index.H | 133 |
| abstract_inverted_index.S | 185 |
| abstract_inverted_index.a | 37, 49, 53, 152 |
| abstract_inverted_index.20 | 62 |
| abstract_inverted_index.46 | 200 |
| abstract_inverted_index.As | 151 |
| abstract_inverted_index.We | 14, 120, 174, 194 |
| abstract_inverted_index.an | 41, 162 |
| abstract_inverted_index.as | 145 |
| abstract_inverted_index.by | 124 |
| abstract_inverted_index.in | 59, 108 |
| abstract_inverted_index.of | 24, 48, 52, 69, 75, 83, 98, 127, 131, 161, 172, 178, 183, 188, 223, 235, 248 |
| abstract_inverted_index.or | 180 |
| abstract_inverted_index.to | 79, 93, 134, 199 |
| abstract_inverted_index.we | 154 |
| abstract_inverted_index.(40 | 44 |
| abstract_inverted_index.200 | 179 |
| abstract_inverted_index.DNA | 55, 88 |
| abstract_inverted_index.RNA | 77, 136, 190, 249 |
| abstract_inverted_index.RPA | 84, 110, 118, 149 |
| abstract_inverted_index.The | 226 |
| abstract_inverted_index.and | 12, 18, 32, 112, 141, 167, 208, 216, 228, 233, 237, 245 |
| abstract_inverted_index.due | 78 |
| abstract_inverted_index.few | 50 |
| abstract_inverted_index.for | 20, 72, 147, 169, 205, 242 |
| abstract_inverted_index.its | 106 |
| abstract_inverted_index.our | 196 |
| abstract_inverted_index.the | 21, 65, 73, 80, 86, 96, 100, 109, 117, 128, 138, 143, 148, 157, 170, 184, 209, 231 |
| abstract_inverted_index.was | 57, 67 |
| abstract_inverted_index.5-30 | 192 |
| abstract_inverted_index.RNA. | 94, 173 |
| abstract_inverted_index.RPA, | 99 |
| abstract_inverted_index.acid | 5 |
| abstract_inverted_index.both | 9, 212, 243 |
| abstract_inverted_index.cDNA | 144 |
| abstract_inverted_index.free | 142 |
| abstract_inverted_index.from | 137, 211 |
| abstract_inverted_index.gene | 186 |
| abstract_inverted_index.here | 16 |
| abstract_inverted_index.into | 36 |
| abstract_inverted_index.less | 60 |
| abstract_inverted_index.lost | 105 |
| abstract_inverted_index.min. | 63, 193 |
| abstract_inverted_index.more | 181 |
| abstract_inverted_index.rate | 160 |
| abstract_inverted_index.slow | 81 |
| abstract_inverted_index.swab | 203 |
| abstract_inverted_index.than | 61 |
| abstract_inverted_index.tube | 39 |
| abstract_inverted_index.were | 219 |
| abstract_inverted_index.when | 85 |
| abstract_inverted_index.with | 3, 221 |
| abstract_inverted_index.(RNP) | 103 |
| abstract_inverted_index.(RT), | 27 |
| abstract_inverted_index.RNase | 132 |
| abstract_inverted_index.assay | 164, 198 |
| abstract_inverted_index.delay | 97 |
| abstract_inverted_index.lower | 71 |
| abstract_inverted_index.speed | 234 |
| abstract_inverted_index.these | 122 |
| abstract_inverted_index.those | 222 |
| abstract_inverted_index.under | 40 |
| abstract_inverted_index.upper | 201 |
| abstract_inverted_index.using | 165 |
| abstract_inverted_index.viral | 54, 76 |
| abstract_inverted_index.°C). | 45 |
| abstract_inverted_index.(RPA), | 31 |
| abstract_inverted_index.(cDNA) | 89 |
| abstract_inverted_index.During | 95 |
| abstract_inverted_index.RT-RPA | 166, 236 |
| abstract_inverted_index.copies | 51, 182 |
| abstract_inverted_index.issues | 17 |
| abstract_inverted_index.orders | 68 |
| abstract_inverted_index.remove | 135 |
| abstract_inverted_index.showed | 175 |
| abstract_inverted_index.single | 38 |
| abstract_inverted_index.taking | 125 |
| abstract_inverted_index.useful | 241 |
| abstract_inverted_index.within | 191 |
| abstract_inverted_index.RT-qPCR | 224 |
| abstract_inverted_index.applied | 195 |
| abstract_inverted_index.assays, | 239 |
| abstract_inverted_index.hybrids | 140 |
| abstract_inverted_index.improve | 8, 230 |
| abstract_inverted_index.nucleic | 4 |
| abstract_inverted_index.overall | 158 |
| abstract_inverted_index.results | 210 |
| abstract_inverted_index.reverse | 25 |
| abstract_inverted_index.samples | 204 |
| abstract_inverted_index.systems | 1 |
| abstract_inverted_index.COVID-19 | 206 |
| abstract_inverted_index.However, | 64 |
| abstract_inverted_index.RNA-cDNA | 139 |
| abstract_inverted_index.Specific | 46 |
| abstract_inverted_index.achieved | 58 |
| abstract_inverted_index.activity | 107 |
| abstract_inverted_index.analyses | 247 |
| abstract_inverted_index.consumed | 116 |
| abstract_inverted_index.describe | 15 |
| abstract_inverted_index.enhanced | 156 |
| abstract_inverted_index.function | 130 |
| abstract_inverted_index.nuclease | 34 |
| abstract_inverted_index.one-tube | 197 |
| abstract_inverted_index.overcame | 121 |
| abstract_inverted_index.problems | 123 |
| abstract_inverted_index.reaction | 159 |
| abstract_inverted_index.remained | 91 |
| abstract_inverted_index.sequence | 56, 187 |
| abstract_inverted_index.strategy | 227 |
| abstract_inverted_index.template | 90, 146 |
| abstract_inverted_index.advantage | 126 |
| abstract_inverted_index.analysis. | 225 |
| abstract_inverted_index.condition | 43 |
| abstract_inverted_index.detection | 47, 74, 171, 177 |
| abstract_inverted_index.end-point | 217 |
| abstract_inverted_index.gradually | 104 |
| abstract_inverted_index.intensity | 214 |
| abstract_inverted_index.magnitude | 70 |
| abstract_inverted_index.reaction. | 150 |
| abstract_inverted_index.reactions | 35, 115 |
| abstract_inverted_index.reagents. | 119 |
| abstract_inverted_index.solution, | 111 |
| abstract_inverted_index.solutions | 19 |
| abstract_inverted_index.technique | 229 |
| abstract_inverted_index.CRISPR-Cas | 0 |
| abstract_inverted_index.SARS-CoV-2 | 189 |
| abstract_inverted_index.analytical | 10 |
| abstract_inverted_index.consistent | 220 |
| abstract_inverted_index.diagnosis, | 207 |
| abstract_inverted_index.hybridized | 92 |
| abstract_inverted_index.initiation | 82 |
| abstract_inverted_index.integrated | 2, 163 |
| abstract_inverted_index.isothermal | 42 |
| abstract_inverted_index.molecules. | 250 |
| abstract_inverted_index.polymerase | 29 |
| abstract_inverted_index.successful | 22, 176 |
| abstract_inverted_index.techniques | 7 |
| abstract_inverted_index.integration | 23 |
| abstract_inverted_index.nonspecific | 113 |
| abstract_inverted_index.potentially | 240 |
| abstract_inverted_index.recombinase | 28 |
| abstract_inverted_index.respiratory | 202 |
| abstract_inverted_index.sensitivity | 66, 232 |
| abstract_inverted_index.specificity | 11 |
| abstract_inverted_index.consequence, | 153 |
| abstract_inverted_index.crRNA-Cas12a | 101 |
| abstract_inverted_index.fluorescence | 213 |
| abstract_inverted_index.measurements | 215 |
| abstract_inverted_index.sensitivity. | 13 |
| abstract_inverted_index.CRISPR-Cas12a | 33, 168, 238 |
| abstract_inverted_index.amplification | 6, 30, 114 |
| abstract_inverted_index.complementary | 87 |
| abstract_inverted_index.point-of-care | 246 |
| abstract_inverted_index.significantly | 155 |
| abstract_inverted_index.transcription | 26 |
| abstract_inverted_index.visualization | 218 |
| abstract_inverted_index.endoribonuclease | 129 |
| abstract_inverted_index.ribonucleoprotein | 102 |
| abstract_inverted_index.semi-quantitative | 244 |
| cited_by_percentile_year.max | 100 |
| cited_by_percentile_year.min | 89 |
| corresponding_author_ids | https://openalex.org/A5100707241, https://openalex.org/A5039488721 |
| countries_distinct_count | 2 |
| institutions_distinct_count | 12 |
| corresponding_institution_ids | https://openalex.org/I154425047 |
| citation_normalized_percentile.value | 0.98122227 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | True |