Intracellular membrane flow: reconstitution of transition vesicle formation and function in a cell-free system. Article Swipe
D. David Nowack
,
Dorothy M. Morré
,
Mark Paulik
,
T.W. Keenan
,
D. James Morré
·
YOU?
·
· 1987
· Open Access
·
· DOI: https://doi.org/10.1073/pnas.84.17.6098
YOU?
·
· 1987
· Open Access
·
· DOI: https://doi.org/10.1073/pnas.84.17.6098
Transfer of membrane between endoplasmic reticulum and Golgi apparatus in situ is considered to occur via 60-nm transition vesicles derived from part-rough, part-smooth transition elements of the endoplasmic reticulum. A procedure is described for the isolation of a fraction enriched in these transition elements from rat liver. The isolated fraction generates small vesicles morphologically resembling transition vesicles when incubated with nucleoside triphosphate at 37 degrees C. In the cell-free system consisting of a donor fraction enriched in transition elements and an acceptor fraction consisting of intact Golgi apparatus immobilized on nitrocellulose strips, transfer in vitro of radiolabeled membranes was demonstrated. Nucleoside triphosphates were required for transfer, and transfer was facilitated by a cytosol fraction of Mr greater than 10,000. In the presence of both nucleoside triphosphate and cytosol, radiolabeled proteins were transferred in a manner dependent upon both time and temperature. Transfer appeared to be both vectorial and specific in that, with Golgi apparatus (or endoplasmic reticulum) as both donor and acceptor, only negligible time and temperature-dependent transfer was observed. The test system described is expected to facilitate further investigation of the transfer process and to provide a convenient assay to guide transition vesicle isolation and characterization.
Related Topics
Concepts
Endoplasmic reticulum
Vesicle
Golgi apparatus
Nucleoside triphosphate
Membrane
Biophysics
Cytosol
Biochemistry
Chemistry
Biology
Nucleotide
Enzyme
Gene
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1073/pnas.84.17.6098
- OA Status
- green
- Cited By
- 53
- References
- 13
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2051557577
All OpenAlex metadata
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https://openalex.org/W2051557577Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1073/pnas.84.17.6098Digital Object Identifier
- Title
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Intracellular membrane flow: reconstitution of transition vesicle formation and function in a cell-free system.Work title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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1987Year of publication
- Publication date
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1987-09-01Full publication date if available
- Authors
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D. David Nowack, Dorothy M. Morré, Mark Paulik, T.W. Keenan, D. James MorréList of authors in order
- Landing page
-
https://doi.org/10.1073/pnas.84.17.6098Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
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https://www.ncbi.nlm.nih.gov/pmc/articles/299015Direct OA link when available
- Concepts
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Endoplasmic reticulum, Vesicle, Golgi apparatus, Nucleoside triphosphate, Membrane, Biophysics, Cytosol, Biochemistry, Chemistry, Biology, Nucleotide, Enzyme, GeneTop concepts (fields/topics) attached by OpenAlex
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53Total citation count in OpenAlex
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2016: 1Per-year citation counts (last 5 years)
- References (count)
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13Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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