Isolation and Characterization of Porcine Endocardial Endothelial Cells Article Swipe
Kathleen N. Brown
,
Hong Kim T. Phan
,
Elysa Jui
,
Marci K. Kang
,
Jennifer P. Connell
,
Sundeep G. Keswani
,
K. Jane Grande‐Allen
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1089/ten.tec.2023.0009
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1089/ten.tec.2023.0009
The heart contains diverse endothelial cell types. We sought to characterize the endocardial endothelial cells (EECs), which line the chambers of the heart. EECs are relatively understudied, yet their dysregulation can lead to various cardiac pathologies. Due to the lack of commercial availability of these cells, we reported our protocol for isolating EECs from porcine hearts and for establishing an EEC population through cell sorting. In addition, we compared the EEC phenotype and fundamental behaviors to a well-studied endothelial cell line, human umbilical vein endothelial cells (HUVECs). The EECs stained positively for classic phenotypic markers such as CD31, von Willebrand Factor, and vascular endothelial (VE) cadherin. The EECs proliferated more quickly than HUVECs at 48 h (1310 ± 251 cells vs. 597 ± 130 cells, p = 0.0361) and at 96 h (2873 ± 257 cells vs. 1714 ± 342 cells, p = 0.0002). Yet EECs migrated more slowly than HUVECs to cover a scratch wound at 4 h (5% ± 1% wound closure vs. 25% ± 3% wound closure, p < 0.0001), 8 h (15% ± 4% wound closure vs. 51% ± 12% wound closure, p < 0.0001), and 24 h (70% ± 11% wound closure vs. 90% ± 3% wound closure, p < 0.0001). Finally, the EECs maintained their endothelial phenotype by positive expression of CD31 through more than a dozen passages (three populations of EECs showing 97% ± 1% CD31+ cells in over 14 passages). In contrast, the HUVECs showed significantly reduced CD31 expression over high passages (80% ± 11% CD31+ cells over 14 passages). These important phenotypic differences between EECs and HUVECs highlight the need for researchers to utilize the most relevant cell types when studying or modeling diseases of interest.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1089/ten.tec.2023.0009
- OA Status
- green
- Cited By
- 1
- References
- 40
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4380433768
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4380433768Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1089/ten.tec.2023.0009Digital Object Identifier
- Title
-
Isolation and Characterization of Porcine Endocardial Endothelial CellsWork title
- Type
-
articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2023Year of publication
- Publication date
-
2023-06-13Full publication date if available
- Authors
-
Kathleen N. Brown, Hong Kim T. Phan, Elysa Jui, Marci K. Kang, Jennifer P. Connell, Sundeep G. Keswani, K. Jane Grande‐AllenList of authors in order
- Landing page
-
https://doi.org/10.1089/ten.tec.2023.0009Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
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https://pmc.ncbi.nlm.nih.gov/articles/PMC10442675/pdf/ten.tec.2023.0009.pdfDirect OA link when available
- Concepts
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CD31, Umbilical vein, Wound healing, Endothelial stem cell, Population, Cell biology, Biology, Cell culture, Phenotype, Immunology, Medicine, Angiogenesis, Cancer research, In vitro, Genetics, Gene, Environmental healthTop concepts (fields/topics) attached by OpenAlex
- Cited by
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1Total citation count in OpenAlex
- Citations by year (recent)
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2025: 1Per-year citation counts (last 5 years)
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40Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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