Jurkat T Cell Detectability and Toxicity Evaluation of Low-Temperature Synthesized Cadmium Quantum Dots Article Swipe
Ngoc Thuy Vo
,
Vũ Thị Ngọc Bích
,
Hong Tran Thi Diep
,
Thien Le Khanh
,
Hieu Tran Van
,
Thanh Bình Nguyễn
,
Quang Vinh Lam
·
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1155/2020/9346423
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1155/2020/9346423
Early and highly accurate detection of diverse diseases is in urgent demand than ever, especially for cancers and infectious ones. Among possibilities, biosensing by utilizing conjugated nanoparticles is still a method of choice. However, the toxicity of quantum dots remains a big matter of concern in those biooriented applications. In this study, mercaptosuccinic acid-coated cadmium selenide quantum dots of approximately 2.3 nm were synthesized with a simple green method at low temperature and cost-saving chemicals. The influence of synthesis factors was investigated with different spectroscopic methods. The toxicity issue was evaluated on the NIH-3T3 cell line (ATCC® CRL-1658™) and an MTT assay, revealing a secure threshold of 20 μ g/ml. Consequently, successful conjugation to the CD3 antibody including an A/G protein bridge was implemented and verified with fluorescent methods. Finally, Jurkat T cell detectability of conjugated CdSe was successfully validated with fluorescent microscopy. The CdSe-based products are accessible for future biosensing applications.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1155/2020/9346423
- http://downloads.hindawi.com/journals/jnm/2020/9346423.pdf
- OA Status
- hybrid
- Cited By
- 1
- References
- 25
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3035493392
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- OpenAlex ID
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https://openalex.org/W3035493392Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1155/2020/9346423Digital Object Identifier
- Title
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Jurkat T Cell Detectability and Toxicity Evaluation of Low-Temperature Synthesized Cadmium Quantum DotsWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
-
2020Year of publication
- Publication date
-
2020-06-12Full publication date if available
- Authors
-
Ngoc Thuy Vo, Vũ Thị Ngọc Bích, Hong Tran Thi Diep, Thien Le Khanh, Hieu Tran Van, Thanh Bình Nguyễn, Quang Vinh LamList of authors in order
- Landing page
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https://doi.org/10.1155/2020/9346423Publisher landing page
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https://downloads.hindawi.com/journals/jnm/2020/9346423.pdfDirect link to full text PDF
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YesWhether a free full text is available
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hybridOpen access status per OpenAlex
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https://downloads.hindawi.com/journals/jnm/2020/9346423.pdfDirect OA link when available
- Concepts
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Quantum dot, Jurkat cells, Cadmium selenide, Materials science, Cadmium, Conjugated system, Nanotechnology, Biosensor, Fluorescence, Toxicity, Nanoparticle, Chemistry, T cell, Physics, Polymer, Optics, Organic chemistry, Biology, Metallurgy, Composite material, Immune system, ImmunologyTop concepts (fields/topics) attached by OpenAlex
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1Total citation count in OpenAlex
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2023: 1Per-year citation counts (last 5 years)
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25Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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