Keragaman Genetika Empat Belas Aksesi Kentang (Solanum tuberosum L.) Berdasarkan Marka SSR dan STS (Genetic Diversity of Fourteen Potato Accessions Based on SSR and STS Markers) Article Swipe
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.21082/jbio.v11n2.2015.p41-48
· OA: W2555393055
<p>Potato is one of high economically horticultural plant. The increasing of national consumption of potato becomes a challenge for<br />potato breeders. The success of breeding programs is depending on availability of genetic diversity. The aim of this research was<br />to analyze the genetic diversity of fourteen accessions of potato by using SSR and STS markers. PCR analysis was scored as biner<br />data and the collected data was analyzed using NTSYS and PowerMarker. The result showed that there were 63% polymorphic<br />(12 markers) of total markers. As many as 60 alleles with the size of 200–500 bp were identified by a range of 2–9 alleles per<br />locus. The polymorphism level was 0.59 (0.36–0.74). Result also showed the average of major allele frequency was 49.42%<br />(35.71–63.64%). Nine markers which have polymorphism level more than 0.5 could be used to detect genetic diversity of potato.<br />The average of genetic diversity index was 0.65. Cluster analysis showed that 14 accessions of potato were split in two groups<br />(coefficient 0.70). The first groups consisted of Atlantik, GM 05, Granola Kembang Merbabu 17, and the second groups consist of<br />Repita, Maglia, Medians, CIP397078.7, CIP392781.1, Margahayu, Granola, CIP394613.139, Amabile, and Tenggo. The information<br />of genetic diversity of this germplasm could be used as a preliminary basis for choosing crossing parents in potato breeding in<br />Indonesia.</p>
