Light-Induced Orthogonal Fragmentation of Crosslinked Peptides Article Swipe
Lars Kolbowski
,
Adam Belsom
,
Ana M. Pérez‐López
,
Tony Ly
,
Juri Rappsilber
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1021/jacsau.3c00199
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1021/jacsau.3c00199
Crosslinking mass spectrometry provides pivotal information on the structure and interaction of proteins. MS-cleavable crosslinkers are regarded as a cornerstone for the analysis of complex mixtures. Yet they fragment under similar conditions as peptides, leading to mixed fragmentation spectra of the crosslinker and peptide. This hampers selecting individual peptides for their independent identification. Here, we introduce orthogonal cleavage using ultraviolet photodissociation (UVPD) to increase crosslinker over peptide fragmentation. We designed and synthesized a crosslinker that can be cleaved at 213 nm in a commercial mass spectrometer configuration. In an analysis of crosslinked Escherichia coli lysate, the crosslinker-to-peptide fragment intensity ratio increases from nearly 1 for a conventionally cleavable crosslinker to 5 for the UVPD-cleavable crosslinker. This largely increased the sensitivity of selecting the individual peptides for MS3, even more so with an improved doublet detection algorithm. Data are available via ProteomeXchange with identifier PXD040267.
Related Topics
Concepts
Peptide
Photodissociation
Fragmentation (computing)
Chemistry
Mass spectrometry
Cleavage (geology)
Combinatorial chemistry
Ultraviolet
Biophysics
Chromatography
Photochemistry
Biochemistry
Materials science
Computer science
Biology
Optoelectronics
Operating system
Fracture (geology)
Composite material
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1021/jacsau.3c00199
- OA Status
- gold
- Cited By
- 2
- References
- 27
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4385951464
All OpenAlex metadata
Raw OpenAlex JSON
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https://openalex.org/W4385951464Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1021/jacsau.3c00199Digital Object Identifier
- Title
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Light-Induced Orthogonal Fragmentation of Crosslinked PeptidesWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2023Year of publication
- Publication date
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2023-08-17Full publication date if available
- Authors
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Lars Kolbowski, Adam Belsom, Ana M. Pérez‐López, Tony Ly, Juri RappsilberList of authors in order
- Landing page
-
https://doi.org/10.1021/jacsau.3c00199Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
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https://doi.org/10.1021/jacsau.3c00199Direct OA link when available
- Concepts
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Peptide, Photodissociation, Fragmentation (computing), Chemistry, Mass spectrometry, Cleavage (geology), Combinatorial chemistry, Ultraviolet, Biophysics, Chromatography, Photochemistry, Biochemistry, Materials science, Computer science, Biology, Optoelectronics, Operating system, Fracture (geology), Composite materialTop concepts (fields/topics) attached by OpenAlex
- Cited by
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2Total citation count in OpenAlex
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2025: 1, 2024: 1Per-year citation counts (last 5 years)
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27Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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