Light induces rapid changes of the phosphorylation pattern in the cytosol of evacuolated parsley protoplasts. Article Swipe
Klaus Harter
,
Hanns Frohnmeyer
,
Stefan Kircher
,
Tim Kunkel
,
Susanne Mühlbauer
,
E. Schäfer
·
YOU?
·
· 1994
· Open Access
·
· DOI: https://doi.org/10.1073/pnas.91.11.5038
YOU?
·
· 1994
· Open Access
·
· DOI: https://doi.org/10.1073/pnas.91.11.5038
The fractionation of cells of a parsley suspension culture [Petroselinum crispum (Mill.) A. Hill] by protoplasting and subsequent removal of the vacuoles led to physiologically intact evacuolated protoplasts retaining light inducibility of chalcone synthase expression. Lysis of the evacuolated protoplasts permitted the isolation of a pure, highly concentrated cytosolic fraction containing major cytosolic membranes but only minor contamination by proplastids, mitochondria, and nuclei. Short-time irradiations of the cytosol with red or UV-containing white light resulted in very fast changes of the phosphorylation pattern of 18-, 40-, 48-, 55- to 70-, and 120-kDa proteins. Major differences were observed between the phosphorylation patterns obtained by red or UV-containing white light treatment, indicating a different primary action of the excited photoreceptors in vitro. Separation of the microsomal fraction from the cytosolic matrix established the localization of these proteins. Chase and photoreversibility experiments revealed that phytochrome in vitro regulates the phosphorylation of the 40-kDa protein by modifying a soluble cytosolic kinase/phosphatase system.
Related Topics
Concepts
Cytosol
Biology
Biochemistry
Phosphorylation
Protein phosphorylation
Phosphatase
Cell fractionation
Protein kinase A
Cell biology
Enzyme
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1073/pnas.91.11.5038
- OA Status
- green
- Cited By
- 51
- References
- 20
- Related Works
- 10
- OpenAlex ID
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All OpenAlex metadata
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https://openalex.org/W2090700791Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1073/pnas.91.11.5038Digital Object Identifier
- Title
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Light induces rapid changes of the phosphorylation pattern in the cytosol of evacuolated parsley protoplasts.Work title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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1994Year of publication
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1994-05-24Full publication date if available
- Authors
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Klaus Harter, Hanns Frohnmeyer, Stefan Kircher, Tim Kunkel, Susanne Mühlbauer, E. SchäferList of authors in order
- Landing page
-
https://doi.org/10.1073/pnas.91.11.5038Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
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greenOpen access status per OpenAlex
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https://www.ncbi.nlm.nih.gov/pmc/articles/43926Direct OA link when available
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Cytosol, Biology, Biochemistry, Phosphorylation, Protein phosphorylation, Phosphatase, Cell fractionation, Protein kinase A, Cell biology, EnzymeTop concepts (fields/topics) attached by OpenAlex
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51Total citation count in OpenAlex
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2023: 2, 2020: 1, 2016: 1Per-year citation counts (last 5 years)
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10Other works algorithmically related by OpenAlex
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