Limited, but potentially functional translation of non-coding transcripts in the HEK293T cellular cytosol Article Swipe
Annelies Bogaert
,
Daria Fijałkowska
,
An Staes
,
Tessa Van de Steene
,
Hans Demol
,
Kris Gevaert
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1101/2021.12.23.473848
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1101/2021.12.23.473848
Ribosome profiling has revealed translation outside of canonical coding sequences (CDSs) including translation of short upstream ORFs, long non-coding RNAs, overlapping ORFs, ORFs in UTRs or ORFs in alternative reading frames. Studies combining mass spectrometry, ribosome profiling and CRISPR-based screens showed that hundreds of ORFs derived from non-coding transcripts produce (micro)proteins, while other studies failed to find evidence for such types of non-canonical translation products. Here, we attempted to discover translation products from non-coding regions by strongly reducing the complexity of the sample prior to mass spectrometric analysis. We used an extended database as the search space and applied stringent filtering of the identified peptides to find evidence for novel translation events. Theoretically, we show that our strategy facilitates the detection of translation events of transcripts from non-coding regions, but experimentally only find 19 peptides (less than 1% of all identified peptides) that might originate from such translation events. Virotrap based interactome analysis of two N-terminal proteoforms originating from non-coding regions finally showed the functional potential of these novel proteins.
Related Topics
Concepts
ORFS
Ribosome profiling
Open reading frame
Computational biology
Biology
Translation (biology)
Interactome
Ribosome
Coding region
Genetics
Messenger RNA
Gene
RNA
Peptide sequence
Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2021.12.23.473848
- https://www.biorxiv.org/content/biorxiv/early/2021/12/23/2021.12.23.473848.full.pdf
- OA Status
- green
- References
- 85
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4200038303
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4200038303Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1101/2021.12.23.473848Digital Object Identifier
- Title
-
Limited, but potentially functional translation of non-coding transcripts in the HEK293T cellular cytosolWork title
- Type
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preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
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2021Year of publication
- Publication date
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2021-12-23Full publication date if available
- Authors
-
Annelies Bogaert, Daria Fijałkowska, An Staes, Tessa Van de Steene, Hans Demol, Kris GevaertList of authors in order
- Landing page
-
https://doi.org/10.1101/2021.12.23.473848Publisher landing page
- PDF URL
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https://www.biorxiv.org/content/biorxiv/early/2021/12/23/2021.12.23.473848.full.pdfDirect link to full text PDF
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YesWhether a free full text is available
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greenOpen access status per OpenAlex
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https://www.biorxiv.org/content/biorxiv/early/2021/12/23/2021.12.23.473848.full.pdfDirect OA link when available
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ORFS, Ribosome profiling, Open reading frame, Computational biology, Biology, Translation (biology), Interactome, Ribosome, Coding region, Genetics, Messenger RNA, Gene, RNA, Peptide sequenceTop concepts (fields/topics) attached by OpenAlex
- Cited by
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0Total citation count in OpenAlex
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85Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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