Lipid nanoparticles as a tool to dissect dendritic cell maturation pathways Article Swipe
Sophie Janssens
,
Sofie Rennen
,
Victor Bosteels
,
Clint De Nolf
,
Karo Van Lil
,
Sandra Maréchal
,
Jessica Vetters
,
Evelien Van De Velde
,
Farzaneh Fayazpour
,
Ria Roelandt
,
Niels Vandamme
,
Kevin Verstaen
,
Stefaan C. De Smedt
,
Karine Breckpot
,
Bruno De Geest
,
Rein Verbeke
,
Ine Lentacker
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.21203/rs.3.rs-5461735/v1
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.21203/rs.3.rs-5461735/v1
Dendritic cells (DCs) are short-lived immune cells that continuously roam our body in search for foreign or self-antigens. Upon acquisition of antigen, they mature and start migrating to the lymph node to present the antigen to naïve T cells. Depending on the context wherein the antigen is acquired, DCs will mature in a homeostatic or immunogenic manner. So far, the field is lacking proper tools to distinguish between the two maturation states. Most maturation markers are shared between the two states and therefore inappropriate to use. Still, defining the proper maturation type is crucial as it determines how the DCs will instruct the T cells towards antigen expressing cells. In this study, we used a lipid nanoparticle (LNP)-based approach to steer DC maturation pathways in vivo. CITE-seq analysis allowed us to design a panel of flow cytometry markers that reliably annotates the two DC maturation states, as validated in an infection and in a tumor model. Furthermore, the data corroborated that uptake of empty LNPs in DCs induces their homeostatic maturation, in contrast to uptake of mRNA-LNPs or TLR ligand-adjuvanted LNPs, leading to distinct effector T cell outputs. This reveals that LNPs themselves are not being decoded as “danger” by cDC1s, and that the cargo is essential to provide adjuvants activity, which is highly relevant for targeted design of LNP-based therapies.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.21203/rs.3.rs-5461735/v1
- https://www.researchsquare.com/article/rs-5461735/latest.pdf
- OA Status
- gold
- References
- 126
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4404868006
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4404868006Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.21203/rs.3.rs-5461735/v1Digital Object Identifier
- Title
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Lipid nanoparticles as a tool to dissect dendritic cell maturation pathwaysWork title
- Type
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preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
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2024Year of publication
- Publication date
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2024-11-29Full publication date if available
- Authors
-
Sophie Janssens, Sofie Rennen, Victor Bosteels, Clint De Nolf, Karo Van Lil, Sandra Maréchal, Jessica Vetters, Evelien Van De Velde, Farzaneh Fayazpour, Ria Roelandt, Niels Vandamme, Kevin Verstaen, Stefaan C. De Smedt, Karine Breckpot, Bruno De Geest, Rein Verbeke, Ine LentackerList of authors in order
- Landing page
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https://doi.org/10.21203/rs.3.rs-5461735/v1Publisher landing page
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https://www.researchsquare.com/article/rs-5461735/latest.pdfDirect link to full text PDF
- Open access
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YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://www.researchsquare.com/article/rs-5461735/latest.pdfDirect OA link when available
- Concepts
-
Cell biology, Dendritic cell, Computational biology, Chemistry, Lipid signaling, Cell, Biology, Immunology, Biochemistry, Immune system, EnzymeTop concepts (fields/topics) attached by OpenAlex
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0Total citation count in OpenAlex
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126Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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