Live-cell imaging of circular and long noncoding RNAs associated with FUS pathological aggregates by Pepper fluorescent RNA Article Swipe
Erika Vitiello
,
Francesco Castagnetti
,
Lorenzo Stufera Mecarelli
,
Eleonora D’Ambra
,
Paolo Tollis
,
Giancarlo Ruocco
,
Pietro Laneve
,
Elisa Caffarelli
,
Davide Mariani
,
Irene Bozzoni
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1261/rna.080119.124
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1261/rna.080119.124
Lately, important advancements in visualizing RNAs in fixed and live cells have been achieved. Although mRNA imaging techniques are well-established, the development of effective methods for studying noncoding RNAs (ncRNAs) in living cells is still challenging but necessary, as they show a variety of functions and intracellular localizations, including participation in highly dynamic processes like phase transition, which is still poorly studied in vivo. Addressing this issue, we tagged two exemplary ncRNAs with the fluorescent RNA (fRNA) Pepper. Specifically, we showed that circ-HDGFRP3 interacts with p-bodies and is recruited in pathological FUS aggregates in a dynamic fashion, and we super-resolved its distribution in such condensates via structured illumination microscopy. Moreover, we tracked the long noncoding RNA (lncRNA) nHOTAIRM1, a motor neuron–specific constituent of stress granules, monitoring its behavior throughout the oxidative-stress response in physiological and pathological conditions. Overall, as fRNA development progresses, our work demonstrates an effective use of Pepper for monitoring complex processes, such as phase transition, in living cells through the visualization of circular RNAs (circRNAs) and lncRNAs with super-resolution power.
Related Topics
Concepts
Biology
RNA
Cell biology
Non-coding RNA
microRNA
Transfection
Stress granule
Cell
Intracellular
Computational biology
Messenger RNA
Gene
Genetics
Translation (biology)
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1261/rna.080119.124
- http://rnajournal.cshlp.org/content/early/2025/01/08/rna.080119.124.full.pdf
- OA Status
- hybrid
- Cited By
- 1
- References
- 77
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4406164619
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4406164619Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1261/rna.080119.124Digital Object Identifier
- Title
-
Live-cell imaging of circular and long noncoding RNAs associated with FUS pathological aggregates by Pepper fluorescent RNAWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2025Year of publication
- Publication date
-
2025-01-08Full publication date if available
- Authors
-
Erika Vitiello, Francesco Castagnetti, Lorenzo Stufera Mecarelli, Eleonora D’Ambra, Paolo Tollis, Giancarlo Ruocco, Pietro Laneve, Elisa Caffarelli, Davide Mariani, Irene BozzoniList of authors in order
- Landing page
-
https://doi.org/10.1261/rna.080119.124Publisher landing page
- PDF URL
-
https://rnajournal.cshlp.org/content/early/2025/01/08/rna.080119.124.full.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
hybridOpen access status per OpenAlex
- OA URL
-
https://rnajournal.cshlp.org/content/early/2025/01/08/rna.080119.124.full.pdfDirect OA link when available
- Concepts
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Biology, RNA, Cell biology, Non-coding RNA, microRNA, Transfection, Stress granule, Cell, Intracellular, Computational biology, Messenger RNA, Gene, Genetics, Translation (biology)Top concepts (fields/topics) attached by OpenAlex
- Cited by
-
1Total citation count in OpenAlex
- Citations by year (recent)
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2025: 1Per-year citation counts (last 5 years)
- References (count)
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77Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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