LuminoCell: a versatile and affordable luminometer platform for monitoring in-cell luciferase-based reporters Article Swipe
Kamila Weissová
,
Bohumil Fafílek
,
Tomasz Radaszkiewicz
,
Canan Celiker
,
Petra Macháčková
,
Tamara Čechová
,
Jana Šebestíková
,
Aleš Hampl
,
Vı́tězslav Bryja
,
Pavel Krejčı́
,
Tomáš Bárta
·
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1101/2022.01.28.478134
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1101/2022.01.28.478134
Luciferase reporter assays represent a simple and sensitive experimental system in cell and molecular biology to study multiple biological processes. However, the application of these assays is often limited by the costs of conventional luminometer instruments and the versatility of their use in different experimental conditions. Therefore, we aimed to develop a small, affordable luminometer allowing continuous measurement of luciferase activity, designed for inclusion into various kinds of tissue culture incubators. Here we introduce LuminoCell - an open-source platform for the construction of an affordable, sensitive, and portable luminometer capable of realtime monitoring in-cell luciferase activity. The LuminoCell costs $40, requires less than 1 hour to assemble, and it is capable of performing real-time sensitive detection of both magnitude and duration of the activity of major signalling pathways in cell cultures, including receptor tyrosine kinases (EGF, FGF), WNT/β-catenin, and NF-κB. Additionally, we show that the LuminoCell is suitable to be used in cytotoxicity assays as well as for monitoring periodic circadian gene expression.
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- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2022.01.28.478134
- https://www.biorxiv.org/content/biorxiv/early/2022/01/28/2022.01.28.478134.full.pdf
- OA Status
- green
- Cited By
- 1
- References
- 22
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4210552615
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4210552615Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1101/2022.01.28.478134Digital Object Identifier
- Title
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LuminoCell: a versatile and affordable luminometer platform for monitoring in-cell luciferase-based reportersWork title
- Type
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preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
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2022Year of publication
- Publication date
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2022-01-28Full publication date if available
- Authors
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Kamila Weissová, Bohumil Fafílek, Tomasz Radaszkiewicz, Canan Celiker, Petra Macháčková, Tamara Čechová, Jana Šebestíková, Aleš Hampl, Vı́tězslav Bryja, Pavel Krejčı́, Tomáš BártaList of authors in order
- Landing page
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https://doi.org/10.1101/2022.01.28.478134Publisher landing page
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https://www.biorxiv.org/content/biorxiv/early/2022/01/28/2022.01.28.478134.full.pdfDirect link to full text PDF
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YesWhether a free full text is available
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greenOpen access status per OpenAlex
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https://www.biorxiv.org/content/biorxiv/early/2022/01/28/2022.01.28.478134.full.pdfDirect OA link when available
- Concepts
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Luciferase, Computational biology, Wnt signaling pathway, Cell biology, Chemistry, Cell culture, Computer science, Biology, Signal transduction, Genetics, TransfectionTop concepts (fields/topics) attached by OpenAlex
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1Total citation count in OpenAlex
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2025: 1Per-year citation counts (last 5 years)
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10Other works algorithmically related by OpenAlex
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