Lyophilized human platelet lysate: manufacturing, quality control, and application Article Swipe
Kerstin Wendland
,
Lea Koblin
,
Dirk Stobbe
,
A Dahms
,
Debora Singer
,
Sander Bekeschus
,
Jan Wesche
,
Janosch Schoon
,
Konstanze Aurich
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.3389/fcell.2025.1513444
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.3389/fcell.2025.1513444
Background A significant number of platelet concentrates (PCs) is discarded daily in blood banks due to limited shelf life. Human platelet lysate (HPL), derived from expired PCs, has gained attention as an ethical and sustainable cell culture media supplement in biomedical research and cell therapy production. However, HPL is subject to decisive disadvantages such as batch differences and lack of storage stability. To overcome these limitations and to enhance the applicability of HPL, we developed an HPL manufacturing protocol including a lyophilization process. The aim of this study was to investigate the influence of HPL lyophilization on parameters of quality control, including growth factor concentrations and the culture of human mesenchymal stromal cells (hMSCs). Methods We performed a paired comparison of six batches of HPL and lyophilized HPL (L-HPL) regarding the quality parameters pH, total protein, osmolality, sodium, potassium and chloride concentration. Concentrations of 11 growth factors and cytokines were compared between HPL and L-HPL. Additionally, we determined cell yield, proliferation capacity, viability and trilineage differentiation potential of hMSCs following expansion in HPL- and L-HPL-supplemented cell culture media. Results Quantification of the quality parameters revealed non-altered pH, osmolality and potassium concentrations and slightly lower total protein, sodium and chloride concentrations of L-HPL compared to HPL. Growth factor and cytokine concentrations did not differ between HPL and L-HPL. Cell yield, division cycles and viability of hMSCs cultured in either HPL- or L-HPL-containing media were comparable. Cells differentiated in medium containing L-HPL showed a slightly higher capacity for osteogenic differentiation, while adipogenic differentiation and chondrogenic differentiation potentials remained unchanged. Conclusion We successfully developed a method to produce well-applicable L-HPL. The comparison of L-HPL with HPL did not reveal any relevant differences regarding quality control parameters of routine testing, growth factor concentrations and hMSC functionality, demonstrating the suitability of L-HPL as a cell culture supplement. These results emphasize the potential of L-HPL as a sustainable and ethical alternative to animal-derived serum products in biomedical research and drug development.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3389/fcell.2025.1513444
- OA Status
- gold
- Cited By
- 2
- References
- 29
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4406850282
All OpenAlex metadata
Raw OpenAlex JSON
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https://openalex.org/W4406850282Canonical identifier for this work in OpenAlex
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https://doi.org/10.3389/fcell.2025.1513444Digital Object Identifier
- Title
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Lyophilized human platelet lysate: manufacturing, quality control, and applicationWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2025Year of publication
- Publication date
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2025-01-27Full publication date if available
- Authors
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Kerstin Wendland, Lea Koblin, Dirk Stobbe, A Dahms, Debora Singer, Sander Bekeschus, Jan Wesche, Janosch Schoon, Konstanze AurichList of authors in order
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https://doi.org/10.3389/fcell.2025.1513444Publisher landing page
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YesWhether a free full text is available
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goldOpen access status per OpenAlex
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https://doi.org/10.3389/fcell.2025.1513444Direct OA link when available
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Chemistry, Cell growth, Cell culture, Growth factor, Mesenchymal stem cell, Viability assay, Cell, Biochemistry, Biology, Cell biology, Receptor, GeneticsTop concepts (fields/topics) attached by OpenAlex
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2Total citation count in OpenAlex
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10Other works algorithmically related by OpenAlex
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