Manipulatingearly pig embryos Article Swipe
Heiner Niemann
,
B. Reichelt
·
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1530/biosciprocs.14.006
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1530/biosciprocs.14.006
On the basis of established surgical procedures for embryo recovery and transfer, the early pig embryo can be subjected to various manipulations aimed at a longterm preservation of genetic material, the generation of identical multiplets, the early determination of sex or the alteration of the genetic make-up. Most of these procedures are still at an experimental stage and despite recent considerable progress are far from practical application. Normal piglets have been obtained after cryopreservation of pig blastocysts hatched in vitro, whereas all attempts to freeze embryos with intact zona pellucida have been unsuccessful. Pig embryos at the morula and blastocyst stage can be bisected microsurgically and the resulting demiembryos possess a high developmental potential in vitro, whereas their development in vivo is impaired. Pregnancy rates are similar (80%) but litter size is reduced compared with intact embryos and twinning rate is approximately 2%. Pig blastomeres isolated from embryos up to the 16-cell stage can be grown in culture and result in normal blastocysts. Normal piglets have been bom upon transfer of blastocysts derived from isolated eight-cell blastomeres, clearly underlining the totipotency of this developmental stage. Upon nuclear transfer the developmental capacity of reconstituted pig embryos is low and < 10% develop to morulae or blastocysts in vitro. Pig oocytes can be stimulated parthenogenetically and up to 10% grow to blastocysts in the in vitro culture. Sex determination can be achieved either by separation of X and Y chromosome bearing spermatozoa by flow cytometry or by analysing the expression of the HY antigen in pig embryos from the eight-cell to morula stage. Microinjection of foreign DNA has been successfully used to alter growth and development of transgenic pigs, and to produce foreign proteins in the mammary gland or in the bloodstream, indicating that pigs can be used as donors for valuable human pharmaceutical proteins. Another promising area of gene transfer is the increase of disease resistance in transgenic lines of pigs. Approximately 30% of pig spermatozoa bind considerable amounts of foreign DNA preferably at the post-acrosomal region, suggesting that transgenic animals can be obtained more efficiently than with the usual microinjection procedure. To increase gene transfer efficiency, considerable research efforts have been made to establish embryonic stem (ES) cells, but so far there is no definite proof of totipotency of the generated pig ESlike cells through viable chimaeras. In general, biotechnological procedures are much less advanced in pigs than in cows.
Related Topics
Concepts
Blastomere
Embryo
Andrology
Biology
Blastocyst
Zona pellucida
Embryo transfer
Cryopreservation
Embryo culture
Embryogenesis
Genetics
Oocyte
Medicine
Metadata
- Type
- article
- Language
- it
- Landing Page
- https://doi.org/10.1530/biosciprocs.14.006
- https://www.biosciproceedings.org//bp/0014/bp0014cpr6.pdf
- OA Status
- diamond
- References
- 4
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3092030730
All OpenAlex metadata
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- OpenAlex ID
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https://openalex.org/W3092030730Canonical identifier for this work in OpenAlex
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https://doi.org/10.1530/biosciprocs.14.006Digital Object Identifier
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Manipulatingearly pig embryosWork title
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articleOpenAlex work type
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itPrimary language
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2020Year of publication
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2020-10-12Full publication date if available
- Authors
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Heiner Niemann, B. ReicheltList of authors in order
- Landing page
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https://doi.org/10.1530/biosciprocs.14.006Publisher landing page
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https://www.biosciproceedings.org//bp/0014/bp0014cpr6.pdfDirect link to full text PDF
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YesWhether a free full text is available
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diamondOpen access status per OpenAlex
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https://www.biosciproceedings.org//bp/0014/bp0014cpr6.pdfDirect OA link when available
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Blastomere, Embryo, Andrology, Biology, Blastocyst, Zona pellucida, Embryo transfer, Cryopreservation, Embryo culture, Embryogenesis, Genetics, Oocyte, MedicineTop concepts (fields/topics) attached by OpenAlex
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0Total citation count in OpenAlex
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4Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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| abstract_inverted_index.pigs. | 319 |
| abstract_inverted_index.proof | 375 |
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| abstract_inverted_index.stage | 56, 100, 152 |
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| abstract_inverted_index.their | 117 |
| abstract_inverted_index.there | 371 |
| abstract_inverted_index.these | 49 |
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| abstract_inverted_index.vitro | 223 |
| abstract_inverted_index.ESlike | 382 |
| abstract_inverted_index.Normal | 67, 163 |
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| abstract_inverted_index.donors | 297 |
| abstract_inverted_index.either | 230 |
| abstract_inverted_index.embryo | 8, 15 |
| abstract_inverted_index.freeze | 84 |
| abstract_inverted_index.growth | 271 |
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| abstract_inverted_index.litter | 129 |
| abstract_inverted_index.morula | 97, 259 |
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| abstract_inverted_index.result | 159 |
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| abstract_inverted_index.viable | 385 |
| abstract_inverted_index.vitro, | 79, 115 |
| abstract_inverted_index.vitro. | 206 |
| abstract_inverted_index.16-cell | 151 |
| abstract_inverted_index.Another | 303 |
| abstract_inverted_index.amounts | 327 |
| abstract_inverted_index.animals | 339 |
| abstract_inverted_index.antigen | 251 |
| abstract_inverted_index.bearing | 238 |
| abstract_inverted_index.clearly | 177 |
| abstract_inverted_index.culture | 157 |
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| abstract_inverted_index.disease | 313 |
| abstract_inverted_index.efforts | 358 |
| abstract_inverted_index.embryos | 85, 94, 136, 147, 194, 254 |
| abstract_inverted_index.foreign | 263, 280, 329 |
| abstract_inverted_index.genetic | 28, 45 |
| abstract_inverted_index.hatched | 77 |
| abstract_inverted_index.mammary | 284 |
| abstract_inverted_index.morulae | 202 |
| abstract_inverted_index.nuclear | 186 |
| abstract_inverted_index.oocytes | 208 |
| abstract_inverted_index.piglets | 68, 164 |
| abstract_inverted_index.possess | 109 |
| abstract_inverted_index.produce | 279 |
| abstract_inverted_index.reduced | 132 |
| abstract_inverted_index.region, | 335 |
| abstract_inverted_index.similar | 126 |
| abstract_inverted_index.through | 384 |
| abstract_inverted_index.various | 20 |
| abstract_inverted_index.whereas | 80, 116 |
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| abstract_inverted_index.advanced | 394 |
| abstract_inverted_index.attempts | 82 |
| abstract_inverted_index.bisected | 103 |
| abstract_inverted_index.capacity | 190 |
| abstract_inverted_index.compared | 133 |
| abstract_inverted_index.culture. | 224 |
| abstract_inverted_index.definite | 374 |
| abstract_inverted_index.general, | 388 |
| abstract_inverted_index.increase | 311, 352 |
| abstract_inverted_index.isolated | 145, 174 |
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| abstract_inverted_index.make-up. | 46 |
| abstract_inverted_index.obtained | 71, 342 |
| abstract_inverted_index.progress | 61 |
| abstract_inverted_index.proteins | 281 |
| abstract_inverted_index.recovery | 9 |
| abstract_inverted_index.research | 357 |
| abstract_inverted_index.surgical | 5 |
| abstract_inverted_index.transfer | 169, 187, 308, 354 |
| abstract_inverted_index.twinning | 138 |
| abstract_inverted_index.valuable | 299 |
| abstract_inverted_index.Pregnancy | 123 |
| abstract_inverted_index.analysing | 245 |
| abstract_inverted_index.cytometry | 242 |
| abstract_inverted_index.embryonic | 364 |
| abstract_inverted_index.establish | 363 |
| abstract_inverted_index.generated | 380 |
| abstract_inverted_index.identical | 33 |
| abstract_inverted_index.impaired. | 122 |
| abstract_inverted_index.material, | 29 |
| abstract_inverted_index.pellucida | 89 |
| abstract_inverted_index.potential | 113 |
| abstract_inverted_index.practical | 65 |
| abstract_inverted_index.promising | 304 |
| abstract_inverted_index.proteins. | 302 |
| abstract_inverted_index.resulting | 107 |
| abstract_inverted_index.subjected | 18 |
| abstract_inverted_index.transfer, | 11 |
| abstract_inverted_index.alteration | 42 |
| abstract_inverted_index.blastocyst | 99 |
| abstract_inverted_index.chimaeras. | 386 |
| abstract_inverted_index.chromosome | 237 |
| abstract_inverted_index.eight-cell | 175, 257 |
| abstract_inverted_index.expression | 247 |
| abstract_inverted_index.generation | 31 |
| abstract_inverted_index.indicating | 290 |
| abstract_inverted_index.preferably | 331 |
| abstract_inverted_index.procedure. | 350 |
| abstract_inverted_index.procedures | 6, 50, 390 |
| abstract_inverted_index.resistance | 314 |
| abstract_inverted_index.separation | 232 |
| abstract_inverted_index.stimulated | 211 |
| abstract_inverted_index.suggesting | 336 |
| abstract_inverted_index.transgenic | 275, 316, 338 |
| abstract_inverted_index.blastocysts | 76, 171, 204, 219 |
| abstract_inverted_index.blastomeres | 144 |
| abstract_inverted_index.demiembryos | 108 |
| abstract_inverted_index.development | 118, 273 |
| abstract_inverted_index.efficiency, | 355 |
| abstract_inverted_index.efficiently | 344 |
| abstract_inverted_index.established | 4 |
| abstract_inverted_index.multiplets, | 34 |
| abstract_inverted_index.spermatozoa | 239, 324 |
| abstract_inverted_index.totipotency | 180, 377 |
| abstract_inverted_index.underlining | 178 |
| abstract_inverted_index.application. | 66 |
| abstract_inverted_index.blastocysts. | 162 |
| abstract_inverted_index.blastomeres, | 176 |
| abstract_inverted_index.bloodstream, | 289 |
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| abstract_inverted_index.experimental | 55 |
| abstract_inverted_index.preservation | 26 |
| abstract_inverted_index.successfully | 267 |
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| abstract_inverted_index.approximately | 141 |
| abstract_inverted_index.determination | 37, 226 |
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| abstract_inverted_index.manipulations | 21 |
| abstract_inverted_index.reconstituted | 192 |
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| abstract_inverted_index.pharmaceutical | 301 |
| abstract_inverted_index.post-acrosomal | 334 |
| abstract_inverted_index.microsurgically | 104 |
| abstract_inverted_index.biotechnological | 389 |
| abstract_inverted_index.cryopreservation | 73 |
| abstract_inverted_index.parthenogenetically | 212 |
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| institutions_distinct_count | 2 |
| sustainable_development_goals[0].id | https://metadata.un.org/sdg/3 |
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| sustainable_development_goals[0].display_name | Good health and well-being |
| citation_normalized_percentile.value | 0.12045642 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |