Mapping the growth effect of previously hidden ubiquitin alleles using an overexpression based mutational scan Article Swipe
David Mavor
,
Daniel N. Bolon
,
Parul Mishra
·
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.1101/831503
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.1101/831503
Deep mutational scanning has emerged as a powerful, high throughput approach to determine the growth effect of thousands of alleles at once in bulk competition. However, to date only the growth effect of mutant alleles in isolation has been determined. Building off previous work, we have created a library of all possible single point mutations in ubiquitin and determined the growth effect of mutants overexpressed in the presence of a wild type allele. Using this scan, we explained over half of the previously missing mutants in the single allele scan by showing that they exhibit deleterious effects when co-expressed with wild type ubiquitin. Additionally, unlike the single allele growth effect, these overexpression growth effects were distributed across the entire protein. This overexpression scan methodology can identify likely dominant mutant effects in any essential gene and is highly complementary with traditional deep mutational scanning approaches.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/831503
- https://www.biorxiv.org/content/biorxiv/early/2019/11/14/831503.full.pdf
- OA Status
- green
- References
- 26
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2983633243
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W2983633243Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1101/831503Digital Object Identifier
- Title
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Mapping the growth effect of previously hidden ubiquitin alleles using an overexpression based mutational scanWork title
- Type
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preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
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2019Year of publication
- Publication date
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2019-11-08Full publication date if available
- Authors
-
David Mavor, Daniel N. Bolon, Parul MishraList of authors in order
- Landing page
-
https://doi.org/10.1101/831503Publisher landing page
- PDF URL
-
https://www.biorxiv.org/content/biorxiv/early/2019/11/14/831503.full.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
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-
greenOpen access status per OpenAlex
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https://www.biorxiv.org/content/biorxiv/early/2019/11/14/831503.full.pdfDirect OA link when available
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Allele, Mutant, Biology, Genetics, Ubiquitin, Gene, Point mutation, Wild type, Mutation, Phenotype, Computational biologyTop concepts (fields/topics) attached by OpenAlex
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0Total citation count in OpenAlex
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26Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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| abstract_inverted_index.overexpression | 112, 122 |
| cited_by_percentile_year | |
| corresponding_author_ids | https://openalex.org/A5055119671 |
| countries_distinct_count | 2 |
| institutions_distinct_count | 3 |
| corresponding_institution_ids | https://openalex.org/I36893310 |
| citation_normalized_percentile.value | 0.10935808 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |