Mass cytometry reveals single-cell kinetics of cytotoxic lymphocyte evolution in CMV-infected renal transplant patients Article Swipe

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Mass cytometry Cytotoxic T cell Flow cytometry Immunology Renal transplant Lymphocyte Cytometry Kinetics Medicine Biology Transplantation Internal medicine Phenotype Genetics Physics In vitro Quantum mechanics Gene

Kenichi Ishiyama , Janice Arakawa‐Hoyt , Oscar A. Aguilar , Izabella Damm , Parhom Towfighi , Tara K. Sigdel , Stanley Tamaki , Joël Babdor , Matthew H. Spitzer , Elaine F. Reed , Minnie Sarwal , Lewis L. Lanier , Kenichi Ishiyama , Janice Arakawa‐Hoyt , Tara K. Sigdel , Elaine F. Reed , Minnie Sarwal , Lewis L. Lanier , Richard Ahn , Jenny Brook , Suphamai Bunnapradist , Nakul Datta , Mario C. Deng , Don J. Diamond , Tin Doung , Janette Gadzhyan , David Elashoff , David W. Gjertson , Victoria Groysberg , Alexander F. Hoffman , Erik L. Lum , Harry Pickering , Z. Qian , Michelle Ramírez , Maura Rossetti , Dimitri Rychov , Joanna Schaenman , Andrew Schroeder , Subha Sen , Danielle Sim , Marina Sirota , Flavio Vicenti , Otto O. Yang ·

YOU? · · 2022 · Open Access · · DOI: https://doi.org/10.1073/pnas.2116588119 · OA: W4212908527

Significance Memory-like NK cells (NKG2C + CD57 + FcεRIγ – ) are established during CMV infection. Here, mass cytometry tracked the in vivo kinetics of CMV-induced memory NK cells generation and identified a unique subset of NKG2C + CD57 + FcεRIγ low–dim as potentially prememory-like NK cells in CMV-infected kidney transplant patients. The study demonstrated that prememory-like NK cells with a high cytotoxic profile proliferate along with accumulation of new memory-like NK cells, whereas preexisting memory-like NK cells decreased in the peripheral blood after transplantation. Moreover, NKG2C + CD8 + T cells and cytotoxic γδ T cells also expand during CMV infection. This interplay of three different cytotoxic lymphocytes demonstrates a combinatorial immune response against CMV infection, which may contribute to preventing CMV-associated complication in organ transplantation.