Mastering the art of sectioning: a comprehensive guide to slide-microtome technology and histological applications Article Swipe
Yasmin M. Abd El‐Aziz
,
Mohammed S. Sobh
,
Helmi Ben Saad
,
Menna H. E. Morsy
,
Ehab El‐Haroun
,
Kasim Sakran Abass
,
Zulhisyam Abdul Kari
,
Roshmon Thomas Mathew
,
Moaheda E. H. Eissa
,
El‐Sayed Hemdan Eissa
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.3389/fvets.2025.1635706
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.3389/fvets.2025.1635706
The well-known technique of microtomy, which is an essential cutting tool, was first developed for light and transmission electron microscope uses, but it is currently also utilized to prepare specimens for atomic force microscopy (AFM), ion microscopy using a focused ion beam (FIB), and scanning electron microscopy (SEM). Ultramicrotomy can only be used on soft substances and metals that are sufficiently ductile to be cut with a diamond knife. Before being sliced by a microtome, many soft materials must first go through numerous preparatory processes. The choice of microtome type and blade material depends on the specimen being cut and the desired thickness of the sections. The scope of this review is to provide an overview of the main types of microtomes and a comparison between various common types of microtomes, recognizing and classifying frequent difficulties in tissue sample preparation with a particular emphasis on sectioning and staining problems. This review will put your focus on some problems, such as thick section borders, prolonged fixation or excess aldehyde concentration, low ethanol concentration or incomplete fixation, and sections not sticking to slides, especially during staining. In addition, explaining the artifacts during the processing of specimens using a microtome along with discussing the applications, calibration, maintenance of the microtome, as well as its troubleshooting, in addition to future trends, as well as challenges for this tool. Studying these issues will elevate key insights regarding the necessity of exact fixation, temperature control, accurate sectioning techniques, and gentle handling of tissues for the quality of histological samples used for microscopic studies.
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Metadata
- Type
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- Language
- en
- Landing Page
- https://doi.org/10.3389/fvets.2025.1635706
- https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2025.1635706/pdf
- OA Status
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- Cited By
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- References
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- Related Works
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- OpenAlex ID
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All OpenAlex metadata
Raw OpenAlex JSON
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https://openalex.org/W4413486558Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3389/fvets.2025.1635706Digital Object Identifier
- Title
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Mastering the art of sectioning: a comprehensive guide to slide-microtome technology and histological applicationsWork title
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reviewOpenAlex work type
- Language
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enPrimary language
- Publication year
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2025Year of publication
- Publication date
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2025-08-25Full publication date if available
- Authors
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Yasmin M. Abd El‐Aziz, Mohammed S. Sobh, Helmi Ben Saad, Menna H. E. Morsy, Ehab El‐Haroun, Kasim Sakran Abass, Zulhisyam Abdul Kari, Roshmon Thomas Mathew, Moaheda E. H. Eissa, El‐Sayed Hemdan EissaList of authors in order
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https://doi.org/10.3389/fvets.2025.1635706Publisher landing page
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https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2025.1635706/pdfDirect link to full text PDF
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goldOpen access status per OpenAlex
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https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2025.1635706/pdfDirect OA link when available
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Microtome, Anatomy, Computer science, Biology, Pathology, MedicineTop concepts (fields/topics) attached by OpenAlex
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1Total citation count in OpenAlex
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2025: 1Per-year citation counts (last 5 years)
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10Other works algorithmically related by OpenAlex
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