Matrix selection for the visualization of small molecules and lipids in brain tumors using untargeted MALDI-TOF mass spectrometry imaging Article Swipe
Tianyao Lu
,
Lutz Freytag
,
Vinod K. Narayana
,
Zachery Moore
,
Shannon J. Oliver
,
Adam Valkovic
,
Brunda Nijagal
,
Amanda L. Peterson
,
David P. De Souza
,
Malcolm J. McConville
,
James R. Whittle
,
Sarah A. Best
,
Saskia Freytag
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1101/2023.09.25.559427
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1101/2023.09.25.559427
Matrix-assisted laser desorption/ionization mass spectrometry imaging allows the study of metabolic activity in the tumor microenvironment of brain cancers. The detectable metabolites within these tumors are contingent upon the choice of matrix, deposition technique, and polarity setting. In this study, we compared the performance of three different matrices, two deposition techniques, and use of positive and negative polarity in two different brain cancer types and across two species. Optimal combinations were confirmed by comparative analysis of lipid and small molecule abundance using liquid chromatography–mass spectrometry and RNA-sequencing assessing differential metabolites between normal and tumor regions. Our findings indicate that the recrystallized α cyano-4-hydroxycinnamic acid matrix in positive polarity offered superior performance for both detected metabolites and consistency with other techniques. Beyond these implications for brain cancer, our work establishes a workflow to identify optimal matrices for spatial metabolomics studies.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2023.09.25.559427
- https://www.biorxiv.org/content/biorxiv/early/2023/09/25/2023.09.25.559427.full.pdf
- OA Status
- green
- Cited By
- 2
- References
- 26
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4387057528
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4387057528Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1101/2023.09.25.559427Digital Object Identifier
- Title
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Matrix selection for the visualization of small molecules and lipids in brain tumors using untargeted MALDI-TOF mass spectrometry imagingWork title
- Type
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preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
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2023Year of publication
- Publication date
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2023-09-25Full publication date if available
- Authors
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Tianyao Lu, Lutz Freytag, Vinod K. Narayana, Zachery Moore, Shannon J. Oliver, Adam Valkovic, Brunda Nijagal, Amanda L. Peterson, David P. De Souza, Malcolm J. McConville, James R. Whittle, Sarah A. Best, Saskia FreytagList of authors in order
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https://doi.org/10.1101/2023.09.25.559427Publisher landing page
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https://www.biorxiv.org/content/biorxiv/early/2023/09/25/2023.09.25.559427.full.pdfDirect link to full text PDF
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YesWhether a free full text is available
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greenOpen access status per OpenAlex
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https://www.biorxiv.org/content/biorxiv/early/2023/09/25/2023.09.25.559427.full.pdfDirect OA link when available
- Concepts
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Mass spectrometry imaging, Mass spectrometry, Metabolomics, Chemistry, Small molecule, Polarity (international relations), Matrix (chemical analysis), Computational biology, Chromatography, Biochemistry, Biology, CellTop concepts (fields/topics) attached by OpenAlex
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2Total citation count in OpenAlex
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2025: 1, 2024: 1Per-year citation counts (last 5 years)
- References (count)
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26Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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