Measurement of Larval Activity in the <em>Drosophila </em>Activity Monitor Article Swipe
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· 2015
· Open Access
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· DOI: https://doi.org/10.3791/52684
Drosophila larvae are used in many behavioral studies, yet a simple device for measuring basic parameters of larval activity has not been available. This protocol repurposes an instrument often used to measure adult activity, the TriKinetics Drosophila activity monitor (MB5 Multi-Beam Activity Monitor) to study larval activity. The instrument can monitor the movements of animals in 16 individual 8 cm glass assay tubes, using 17 infrared detection beams per tube. Logging software automatically saves data to a computer, recording parameters such as number of moves, times sensors were triggered, and animals' positions within the tubes. The data can then be analyzed to represent overall locomotion and/or position preference as well as other measurements. All data are easily accessible and compatible with basic graphing and data manipulation software. This protocol will discuss how to use the apparatus, how to operate the software and how to run a larval activity assay from start to finish.
Related Topics
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3791/52684
- OA Status
- green
- Cited By
- 9
- References
- 13
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W1999967648
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W1999967648Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.3791/52684Digital Object Identifier
- Title
-
Measurement of Larval Activity in the <em>Drosophila </em>Activity MonitorWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2015Year of publication
- Publication date
-
2015-04-30Full publication date if available
- Authors
-
Aidan McParland, Taylor Follansbee, Geoffrey K. GanterList of authors in order
- Landing page
-
https://doi.org/10.3791/52684Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
-
https://www.ncbi.nlm.nih.gov/pmc/articles/4542324Direct OA link when available
- Concepts
-
Activity monitor, Software, Protocol (science), Drosophila (subgenus), Data logger, Larva, Computer science, Neural activity, Real-time computing, Computer hardware, Biology, Neuroscience, Physical activity, Ecology, Medicine, Operating system, Pathology, Gene, Biochemistry, Physical medicine and rehabilitation, Alternative medicineTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
9Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 3, 2022: 1, 2020: 1, 2019: 2, 2015: 2Per-year citation counts (last 5 years)
- References (count)
-
13Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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