Metabolically active bacteria detected with click chemistry in low organic matter rainwater Article Swipe
Ryan Guillemette
,
Matthew C. Harwell
,
Cheryl Brown
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1371/journal.pone.0285816
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1371/journal.pone.0285816
Rain contains encapsulated bacteria that can be transported over vast distances during relatively short periods of time. However, the ecological significance of bacteria in “precontact” rainwater–rainwater prior to contact with non-atmospheric surfaces–remains relatively undefined given the methodological challenges of studying low-abundance microbes in a natural assemblage. Here, we implement single-cell “click” chemistry in a novel application to detect the protein synthesis of bacteria in precontact rainwater samples as a measure of metabolic activity. Using epifluorescence microscopy, we find approximately 10 3 –10 4 bacteria cells mL -1 with up to 7.2% of the observed cells actively synthesizing protein. Additionally, our measurement of less than 30 μM total organic carbon in the samples show that some rainwater bacteria can metabolize substrates in very low organic matter conditions, comparable to extremophiles in the deep ocean. Overall, our results raise new questions for the field of rainwater microbiology and may help inform efforts to develop quantitative microbial risk assessments for the appropriate use of harvested rainwater.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1371/journal.pone.0285816
- https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0285816&type=printable
- OA Status
- gold
- Cited By
- 2
- References
- 51
- Related Works
- 10
- OpenAlex ID
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All OpenAlex metadata
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- DOI
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https://doi.org/10.1371/journal.pone.0285816Digital Object Identifier
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Metabolically active bacteria detected with click chemistry in low organic matter rainwaterWork title
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2023Year of publication
- Publication date
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2023-05-18Full publication date if available
- Authors
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Ryan Guillemette, Matthew C. Harwell, Cheryl BrownList of authors in order
- Landing page
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https://doi.org/10.1371/journal.pone.0285816Publisher landing page
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https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0285816&type=printableDirect link to full text PDF
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YesWhether a free full text is available
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goldOpen access status per OpenAlex
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https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0285816&type=printableDirect OA link when available
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Rainwater harvesting, Bacteria, Environmental chemistry, Environmental science, Microorganism, Organic matter, Dissolved organic carbon, Chemistry, Biology, Ecology, GeneticsTop concepts (fields/topics) attached by OpenAlex
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2Total citation count in OpenAlex
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2025: 1, 2024: 1Per-year citation counts (last 5 years)
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51Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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