metaSpectraST: an unsupervised and database-independent analysis workflow for metaproteomic MS/MS data using spectrum clustering Article Swipe
Chunlin Hao
,
Joshua E. Elias
,
Patrick K. H. Lee
,
Henry Lam
·
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.21203/rs.3.rs-1695549/v1
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.21203/rs.3.rs-1695549/v1
Background: The high diversity and complexity of the microbial community make it a formidable challenge to identify and quantify the large number of proteins expressed in the community. Conventional metaproteomics approaches largely rely on accurate identification of the MS/MS spectra to their corresponding short peptides in the digested samples, followed by protein inference and subsequent taxonomic and functional analysis of the detected proteins. These approaches are dependent on the availability of protein sequence databases derived either from metagenomic sequencing of the same sample, or from public repositories. Due to the incompleteness or imperfections of these protein sequence databases, and the preponderance of homologous proteins expressed by different bacterial species in the community, this computational process of peptide identification and protein inference is challenging and error-prone, which hinders the comparison of metaproteomes across multiple samples. Results: We developed metaSpectraST, an unsupervised and database-independent workflow to analyze metaproteomic samples by clustering experimentally observed MS/MS spectra based on their spectral similarity. We applied metaSpectraST to fecal samples collected from littermates of two different mother mice right after weaning. Quantitative proteome profiles of the microbial communities of different mice could be obtained without peptide identification, and used to evaluate the overall similarity between samples and highlight any differentiating markers. Compared to the conventional database-dependent metaproteomics analysis, metaSpectraST is more successful in classifying the samples and detecting the subtle microbiome changes of mouse gut microbiomes post-weaning. metaSpectraST could also be used as a tool for selecting the suitable biological replicates from samples with wide inter-individual variation. Conclusions: metaSpectraST enables rapid profiling of metaproteomic samples without the need for constructing the protein sequence database or identification of the MS/MS spectra. It maximally preserves information contained in the experimental MS/MS spectra by clustering all of them first, and thus is able to better profile the complex microbial communities and highlight their functional changes, as compared with conventional approaches.
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- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.21203/rs.3.rs-1695549/v1
- https://www.researchsquare.com/article/rs-1695549/latest.pdf
- OA Status
- green
- Cited By
- 2
- References
- 62
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4281751814
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Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4281751814Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.21203/rs.3.rs-1695549/v1Digital Object Identifier
- Title
-
metaSpectraST: an unsupervised and database-independent analysis workflow for metaproteomic MS/MS data using spectrum clusteringWork title
- Type
-
preprintOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2022Year of publication
- Publication date
-
2022-05-31Full publication date if available
- Authors
-
Chunlin Hao, Joshua E. Elias, Patrick K. H. Lee, Henry LamList of authors in order
- Landing page
-
https://doi.org/10.21203/rs.3.rs-1695549/v1Publisher landing page
- PDF URL
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https://www.researchsquare.com/article/rs-1695549/latest.pdfDirect link to full text PDF
- Open access
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YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
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https://www.researchsquare.com/article/rs-1695549/latest.pdfDirect OA link when available
- Concepts
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Metaproteomics, Proteome, Metagenomics, Cluster analysis, Computational biology, Biology, Protein sequencing, Microbiome, Identification (biology), Inference, Similarity (geometry), Computer science, Bioinformatics, Artificial intelligence, Peptide sequence, Genetics, Image (mathematics), Botany, GeneTop concepts (fields/topics) attached by OpenAlex
- Cited by
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2Total citation count in OpenAlex
- Citations by year (recent)
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2025: 2Per-year citation counts (last 5 years)
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62Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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