Microbiology Article Swipe
YOU?
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· 2021
· Open Access
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· DOI: https://doi.org/10.14379/omandp.proc.119.2020
· OA: W4285474778
Hole GT3AA whole-round gabbro sample was collected from Core 131Z in Hole GT3A for DNA/RNA and lipid extractions.Samples were taken from the inside and outside of the core, as well as water from the rock saw used to cut the core.• 3 replicates from inside of core (~0.25-0.5 g crushed rock).• 3 replicates from outside of core (~0.25-0.5 g crushed rock).• 3 replicates of ~5 mL of water from the rock saw used to cut the core interval.• 3 replicates of both inner and outer core were collected in cryovials and frozen for transport for possible metabolomic or single-cell genomic work in the future. Hole BT1BA whole-round serpentine sample was collected from Core 39Z in Hole BT1B for DNA/RNA and lipid extractions.Samples were taken from the inside and outside of the core.• 3 replicates from inside of core (~0.25-0.5 g crushed rock).• 3 replicates from outside of core (~0.25-0.5 g crushed rock).• The remainder of the core was frozen at -80°C for future sampling needs. Sites BA1 and BA2Samples of drill cuttings were taken for DNA/RNA extraction and spectroscopic and bulk nitrogen analysis. DNA/RNA extractionCrushed drill cuttings samples were collected at a depth of 54 m.• 3 replicates (~0.25-0.5 g crushed rock).• 2 replicates of drilling foam/liquid (~20 mL).• An additional 3 replicates of crushed drill cuttings were collected in cryovials and frozen for transport for possible metabolomic or single-cell genomic work in the future. Spectroscopic analysis• Site BA1: ~15 g of drill cuttings were sampled from every 10 m at 10-140 m depth and every 20 m at 150-390 m depth.Additional samples of interest were also collected at 23, 58, 64, 65, 66, 144, 148, and 400 m depths.
